LOCUS       sequence-319492-       13368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE043 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13368)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE051 ZF43x1 mKate2 EF1a VP16-ZF43 EF1a
            EBFP...
FEATURES             Location/Qualifiers
     source          1..13368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3058..3067
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3064..3762
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3813..4037
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5086..6268
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5317..6259
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6369..6378
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6378..6443
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6450..6470
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7067..7291
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8340..9522
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8571..9513
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9623..9632
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9629..9631,9632..9634,9635..10345)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9674..9695)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10373..10429
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10442..10840
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10891..11115
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(11974..12783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12697..12716
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12775..12794
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(12942..13368,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13022..13041)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattagag tgaggacgat
     3001 ccctctagag ggtatataat gggggccact agtctactac cagagctcat cgctagcgcc
     3061 accatggtga gcgagctgat taaggagaac atgcacatga agctgtatat ggagggcacc
     3121 gtgaacaacc accacttcaa gtgtacctcc gagggcgaag gcaagcccta cgagggcacc
     3181 cagaccatga gaatcaaggc ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     3241 gctaccagct tcatgtacgg cagcaaaacc ttcatcaacc acacccaggg catccccgac
     3301 ttctttaagc agtccttccc cgagggcttc acatgggaga gagtcaccac atacgaagat
     3361 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     3421 gtcaagatca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
     3481 ggctgggagg cctccaccga aaccctgtac cccgctgacg gcggcctgga aggcagagcc
     3541 gacatggccc tgaagctcgt gggcgggggc cacctgatct gcaacttgaa aaccacatac
     3601 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactatgt ggacaggaga
     3661 ctggaaagaa tcaaggaggc cgacaaagaa acctacgtcg agcagcacga ggtggctgtg
     3721 gccagatact gcgacctccc tagcaaactg gggcacagat gagcggccgc tcgagtctag
     3781 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     3841 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     3901 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     3961 tggggtgggg caggacagca agggggagga ttgggaagtc aatagcaggc atgctgggga
     4021 tgcggtgggc tctatggctt ctgaggcagg tcgagggtac atgcgcctta ctccttgtga
     4081 gcgcatcgcg ataagctcta gagggacagc ccccccccaa agcccccagg gatgtaatta
     4141 cgtccctccc ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc
     4201 tccccccgca tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca
     4261 cgggatcgct ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg
     4321 atacggggaa aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc
     4381 ctgcgtacgg atggggctct agctctagag cttgatatcg aattcctgca gccccgctag
     4441 agggacagcc cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg
     4501 cagcagcgag ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg
     4561 gcagcgtgcg gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac
     4621 gcttctcgct gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg
     4681 ctgaaagaga gatttagaat gacagaatca tagaacggcc tgggcgcgcc tggtggcgaa
     4741 cgatcaacag acgggagagg gcaaaaccga tgttgacgga ctaatcctga caagatctgg
     4801 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     4861 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     4921 cagatatacg cgtgagtaat tcatacaaaa ggactcgccc ctgccttggg gaatcccagg
     4981 gaccgtcgtt aaactcccac taacgtagaa cccagagatc gctgcgttcc cgccccctca
     5041 cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg cgtgaggctc cggtgcccgt
     5101 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     5161 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     5221 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     5281 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     5341 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg
     5401 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     5461 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ttgggcgctg
     5521 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     5581 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     5641 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     5701 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     5761 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     5821 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     5881 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     5941 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     6001 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     6061 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     6121 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     6181 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     6241 tttttttctt ccatttcagg tgtcgtgaga gctcgtttag tgaaccgtca gatcgcctgg
     6301 agacgccatc cacgctgttt tgacctccat agaagagacc gggaccgatc caaattctcg
     6361 aggctagcgc caccatggat tataaggatc acgatggcga ctacaaggac cacgatattg
     6421 actacaaaga cgatgacgat aaaatggctc ctaagaaaaa gcgcaaagtc ggtatccatg
     6481 gcgttcccgt cgacggtgga ctgcaggccc caccaaccga tgtttcttta ggtgatgagt
     6541 tacatttgga cggtgaggac gtagctatgg cacatgcaga tgccttagac gattttgact
     6601 tggatatgtt aggagatgga gattcaccag gacctggttt cacaccacac gattcagctc
     6661 catacggtgc tttggacatg gccgatttcg aatttgaaca gatgtttact gatgccttgg
     6721 gtatagacga atacggtggt ggagctagac ccggggagcg ccccttccag tgtcgcattt
     6781 gcatgcggaa cttttcgcgc caggacaggc ttgacaggca tacccgtact cataccggtg
     6841 aaaaaccgtt tcagtgtcgg atctgtatgc gaaatttctc ccagaaggag cacttggcgg
     6901 ggcatctacg tacgcacacc ggcgagaagc cattccaatg ccgaatatgc atgcgcaact
     6961 tcagtcgccg cgacaacctg aaccggcacc taaaaaccca cctgagagga tcttaagcgg
     7021 ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt
     7081 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     7141 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     7201 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agtcaatagc
     7261 aggcatgctg gggatgcggt gggctctatg gcttctgagg caggtcgaag aactatttcc
     7321 tggctgttac gcgagcgcat cgcgataagc tctagaggga cagccccccc ccaaagcccc
     7381 cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg
     7441 ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca
     7501 cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc
     7561 agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag
     7621 aatcatagaa cggcctgcgt acggatgggg ctctagctct agagcttgat atcgaattcc
     7681 tgcagccccg ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     7741 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     7801 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     7861 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     7921 gaaaaagctt taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgggcg
     7981 cgcctggtgg cgaacgatca acagacggga gaggactaaa tagtagttca gacgccgtta
     8041 agcgcaagat ctggggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     8101 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     8161 cgcgatgtac gggccagata tacgcgtgag taattcatac aaaaggactc gcccctgcct
     8221 tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga gatcgctgcg
     8281 ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag catgcgtgag
     8341 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
     8401 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
     8461 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
     8521 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
     8581 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
     8641 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     8701 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     8761 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     8821 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     8881 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     8941 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     9001 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     9061 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     9121 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     9181 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     9241 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     9301 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     9361 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     9421 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     9481 acagtggttc aaagtttttt tcttccattt caggtgtcgt gagagctcgt ttagtgaacc
     9541 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga gaacgggacc
     9601 gatccaaatt ctcgaggcta gcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg
     9661 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgag
     9721 gggcgagggc gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac
     9781 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgagccacg gcgtgcagtg
     9841 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     9901 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcacctaca agacccgcgc
     9961 cgaggtgaag ttcgagggcg acaccctagt gaaccgcatc gagctgaagg gcgtcgactt
    10021 caaggaggac ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacat
    10081 ctatatcatg gccgtcaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
    10141 cgtggaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
    10201 cggccccgtg ctgctgcccg acagccacta cctgagcacc cagtccgtgc tgagcaaaga
    10261 ccccaacgag aagcgcgatc acatggtcct gctggagttc cgcaccgccg ccgggatcac
    10321 tctcggcatg gacgagctgt acaaggaatt cggtagcaag cttggtagtg gagcaactaa
    10381 cttttccctg ctcaaacaag ccggggatgt agaggagaac cccggccccg ccccccttaa
    10441 gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
    10501 caacagcatc cccatctctg aggactacag cgtcgccagc gcagctctct ctagcgacgg
    10561 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
    10621 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
    10681 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
    10741 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
    10801 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgctc gagtctagag
    10861 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
    10921 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
    10981 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
    11041 gggtggggca ggacagcaag ggggaggatt gggaagtcaa tagcaggcat gctggggatg
    11101 cggtgggctc tatggcttct gaggcaggtc gaagagactg cccgacacat cttagtgagc
    11161 gcatcgcgat aagctctaga gggacagccc ccccccaaag cccccaggga tgtaattacg
    11221 tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg tccggcgctc
    11281 cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg
    11341 ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac ctggggggat
    11401 acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat agaacggcct
    11461 gcgtacggat ggggctctag ctctagagct tgatatcgaa ttcctgcagc cccgctagag
    11521 ggacagcccc cccccaaagc ccccagggat gtaattacgt ccctcccccg ctagggggca
    11581 gcagcgagcc gcccggggct ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc
    11641 agcgtgcggg gacagcccgg gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc
    11701 ttctcgctgc tctttgagcc tgcagacacc tggggggata cggggaaaaa gctttaggct
    11761 gaaagagaga tttagaatga cagaatcata gaacggcctg ggcgcgcctg gtggcgaacg
    11821 atcaacagac gggagaggtt acgaggatgc acatgtgacc gagggatgga acgacgcgta
    11881 actcacgtta agggattttg gtcatgagct tgcgccgtcc cgtcaagtca gcgtaatgct
    11941 ctgccagtgt tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg
    12001 aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg
    12061 taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc
    12121 tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag
    12181 gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagttt
    12241 atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact
    12301 cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc
    12361 gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag
    12421 cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt
    12481 tccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat
    12541 ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc
    12601 attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata
    12661 caagcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata
    12721 taaatcagca tccatgttgg aatttaatcg cggcctcgac gtttcccgtt gaatatggct
    12781 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
    12841 atttttatct tgtgcaatgt aacatcagag attttgagac acgggccatg ctagctccag
    12901 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    12961 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    13021 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    13081 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    13141 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    13201 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    13261 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    13321 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactac
//
