LOCUS       sequence-319494-       10099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE042 (TUPV1), pJM451 (TUPV2), 
            pPD612.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE058 ZF43x0 mKate2 EF1a EBFP2-P2A-BlastR
FEATURES             Location/Qualifiers
     source          1..10099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3043..3052
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3049..3747
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3798..4022
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5071..6253
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5302..6244
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6201..6221
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6255..6279
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      6354..6363
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6360..6362,6363..6365,6366..7076)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(6405..6426)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             7104..7160
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7173..7571
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    7622..7846
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(8705..9514)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9428..9447
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9506..9525
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(9673..10099,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9753..9772)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatct gaattcgaga ccacaggtct cggatccctc tagagggtat
     3001 ataatggggg ccactagtct actaccagag ctcatcgcta gcgccaccat ggtgagcgag
     3061 ctgattaagg agaacatgca catgaagctg tatatggagg gcaccgtgaa caaccaccac
     3121 ttcaagtgta cctccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     3181 aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     3241 tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     3301 ttccccgagg gcttcacatg ggagagagtc accacatacg aagatggggg cgtgctgacc
     3361 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     3421 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc
     3481 accgaaaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag
     3541 ctcgtgggcg ggggccacct gatctgcaac ttgaaaacca catacagatc caagaaaccc
     3601 gctaagaacc tcaagatgcc cggcgtctac tatgtggaca ggagactgga aagaatcaag
     3661 gaggccgaca aagaaaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     3721 ctccctagca aactggggca cagatgagcg gccgctcgag tctagagggc ccgtttaaac
     3781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     3841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     3901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     3961 cagcaagggg gaggattggg aagtcaatag caggcatgct ggggatgcgg tgggctctat
     4021 ggcttctgag gcaggtcgag ggtacatgcg ccttactcct tgtgagcgca tcgcgataag
     4081 ctctagaggg acagcccccc cccaaagccc ccagggatgt aattacgtcc ctcccccgct
     4141 agggggcagc agcgagccgc ccggggctcc gctccggtcc ggcgctcccc ccgcatcccc
     4201 gagccggcag cgtgcgggga cagcccgggc acggggaagg tggcacggga tcgctttcct
     4261 ctgaacgctt ctcgctgctc tttgagcctg cagacacctg gggggatacg gggaaaaagc
     4321 tttaggctga aagagagatt tagaatgaca gaatcataga acggcctgcg tacggatggg
     4381 gctctagctc tagagcttga tatcgaattc ctgcagcccc gctagaggga cagccccccc
     4441 ccaaagcccc cagggatgta attacgtccc tcccccgcta gggggcagca gcgagccgcc
     4501 cggggctccg ctccggtccg gcgctccccc cgcatccccg agccggcagc gtgcggggac
     4561 agcccgggca cggggaaggt ggcacgggat cgctttcctc tgaacgcttc tcgctgctct
     4621 ttgagcctgc agacacctgg ggggatacgg ggaaaaagct ttaggctgaa agagagattt
     4681 agaatgacag aatcatagaa cggcctgggc gcgcctggtg gcgaacgatc aacagacggg
     4741 agagggcaaa accgatgttg acggactaat cctgacaaga tctggggagg tcgctgagta
     4801 gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg accgacaatt gcatgaagaa
     4861 tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgtga
     4921 gtaattcata caaaaggact cgcccctgcc ttggggaatc ccagggaccg tcgttaaact
     4981 cccactaacg tagaacccag agatcgctgc gttcccgccc cctcacccgc ccgctctcgt
     5041 catcactgag gtggagaaga gcatgcgtga ggctccggtg cccgtcagtg ggcagagcgc
     5101 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     5161 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     5221 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     5281 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     5341 acgggttatg gcccttgcgt gccttgaatt acttccacgc ccctggctgc agtacgtgat
     5401 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     5461 agccccttcg cctcgtgctt gagttgaggc ctggcttggg cgctggggcc gccgcgtgcg
     5521 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     5581 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     5641 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     5701 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     5761 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     5821 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     5881 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     5941 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     6001 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     6061 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     6121 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     6181 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     6241 tcaggtgtcg tgagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     6301 tgttttgacc tccatagaag agaacgggac cgatccaaat tctcgaggct agcgccacca
     6361 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6421 gcgacgtaaa cggccacaag ttcagcgtga ggggcgaggg cgagggcgat gccaccaacg
     6481 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     6541 tcgtgaccac cctgagccac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     6601 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6661 tcaaggacga cggcacctac aagacccgcg ccgaggtgaa gttcgagggc gacaccctag
     6721 tgaaccgcat cgagctgaag ggcgtcgact tcaaggagga cggcaacatc ctggggcaca
     6781 agctggagta caacttcaac agccacaaca tctatatcat ggccgtcaag cagaagaacg
     6841 gcatcaaggt gaacttcaag atccgccaca acgtggagga cggcagcgtg cagctcgccg
     6901 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacagccact
     6961 acctgagcac ccagtccgtg ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7021 tgctggagtt ccgcaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggaat
     7081 tcggtagcaa gcttggtagt ggagcaacta acttttccct gctcaaacaa gccggggatg
     7141 tagaggagaa ccccggcccc gcccccctta agatggccaa gcctttgtct caagaagaat
     7201 ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct gaggactaca
     7261 gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc
     7321 attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag
     7381 ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct
     7441 gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg
     7501 acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt
     7561 gggagggcta agcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     7621 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7681 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7741 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7801 tgggaagtca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcaggt
     7861 cgaagaacta tttcctggct gttacgcgag cgcatcgcga taagctctag agggacagcc
     7921 cccccccaaa gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag
     7981 ccgcccgggg ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg
     8041 gggacagccc gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct
     8101 gctctttgag cctgcagaca cctgggggga tacggggaaa aagctttagg ctgaaagaga
     8161 gatttagaat gacagaatca tagaacggcc tgcgtacgga tggggctcta gctctagagc
     8221 ttgatatcga attcctgcag ccccgctaga gggacagccc ccccccaaag cccccaggga
     8281 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     8341 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     8401 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     8461 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
     8521 agaacggcct gggcgcgcct ggtggcgaac gatcaacaga cgggagagga ctagaggatg
     8581 cacatgtgac cgagggatgg aacgacgcgt aactcacgtt aagggatttt ggtcatgagc
     8641 ttgcgccgtc ccgtcaagtc agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt
     8701 ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat
     8761 caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt
     8821 tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac
     8881 aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga
     8941 cgactgaatc cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag
     9001 gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg
     9061 attgcgcctg agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa
     9121 tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag
     9181 gatattcttc taatacctgg aatgctgttt ttccggggat cgcagtggtg agtaaccatg
     9241 catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc
     9301 agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca
     9361 gaaacaactc tggcgcatcg ggcttcccat acaagcgata gattgtcgca cctgattgcc
     9421 cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc
     9481 gcggcctcga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     9541 aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg taacatcaga
     9601 gattttgaga cacgggccat gctagctcca gcaaaaggcc aggaaccgta aaaaggccgc
     9661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     9721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     9781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     9841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     9901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     9961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10081 gaagtggtgg cctaactac
//
