LOCUS       sequence-319496-       13398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration Vector, assembled with pHIE044 (TUPV1), pJM466 (TUPV2), 
            pJM452 (TUPV3), pPD613.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13398)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 13398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pHIE052 ZF43x3-C mKate2 EF1a VP16-ZF43 EF1a
            EB...
FEATURES             Location/Qualifiers
     source          1..13398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1031..1068
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             1079..1678
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1079..1098)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1575..1595
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(1791..1810)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    1856..1911
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1910..1929)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     regulatory      3088..3097
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3094..3792
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     polyA_signal    3843..4067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        5116..6298
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5347..6289
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      6399..6408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6408..6473
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             6480..6500
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7097..7321
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        8370..9552
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          8601..9543
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     regulatory      9653..9662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9659..9661,9662..9664,9665..10375)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(9704..9725)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             10403..10459
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             10472..10870
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    10921..11145
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(12004..12813)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12727..12746
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     12805..12824
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(join(12972..13398,1..162))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13052..13071)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
       61 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      241 ttatcaaaaa ggatcttcac ctagatcctt tctcgagctt tcggagtgag atggagtggc
      301 cactgtggaa ctgggcctag catcgcgata agctctagag ggacagcccc cccccaaagc
      361 ccccagggat gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct
      421 ccgctccggt ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg
      481 gcacggggaa ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc
      541 tgcagacacc tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga
      601 cagaatcata gaacggcctg cgtacggatg gggctctagc tctagagctt gatatcgaat
      661 tcctgcagcc ccgctagagg gacagccccc ccccaaagcc cccagggatg taattacgtc
      721 cctcccccgc tagggggcag cagcgagccg cccggggctc cgctccggtc cggcgctccc
      781 cccgcatccc cgagccggca gcgtgcgggg acagcccggg cacggggaag gtggcacggg
      841 atcgctttcc tctgaacgct tctcgctgct ctttgagcct gcagacacct ggggggatac
      901 ggggaaaaag ctttaggctg aaagagagat ttagaatgac agaatcatag aacggcctgg
      961 gcgcgcctgg tggcgaacga tcaacagacg ggagatctac tattgggaca gcagagatcc
     1021 agttggtaat ggcttgtcga cgacggcggt ctccgtcgtc aggatcatag gtgccaccat
     1081 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtccccc gggccgtacg
     1141 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg acccggaccg
     1201 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     1261 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     1321 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     1381 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     1441 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     1501 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     1561 gacatccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     1621 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagc
     1681 ttccactgga ttgtacaatt accaactgaa ttcactcctc aggtgcaggc tgcctatcag
     1741 aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga tctttttccc
     1801 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     1861 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     1921 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     1981 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     2041 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     2101 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     2161 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     2221 cttctcttat ggagatccgg gcctagcatc gcgataagct ctagagggac agcccccccc
     2281 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     2341 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     2401 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     2461 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     2521 gaatgacaga atcatagaac ggcctgcgta cggatggggc tctagctcta gagcttgata
     2581 tcgaattcct gcagccccgc tagagggaca gccccccccc aaagccccca gggatgtaat
     2641 tacgtccctc ccccgctagg gggcagcagc gagccgcccg gggctccgct ccggtccggc
     2701 gctccccccg catccccgag ccggcagcgt gcggggacag cccgggcacg gggaaggtgg
     2761 cacgggatcg ctttcctctg aacgcttctc gctgctcttt gagcctgcag acacctgggg
     2821 ggatacgggg aaaaagcttt aggctgaaag agagatttag aatgacagaa tcatagaacg
     2881 gcctgggcgc gcctggtggc gaacgatcaa cagacgggag attgccaagg tcttgacaaa
     2941 cgagtgctag tacaagatcg cgtcgacgga tcgggagatc tgaattagag tgaggacgat
     3001 cggagtgagg ataattagag tgaggacgat ccctctagag ggtatataat gggggccact
     3061 agtctactac cagagctcat cgctagcgcc accatggtga gcgagctgat taaggagaac
     3121 atgcacatga agctgtatat ggagggcacc gtgaacaacc accacttcaa gtgtacctcc
     3181 gagggcgaag gcaagcccta cgagggcacc cagaccatga gaatcaaggc ggtcgagggc
     3241 ggccctctcc ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcaaaacc
     3301 ttcatcaacc acacccaggg catccccgac ttctttaagc agtccttccc cgagggcttc
     3361 acatgggaga gagtcaccac atacgaagat gggggcgtgc tgaccgctac ccaggacacc
     3421 agcctccagg acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc
     3481 aacggccctg tgatgcagaa gaaaacactc ggctgggagg cctccaccga aaccctgtac
     3541 cccgctgacg gcggcctgga aggcagagcc gacatggccc tgaagctcgt gggcgggggc
     3601 cacctgatct gcaacttgaa aaccacatac agatccaaga aacccgctaa gaacctcaag
     3661 atgcccggcg tctactatgt ggacaggaga ctggaaagaa tcaaggaggc cgacaaagaa
     3721 acctacgtcg agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg
     3781 gggcacagat gagcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     3841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     3901 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     3961 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     4021 ttgggaagtc aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcagg
     4081 tcgagggtac atgcgcctta ctccttgtga gcgcatcgcg ataagctcta gagggacagc
     4141 ccccccccaa agcccccagg gatgtaatta cgtccctccc ccgctagggg gcagcagcga
     4201 gccgcccggg gctccgctcc ggtccggcgc tccccccgca tccccgagcc ggcagcgtgc
     4261 ggggacagcc cgggcacggg gaaggtggca cgggatcgct ttcctctgaa cgcttctcgc
     4321 tgctctttga gcctgcagac acctgggggg atacggggaa aaagctttag gctgaaagag
     4381 agatttagaa tgacagaatc atagaacggc ctgcgtacgg atggggctct agctctagag
     4441 cttgatatcg aattcctgca gccccgctag agggacagcc cccccccaaa gcccccaggg
     4501 atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg ctccgctccg
     4561 gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc gggcacgggg
     4621 aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag cctgcagaca
     4681 cctgggggga tacggggaaa aagctttagg ctgaaagaga gatttagaat gacagaatca
     4741 tagaacggcc tgggcgcgcc tggtggcgaa cgatcaacag acgggagagg gcaaaaccga
     4801 tgttgacgga ctaatcctga caagatctgg ggaggtcgct gagtagtgcg cgagcaaaat
     4861 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     4921 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtgagtaat tcatacaaaa
     4981 ggactcgccc ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa
     5041 cccagagatc gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga
     5101 gaagagcatg cgtgaggctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc
     5161 cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag gtggcgcggg
     5221 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa
     5281 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga
     5341 acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct
     5401 tgcgtgcctt gaattacttc cacgcccctg gctgcagtac gtgattcttg atcccgagct
     5461 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     5521 tgcttgagtt gaggcctggc ttgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     5581 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     5641 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     5701 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     5761 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     5821 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     5881 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     5941 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     6001 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     6061 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     6121 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     6181 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     6241 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgaga
     6301 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     6361 agaagagacc gggaccgatc caaattctcg aggctagcgc caccatggat tataaggatc
     6421 acgatggcga ctacaaggac cacgatattg actacaaaga cgatgacgat aaaatggctc
     6481 ctaagaaaaa gcgcaaagtc ggtatccatg gcgttcccgt cgacggtgga ctgcaggccc
     6541 caccaaccga tgtttcttta ggtgatgagt tacatttgga cggtgaggac gtagctatgg
     6601 cacatgcaga tgccttagac gattttgact tggatatgtt aggagatgga gattcaccag
     6661 gacctggttt cacaccacac gattcagctc catacggtgc tttggacatg gccgatttcg
     6721 aatttgaaca gatgtttact gatgccttgg gtatagacga atacggtggt ggagctagac
     6781 ccggggagcg ccccttccag tgtcgcattt gcatgcggaa cttttcgcgc caggacaggc
     6841 ttgacaggca tacccgtact cataccggtg aaaaaccgtt tcagtgtcgg atctgtatgc
     6901 gaaatttctc ccagaaggag cacttggcgg ggcatctacg tacgcacacc ggcgagaagc
     6961 cattccaatg ccgaatatgc atgcgcaact tcagtcgccg cgacaacctg aaccggcacc
     7021 taaaaaccca cctgagagga tcttaagcgg ccgctcgagt ctagagggcc cgtttaaacc
     7081 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     7141 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     7201 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     7261 agcaaggggg aggattggga agtcaatagc aggcatgctg gggatgcggt gggctctatg
     7321 gcttctgagg caggtcgaag aactatttcc tggctgttac gcgagcgcat cgcgataagc
     7381 tctagaggga cagccccccc ccaaagcccc cagggatgta attacgtccc tcccccgcta
     7441 gggggcagca gcgagccgcc cggggctccg ctccggtccg gcgctccccc cgcatccccg
     7501 agccggcagc gtgcggggac agcccgggca cggggaaggt ggcacgggat cgctttcctc
     7561 tgaacgcttc tcgctgctct ttgagcctgc agacacctgg ggggatacgg ggaaaaagct
     7621 ttaggctgaa agagagattt agaatgacag aatcatagaa cggcctgcgt acggatgggg
     7681 ctctagctct agagcttgat atcgaattcc tgcagccccg ctagagggac agcccccccc
     7741 caaagccccc agggatgtaa ttacgtccct cccccgctag ggggcagcag cgagccgccc
     7801 ggggctccgc tccggtccgg cgctcccccc gcatccccga gccggcagcg tgcggggaca
     7861 gcccgggcac ggggaaggtg gcacgggatc gctttcctct gaacgcttct cgctgctctt
     7921 tgagcctgca gacacctggg gggatacggg gaaaaagctt taggctgaaa gagagattta
     7981 gaatgacaga atcatagaac ggcctgggcg cgcctggtgg cgaacgatca acagacggga
     8041 gaggactaaa tagtagttca gacgccgtta agcgcaagat ctggggaggt cgctgagtag
     8101 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
     8161 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgtgag
     8221 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     8281 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     8341 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     8401 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     8461 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     8521 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     8581 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     8641 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     8701 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     8761 gccccttcgc ctcgtgcttg agttgaggcc tggcttgggc gctggggccg ccgcgtgcga
     8821 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     8881 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     8941 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     9001 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     9061 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     9121 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     9181 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     9241 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     9301 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     9361 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     9421 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     9481 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     9541 caggtgtcgt gagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     9601 gttttgacct ccatagaaga gaacgggacc gatccaaatt ctcgaggcta gcgccaccat
     9661 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     9721 cgacgtaaac ggccacaagt tcagcgtgag gggcgagggc gagggcgatg ccaccaacgg
     9781 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     9841 cgtgaccacc ctgagccacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
     9901 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     9961 caaggacgac ggcacctaca agacccgcgc cgaggtgaag ttcgagggcg acaccctagt
    10021 gaaccgcatc gagctgaagg gcgtcgactt caaggaggac ggcaacatcc tggggcacaa
    10081 gctggagtac aacttcaaca gccacaacat ctatatcatg gccgtcaagc agaagaacgg
    10141 catcaaggtg aacttcaaga tccgccacaa cgtggaggac ggcagcgtgc agctcgccga
    10201 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acagccacta
    10261 cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
    10321 gctggagttc cgcaccgccg ccgggatcac tctcggcatg gacgagctgt acaaggaatt
    10381 cggtagcaag cttggtagtg gagcaactaa cttttccctg ctcaaacaag ccggggatgt
    10441 agaggagaac cccggccccg ccccccttaa gatggccaag cctttgtctc aagaagaatc
    10501 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aggactacag
    10561 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
    10621 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
    10681 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
    10741 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
    10801 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
    10861 ggagggctaa gcggccgctc gagtctagag ggcccgttta aacccgctga tcagcctcga
    10921 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10981 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    11041 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    11101 gggaagtcaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcaggtc
    11161 gaagagactg cccgacacat cttagtgagc gcatcgcgat aagctctaga gggacagccc
    11221 ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc agcagcgagc
    11281 cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg
    11341 ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg cttctcgctg
    11401 ctctttgagc ctgcagacac ctggggggat acggggaaaa agctttaggc tgaaagagag
    11461 atttagaatg acagaatcat agaacggcct gcgtacggat ggggctctag ctctagagct
    11521 tgatatcgaa ttcctgcagc cccgctagag ggacagcccc cccccaaagc ccccagggat
    11581 gtaattacgt ccctcccccg ctagggggca gcagcgagcc gcccggggct ccgctccggt
    11641 ccggcgctcc ccccgcatcc ccgagccggc agcgtgcggg gacagcccgg gcacggggaa
    11701 ggtggcacgg gatcgctttc ctctgaacgc ttctcgctgc tctttgagcc tgcagacacc
    11761 tggggggata cggggaaaaa gctttaggct gaaagagaga tttagaatga cagaatcata
    11821 gaacggcctg ggcgcgcctg gtggcgaacg atcaacagac gggagaggtt acgaggatgc
    11881 acatgtgacc gagggatgga acgacgcgta actcacgtta agggattttg gtcatgagct
    11941 tgcgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt tacaaccaat taaccaattc
    12001 tgattagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc
    12061 aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt
    12121 ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca
    12181 acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac
    12241 gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg
    12301 ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga
    12361 ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat
    12421 cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg
    12481 atattcttct aatacctgga atgctgtttt tccggggatc gcagtggtga gtaaccatgc
    12541 atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca
    12601 gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag
    12661 aaacaactct ggcgcatcgg gcttcccata caagcgatag attgtcgcac ctgattgccc
    12721 gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg
    12781 cggcctcgac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta
    12841 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag
    12901 attttgagac acgggccatg ctagctccag caaaaggcca ggaaccgtaa aaaggccgcg
    12961 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    13021 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    13081 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    13141 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    13201 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    13261 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    13321 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    13381 aagtggtggc ctaactac
//
