LOCUS       sequence-31951-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Johnson TK, Schweppe RE, Septer J, Lewis RE
  TITLE     Phosphorylation of B-Myb regulates its transactivation potential and
            DNA binding.
  JOURNAL   J Biol Chem. 1999 Dec 17. 274(51):36741-9.
  PUBMED    10593981
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Dec 17. 274(51):36741-9."
COMMENT     Sequence Label: pCDNA3 10 mut B-Myb
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     56..75
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        56..74
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      137..146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             146..169
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tttagcagag ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata
       61 cgactcacta tagggagacc caagcttggt accgagctcg gatccactag taacggccgc
      121 cagtgtgctg gaattcgcca ccatggacta caaggacgac gatgacaagt ctcggcggac
      181 gcgctgcgag gatctggatg agttacacta ccaggacgtg gactcagacc tcctggagca
      241 gagagacaac agatgtaagg ttaaatggac ccacgaggag gacgagcagt tgagggccct
      301 ggtgaggcag tttggacagc aagactggaa gttcttggcc agtcactttc ctaaccgcac
      361 agaccagcaa tgccagtacc ggtggctgag ggttttgaat cccgaccttg ttaagggacc
      421 atggaccaaa gaggaagatc aaaaggtcat tgagttggtc aagaagtatg gcaccaaaca
      481 gtggacgctg atagccaagc acctgaaggg ccggctgggg aagcagtgcc gtgagcgctg
      541 gcacaaccac ctcaaccccg aggtgaagaa gtcatgctgg accgaggagg aagacaggat
      601 aatctgtgag gcccataaag tcctgggtaa ccgctgggct gagatcgcca agatgctacc
      661 agggaggacg gacaatgctg tgaagaatca ctggaactcc actatcaaaa ggaaagtcga
      721 cacgggaggt ttcccagccg agtccaggga ctgcaagcct gtctacttgc tcctggagct
      781 ggaggacaag gaacagcacc agggtgtcca gccggtggac ggccagggaa gtctcgtgag
      841 cagctggccg ctggtgccct ctattgtgaa ggaggagagc agcgaggagg agattgccat
      901 agctgctact tctgtagatc ga
//
