LOCUS       sequence-31954-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry vector containing BFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Yang X, Boehm JS, Yang X, Salehi-Ashtiani K, Hao T, Shen Y, Lubonja 
            R, Thomas SR, Alkan O, Bhimdi T, Green TM, Johannessen CM, Silver 
            SJ, Nguyen C, Murray RR, Hieronymus H, Balcha D, Fan C, Lin C, 
            Ghamsari L, Vidal M, Hahn WC, Hill DE, Root DE
  TITLE     A public genome-scale lentiviral expression library of human ORFs.
  JOURNAL   Nat Methods. 2011 Jun 26;8(8):659-61. doi: 10.1038/nmeth.1638
  PUBMED    21706014
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2011-06-26- 26"; volume: "8"; 
            issue: "8"; pages: "659-61"
COMMENT     Sequence Label: pDONR223_BFP
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    12..111
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(155..176)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     504..523
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     763..784
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cagctggatg gcaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
       61 ttgataagca atgctttctt ataatgccaa ctttgtacaa gaaagttggc aacttgtaca
      121 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
      181 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggctgtcgg
      241 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
      301 cgctgccgtc ctcgatgttg tggcggatct tgaagttcgc cttgatgccg ttcttctgct
      361 tgtcggccat gatatagacg ttgtggctgt tgaagttgta ctccagcttg tgccccagga
      421 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
      481 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
      541 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
      601 cggggtagcg gctgaagcac tggacgccgt ggctcagggt ggtcacgagg gtgggccagg
      661 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
      721 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
      781 ccaggatggg ca
//
