LOCUS       sequence-319553-        9103 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses TTYH1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9103)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TTYH1_OE_1
FEATURES             Location/Qualifiers
     source          1..9103
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1937..1961)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1963..2004
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2046..2634
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2099..2119)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2517..2528)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2706..2939
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3017..3138
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3054..3073)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3108..3127
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3178..3313
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3333..3352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3334..3352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3362..3379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3362..3378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3371..3393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3520..3975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3607..3626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3817..3838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4001..4105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4106..4174,4175..4966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4324..4343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5137..5725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5626..5645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5879..5896
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6013..6034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6049..6054,6055..6072,6073..6079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6087..6103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6092..6114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6111..6127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6111..6127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6146..6166
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6148..6166
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6194..6420
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6421..6601
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6648..6773
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7266..7499
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7684..7728
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7903..8403
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8322..8340
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8415..8810
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8870..8987
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggctatggg ggcgcccccg ggctaccggc cctcagcttg ggtgcatctc
      601 ctccaccagc tgccccgcgc cgacttccag ctccgcccgg tgcccagcgt tttcgcgccc
      661 caagagcagg aataccagca ggccttgttg ctggtggcgg ccttggcggg cctgggcttg
      721 ggcctgagcc tcattttcat cgctgtctac ctcatccgct tctgctgctg ccggcccccc
      781 gagccccccg ggtccaagat cccctcgccc gggggaggct gcgtcacctg gagctgcatt
      841 gtcgcccttc tcgccggctg cactggcatt ggcatcggtt tctatggcaa cagtgagacc
      901 agtgatgggg tgtcccagct cagctctgcg ctgctgcacg ccaaccacac actcagcacc
      961 attgaccacc tggtgttgga gacggtggag aggctgggcg aggcggtgag gacagagctg
     1021 accaccctgg aggaggtgct cgagccgcgc acggagctgg tggctgccgc ccgaggggct
     1081 cgacggcagg cggaggctgc ggcccagcag ctgcaggggc tggccttctg gcagggagtg
     1141 cccctgagcc ccctgcaggt ggctgaaaat gtgtcctttg tggaggagta caggtggctg
     1201 gcctacgtcc tcctgctgct cctggagctg ctggtctgcc tcttcaccct cctgggcctg
     1261 gcgaagcaga gcaagtggct ggtgatcgtg atgacagtca tgagtctcct ggttctcgtc
     1321 ctgagctggg gctccatggg cctggaggca gccacggccg tgggcctcag tgacttctgc
     1381 tccaatccag acccttatgt tctgaacctg acccaggagg agacagggct cagctcagac
     1441 atcctgagct attatctcct ctgcaaccgg gccgtctcca accccttcca acagaggctg
     1501 actctgtccc agcgagctct ggccaacatc cactcccagc tgctgggcct ggagcgagaa
     1561 gctgtgcctc agttcccttc agcgcagaag cctctgctgt ccttggagga gactctgaat
     1621 gtgacagaag gaaatttcca ccagttggtg gcactgctac actgccgcag cctgcacaag
     1681 gactatggtg cagccctgcg gggcctgtgc gaagacgccc tggaaggcct gctcttcctg
     1741 ctactcttct ccctgctgtc tgcaggagcg ctggccactg ccctctgcag cctgccccga
     1801 gcctgggccc tcttcccacc caggaatcca agcgctttgt gcagtggcag tcgtctatct
     1861 gagcccctcc tcccggctgg actggagcct ggctcccctc ttcgttcctt ccctggctgc
     1921 cggaggagac cccactaccc agctttcttg tacaaagtgg ttggtaagcc tatccctaac
     1981 cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag
     2041 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2101 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2161 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2221 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     2281 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     2341 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     2401 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc
     2461 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     2521 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     2581 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac
     2641 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     2701 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     2761 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     2821 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     2881 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     2941 tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     3001 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3061 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3121 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     3181 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     3241 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     3301 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     3361 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     3421 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     3481 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     3541 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3601 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3661 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     3721 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     3781 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     3841 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3901 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3961 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4021 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4081 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     4141 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     4201 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     4261 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     4321 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     4381 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     4441 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     4501 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     4561 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     4621 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     4681 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     4741 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     4801 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     4861 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4921 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4981 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5041 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     5101 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     5161 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     5221 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     5281 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     5341 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     5401 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     5461 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     5521 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     5581 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     5641 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     5701 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     5761 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     5821 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     5881 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     5941 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     6001 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     6061 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6121 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     6181 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     6241 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     6301 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     6361 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     6421 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6481 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6541 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6601 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     6661 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     6721 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     6781 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     6841 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     6901 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     6961 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     7021 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     7081 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     7141 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     7201 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     7261 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     7321 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     7381 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     7441 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     7501 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     7561 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     7621 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     7681 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     7741 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     7801 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     7861 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc
     7921 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
     7981 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
     8041 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
     8101 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
     8161 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
     8221 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
     8281 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
     8341 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
     8401 cagggggtac caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag
     8461 caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc
     8521 tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt
     8581 gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta
     8641 tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg
     8701 tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga
     8761 cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc
     8821 agtagtaaga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
     8881 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     8941 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
     9001 gggacagcag agatccactt tggcgccggc tcgagggggc ccggggttat taatagtaat
     9061 caattacggg gtcattagtt catagcccat atatggagtt ccg
//
