LOCUS       sequence-319558-        8394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses SLC26A1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8394)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 8394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SLC26A1_OE_1
FEATURES             Location/Qualifiers
     source          1..8394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1228..1252)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1254..1295
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1337..1925
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1390..1410)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1808..1819)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1997..2230
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2308..2429
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2345..2364)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2399..2418
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2469..2604
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2624..2643)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2625..2643)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2653..2670)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2653..2669)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2662..2684)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2811..3266
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2898..2917)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3108..3129
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3292..3396
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3397..3465,3466..4257)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3615..3634)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4428..5016
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4917..4936
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5170..5187
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5304..5325
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5340..5345,5346..5363,5364..5370)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5378..5394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5383..5405
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5402..5418
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5402..5418
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5437..5457
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5439..5457
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5485..5711
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5712..5892
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5939..6064
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6557..6790
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6975..7019
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7194..7694
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     7613..7631
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             7706..8101
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8161..8278
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatggac gagtcccctg agcctctgca gcagggcaga gggccggtgc
      601 cggtccgacg gcagcgccca gcaccccggg gtctgcgtga gatgctgaag gccaggctgt
      661 ggtgcagctg ctcgtgcagt gtgctgtgcg tccgggcgct ggtgcaggac ctgctccccg
      721 ccacgcgctg gctgcgtcag taccgcccgc gggagtacct ggcaggcgac gtcatgtctg
      781 ggctggtcat cggcatcatc ctggtgccgc aggccatcgc ctactcattg ctggccgggc
      841 tgcagcccat ctacagcctc tatacgtcct tcttcgccaa cctcatctac ttcctcatgg
      901 gcacctcacg gcatgtctcc gtgggcatct tcagcctgct ttgcctcatg gtggggcagg
      961 tggtggaccg ggagctccag ctggccggct ttgacccctc ccaggacggc ctgcagcccg
     1021 gagccaacag cagcaccctc aacggctcgg ctgccatgct ggactgcggg cgtgactgct
     1081 acgccatccg tgtcgccacc gccctcacgc tgatgaccgg gctttaccag acgtcctggg
     1141 gtagaaacag cttccagcaa cacccatggc aattaacaca gaggagtgac tcgcaggaac
     1201 ttctggaaga ggaggaaaga tcttgctgcc caactttctt gtacaaagtg gttggtaagc
     1261 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt
     1321 ggcgcgttaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     1381 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     1441 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     1501 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     1561 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     1621 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     1681 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     1741 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     1801 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     1861 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     1921 cccgcgtcga ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     1981 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt
     2041 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2101 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2161 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2221 atctctagca gtacgtatag tagttcatgt catcttatta ttcagtattt ataacttgca
     2281 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     2341 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     2401 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     2461 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     2521 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     2581 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     2641 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2701 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2761 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     2821 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2881 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2941 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3001 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     3061 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3121 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     3181 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3241 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     3301 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     3361 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     3421 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     3481 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     3541 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     3601 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     3661 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     3721 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     3781 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     3841 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     3901 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     3961 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     4021 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     4081 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     4141 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     4201 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     4261 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4321 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4381 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4441 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4501 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     4561 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     4621 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     4681 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     4741 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     4801 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     4861 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     4921 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     4981 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5041 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5101 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     5161 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     5221 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     5281 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     5341 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     5401 acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa
     5461 caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca
     5521 tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatgccgat
     5581 tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat
     5641 ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc
     5701 gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5761 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5821 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5881 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     5941 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     6001 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     6061 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     6121 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     6181 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     6241 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     6301 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     6361 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     6421 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6481 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6541 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6601 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6661 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6721 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     6781 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     6841 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     6901 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     6961 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     7021 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     7081 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     7141 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccttgcgcc
     7201 ttttccaagg cagccctggg tttgcgcagg gacgcggctg ctctgggcgt ggttccggga
     7261 aacgcagcgg cgccgaccct gggtctcgca cattcttcac gtccgttcgc agcgtcaccc
     7321 ggatcttcgc cgctaccctt gtgggccccc cggcgacgct tcctgctccg cccctaagtc
     7381 gggaaggttc cttgcggttc gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca
     7441 ctagtaccct cgcagacgga cagcgccagg gagcaatggc agcgcgccga ccgcgatggg
     7501 ctgtggccaa tagcggctgc tcagcagggc gcgccgagag cagcggccgg gaaggggcgg
     7561 tgcgggaggc ggggtgtggg gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg
     7621 cattctgcaa gcctccggag cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg
     7681 acctctctcc ccagggggta ccaccatggc caagcctttg tctcaagaag aatccaccct
     7741 cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact acagcgtcgc
     7801 cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat atcattttac
     7861 tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg cagctggcaa
     7921 cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc cctgcggacg
     7981 gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga aggacagtga
     8041 tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg tgtgggaggg
     8101 cctgcagctg cagtagtaag aattctagat cttgagacaa atggcagtat tcatccacaa
     8161 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     8221 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     8281 ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggggg cccggggtta
     8341 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccg
//
