LOCUS       sequence-319560-        9780 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CLCNKA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9780)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CLCNKA_OE_1
FEATURES             Location/Qualifiers
     source          1..9780
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2614..2638)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2640..2681
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2723..3311
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2776..2796)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3194..3205)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3383..3616
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3694..3815
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3731..3750)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3785..3804
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3855..3990
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4010..4029)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4011..4029)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4039..4056)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4039..4055)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4048..4070)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4197..4652
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4284..4303)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4494..4515
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4678..4782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4783..4851,4852..5643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5001..5020)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5814..6402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6303..6322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6556..6573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6690..6711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6726..6731,6732..6749,6750..6756)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6764..6780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6769..6791
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6788..6804
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6788..6804
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6823..6843
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6825..6843
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6871..7097
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7098..7278
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7325..7450
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7943..8176
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8361..8405
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8580..9080
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8999..9017
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9092..9487
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9547..9664
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggcaccatg gaggagttgg tggggctgcg tgagggcttc tcaggggacc
      601 ctgtgactct gcaggagctg tggggcccct gtccccacat ccgccgagcc atccaaggtg
      661 gcctggagtg gctaaagcag aaggtgttcc gcctgggaga agactggtac ttcctgatga
      721 ccctcggggt gctcatggcc ctggtcagct atgccatgaa ctttgccatc gggtgtgtgg
      781 tccgagcaca ccagtggctg tacagggaga ttggggacag ccacctgctc cggtatcttt
      841 cctggactgt gtaccctgtg gccctcgtct ctttctcctc aggcttctcc cagagcatca
      901 cgccctcctc tggaggttct ggaatcccgg agctgaagac catgttggcg ggtgtgatct
      961 tggaggacta cctggatatc aagaactttg gggccaaggt ggtgggcctc tcctgcaccc
     1021 tggccaccgg cagcaccctg ttcctgggca aagtgggccc tttcgtgcac ctgtctgtaa
     1081 tgatcgctgc ctacctgggc cgtgtgcgca ccacgaccat cggggagcct gagaacaaga
     1141 gcaagcaaaa cgaaatgctg gtggcagcgg cggcagtggg cgtggccaca gtctttgcag
     1201 ctcccttcag cggcgtcctg ttcagcatcg aggtcatgtc ttcccacttc tctgtccggg
     1261 attactggag gggcttcttt gcggccacct gcggggcctt catattccgg ctcctggcag
     1321 tcttcaacag cgagcaggag accatcacct ccctctacaa gaccagtttc cgggtggacg
     1381 ttcccttcga cctgcctgag atcttctttt ttgtggcgct gggtggcatc tgcggcgtcc
     1441 tgagctgtgc ttacctcttc tgtcagcgaa ccttcctcag cttcatcaag accaatcggt
     1501 tcagctccaa actgctggct actagcaagc ctgtgtactc cgctctggcc accttgcttc
     1561 tcgcctccat cacctacccg cctggtgtgg gccacttcct agcttctcgg ctgtccatga
     1621 agcagcatct ggactcgctg ttcgacaacc actcctgggc gctgatgacc cagaactcca
     1681 gcccaccctg gcccgaggag ctcgaccccc agcacctttg gtgggaatgg taccacccgc
     1741 ggttcaccat ctttgggacc cttgccttct tcctggttat gaagttctgg atgctgattc
     1801 tggccaccac catccccatg cctgccgggt acttcatgcc catctttatc cttggagctg
     1861 ccatcgggcg cctcttggga gaggctcttg ccgtcgcctt ccctgagggc attgtgactg
     1921 gaggggttac caatcccatc atgcccgggg ggtatgctct ggcaggggct gcagccttct
     1981 caggggctgt gacccacacc atctccacgg cgctgctggc ctttgagctg accggccaga
     2041 tagtgcatgc actgcccgtg ctgatggcgg tgctggcagc caacgccatt gcacagagct
     2101 gccagccctc cttctatgat ggcaccatca ttgtcaagaa gctgccatac ctgccacgga
     2161 ttctgggccg caacatcggc tcccaccatg tgagggtgga gcacttcatg aaccacagca
     2221 tcaccacact ggccaaggac acgccgctgg aggaggtggt caaggttgtg acctccacag
     2281 acgtgaccga gtatcccctg gtggagagca cagagtccca gatcctggta ggcatcgtgc
     2341 agagggccca gctggtgcag gccctccagg ctgagcctcc ttccagggct ccaggacacc
     2401 agtgtctcca ggacatcttg gccaggggct gccccacgga accagtgacc ctgacgctat
     2461 tctcagagac caccttgcac caggcacaaa acctctttaa gctgttgaac cttcagtccc
     2521 tcttcgtgac atcgcggggc agagctgtgg gctgcgtgtc ctgggtggag atgaagaaag
     2581 caatttccaa cctgacaaat ccgccagctc caaagacagc tttcttgtac aaagtggttg
     2641 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
     2701 accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     2761 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2821 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2881 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2941 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     3001 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     3061 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     3121 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     3181 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     3241 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     3301 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     3361 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     3421 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3481 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3541 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3601 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     3661 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3721 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3781 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3841 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3901 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3961 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     4021 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     4081 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     4141 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4201 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4261 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4321 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4381 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4441 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4501 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4561 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4621 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4861 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4921 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4981 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     5041 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5101 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5161 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5221 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5281 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5341 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5401 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5461 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5521 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5581 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5641 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5701 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5941 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6601 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6661 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6721 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6781 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     6841 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     6901 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     6961 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     7021 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     7081 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7141 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7201 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7261 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     7321 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     7381 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     7441 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7501 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7561 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7621 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7681 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7741 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     7801 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     7861 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     7921 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     7981 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     8041 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     8101 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     8161 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     8221 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     8281 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     8341 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     8401 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     8461 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     8521 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct
     8581 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     8641 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     8701 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     8761 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     8821 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     8881 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     8941 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     9001 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     9061 tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc aagaagaatc
     9121 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     9181 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     9241 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     9301 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     9361 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     9421 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     9481 ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg cagtattcat
     9541 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9601 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9661 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg
     9721 gggttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
//
