LOCUS       sequence-319561-        9012 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNK12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9012)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNK12_OE_1
FEATURES             Location/Qualifiers
     source          1..9012
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1846..1870)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1872..1913
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1955..2543
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2008..2028)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2426..2437)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2615..2848
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2926..3047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2963..2982)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3017..3036
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3087..3222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3242..3261)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3243..3261)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3271..3288)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3271..3287)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3280..3302)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3429..3884
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3516..3535)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3726..3747
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3910..4014
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4015..4083,4084..4875)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4233..4252)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5046..5634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5535..5554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5788..5805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5922..5943
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5958..5963,5964..5981,5982..5988)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5996..6012
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6001..6023
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6020..6036
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6020..6036
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6055..6075
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6057..6075
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6103..6329
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6330..6510
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6557..6682
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7175..7408
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7593..7637
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7812..8312
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8231..8249
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8324..8719
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8779..8896
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggcaccatg tcctcccgca gcccccggcc cccgccccgc cgtagccgcc
      601 gccgcctgcc gcgcccctcc tgctgctgct gctgctgccg ccgttcgcac ctcaacgagg
      661 acaccggccg cttcgtgctg ctggcggcgc tcatcggcct ctacctggtg gcgggtgcca
      721 cagtcttctc ggcgctcgag agccccggcg aggcggaggc gcgggcgcgc tggggcgcca
      781 cgctgcgcaa cttcagcgct gcgcacggcg tggccgagcc agagctgcgc gccttcctcc
      841 ggcactacga ggccgcgctg gccgccggcg tccgcgccga cgcgctgcgc ccgcgctggg
      901 acttccccgg cgccttctac ttcgtgggca ccgtggtgtc aaccataggt ttcggcatga
      961 ccacccccgc gacggtgggc gggaaggcct tcctcatcgc ctacgggctg ttcggctgcg
     1021 ctgggaccat cctgttcttc aacctcttcc tggagcgcat catctcgctg ctggccttca
     1081 tcatgcgcgc ctgccgggag cgccagctgc gccgcagcgg cctgctgccc gccaccttcc
     1141 gccgcggctc cgcgctctcg gaggccgaca gcctggcggg ctggaagccc tcggtgtacc
     1201 acgtgctgct catcctgggc ctgttcgccg tgctgctgtc ctgctgcgcc tcggccatgt
     1261 acaccagcgt ggagggctgg gactacgtgg actcgctcta cttctgcttc gtcaccttca
     1321 gcaccatcgg cttcggggac ctggtgagca gccagcacgc cgcctaccgg aaccaggggc
     1381 tctaccgcct gggcaacttc ctcttcatcc tgctcggcgt gtgctgcatt tactcgctct
     1441 tcaacgtcat ctccatcctc atcaagcagg tgctcaactg gatgctgcgc aagctgagct
     1501 gccgctgctg cgcgcgctgc tgcccggctc ctggcgcgcc cctggcccgg cgcaatgcca
     1561 tcaccccagg ctcccggctg cgccgccgcc tggccgcgct cggcgccgac cccgcggccc
     1621 gcgacagcga cgccgagggc cgccgcctct cgggcgagct catctccatg cgcgacctca
     1681 cggcctccaa caaggtgtcg ctggcgctgc tgcagaagca gctgtcggag acggccaacg
     1741 gctacccgcg cagcgtgtgc gtcaacacgc gccagaacgg cttctcgggc ggcgtgggcg
     1801 cgctgggcat catgaacaac cggctggccg agaccagcgc ctccaggaca gctttcttgt
     1861 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     1921 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     1981 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2041 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2101 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2161 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2221 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2281 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2341 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2401 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2461 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2521 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     2581 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     2641 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     2701 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     2761 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     2821 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     2881 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     2941 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3001 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     3061 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     3121 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     3181 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     3241 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     3301 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     3361 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     3421 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     3481 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     3541 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     3601 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     3661 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     3721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     3781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     3841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     3901 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     3961 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     4681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     4741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     4801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     4861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     4921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     4981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     5641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     5701 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     5761 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     5821 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     5881 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     5941 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6001 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     6061 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     6121 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     6181 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     6241 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     6301 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     6361 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     6421 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     6481 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     6541 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     6601 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     6661 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     6721 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     6781 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     6841 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     6901 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     6961 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     7021 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     7081 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7141 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7201 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7261 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7321 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7381 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7441 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7501 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7561 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7621 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7681 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7741 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     7801 cgaggggacc cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     7861 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     7921 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     7981 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     8041 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     8101 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     8161 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     8221 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     8281 tcgttgaccg aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc
     8341 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     8401 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     8461 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     8521 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     8581 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     8641 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
     8701 tggttatgtg tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat
     8761 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     8821 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     8881 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
     8941 cgagggggcc cggggttatt aatagtaatc aattacgggg tcattagttc atagcccata
     9001 tatggagttc cg
//
