LOCUS       sequence-319562-        8847 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNJ15.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8847)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 8847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNJ15_OE_1
FEATURES             Location/Qualifiers
     source          1..8847
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1681..1705)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1707..1748
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1790..2378
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1843..1863)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2261..2272)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2450..2683
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2761..2882
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2798..2817)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2852..2871
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2922..3057
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3077..3096)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3078..3096)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3106..3123)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3106..3122)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3115..3137)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3264..3719
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3351..3370)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3561..3582
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3745..3849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3850..3918,3919..4710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4068..4087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4881..5469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5370..5389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5623..5640
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5757..5778
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5793..5798,5799..5816,5817..5823)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5831..5847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5836..5858
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5855..5871
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5855..5871
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5890..5910
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5892..5910
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5938..6164
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6165..6345
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6392..6517
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7010..7243
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7428..7472
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7647..8147
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8066..8084
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8159..8554
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8614..8731
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatggat gccattcaca tcggcatgtc cagcaccccc ctggtgaagc
      601 acactgctgg ggctgggctc aaggccaaca gaccccgcgt catgtccaag agtgggcaca
      661 gcaacgtgag aattgacaaa gtggatggca tatacctact ctacctgcaa gacctgtgga
      721 ccacagttat cgacatgaag tggagataca aactcaccct gttcgctgcc acttttgtga
      781 tgacctggtt cctttttgga gtcatctact atgccatcgc gtttattcat ggggacttag
      841 aacccggtga gcccatttca aatcataccc cctgcatcat gaaagtggac tctctcactg
      901 gggcgtttct cttttccctg gaatcccaga caaccattgg ctatggagtc cgttccatca
      961 cagaggaatg tcctcatgcc atcttcctgt tggttgctca gttggtcatc acgaccttga
     1021 ttgagatctt catcaccgga accttcctgg ccaaaatcgc cagacccaaa aagcgggctg
     1081 agaccatcaa gttcagccac tgtgcagtca tcaccaagca gaatgggaag ctgtgcttgg
     1141 tgattcaggt agccaatatg aggaagagcc tcttgattca gtgccagctc tctggcaagc
     1201 tcctgcagac ccacgtcacc aaggaggggg agcggattct cctcaaccaa gccactgtca
     1261 aattccacgt ggactcctcc tctgagagcc ccttcctcat tctgcccatg acattctacc
     1321 atgtgctgga tgagacgagc cccctgagag acctcacacc ccaaaaccta aaggagaagg
     1381 agtttgagct tgtggtcctc ctcaatgcca ctgtggaatc caccagcgct gtctgccaga
     1441 gccgaacatc ttatatccca gaggaaatct actggggttt tgagtttgtg cctgtggtat
     1501 ctctctccaa aaatggaaaa tatgtggctg atttcagtca gtttgaacag attcggaaaa
     1561 gcccagattg cacattttac tgtgcagatt ctgagaaaca gcaactcgag gagaagtaca
     1621 ggcaggagga tcagagggaa agagaactga ggacactttt attacaacag agcaatgtct
     1681 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
     1741 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
     1801 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     1861 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     1921 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     1981 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2041 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2101 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2161 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2221 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2281 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2341 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     2401 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2461 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     2521 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2581 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2641 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     2701 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     2761 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     2821 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     2881 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     2941 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3001 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3061 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     3121 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3181 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3241 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3301 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3361 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3421 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3481 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3541 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3601 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3661 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     3721 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     3781 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     3841 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     3901 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     3961 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4021 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4081 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4141 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4201 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4261 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4321 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4381 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4441 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4501 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4561 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4621 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4681 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4741 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     4801 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     4861 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     4921 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     4981 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5041 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5101 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5161 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5221 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5281 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5341 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5401 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5461 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5521 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5581 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5641 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5701 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     5761 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     5821 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     5881 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     5941 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     6001 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     6061 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     6121 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     6181 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6241 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6301 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     6361 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     6421 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     6481 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     6541 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     6601 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     6661 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     6721 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     6781 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     6841 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     6901 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     6961 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7021 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7081 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7141 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7201 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7261 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     7321 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     7381 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     7441 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     7501 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     7561 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     7621 cccacctccc aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc
     7681 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     7741 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     7801 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     7861 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     7921 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     7981 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     8041 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     8101 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccat
     8161 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     8221 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     8281 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     8341 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     8401 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     8461 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     8521 tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aagaattcta
     8581 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     8641 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8701 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     8761 actttggcgc cggctcgagg gggcccgggg ttattaatag taatcaatta cggggtcatt
     8821 agttcatagc ccatatatgg agttccg
//
