LOCUS       sequence-319564-        9087 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses GPR89A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9087)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GPR89A_OE_1
FEATURES             Location/Qualifiers
     source          1..9087
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1921..1945)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1947..1988
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2030..2618
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2083..2103)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2501..2512)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2690..2923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3001..3122
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3038..3057)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3092..3111
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3162..3297
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3317..3336)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3318..3336)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3346..3363)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3346..3362)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3355..3377)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3504..3959
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3591..3610)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3801..3822
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3985..4089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4090..4158,4159..4950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4308..4327)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5121..5709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5610..5629
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5863..5880
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5997..6018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6033..6038,6039..6056,6057..6063)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6071..6087
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6076..6098
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6095..6111
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6095..6111
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6130..6150
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6132..6150
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6178..6404
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6405..6585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6632..6757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7250..7483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7668..7712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7887..8387
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8306..8324
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8399..8794
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8854..8971
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatgagt ttcctcatcg actccagcat catgattacc tcccagatac
      601 tattttttgg atttgggtgg cttttcttca tgcgccaatt gtttaaagac tatgagatac
      661 gtcagtatgt tgtacaggtg atcttctccg tgacgtttgc attttcttgc accatgtttg
      721 agctcatcat ctttgaaatc ttaggagtat tgaatagcag ctcccgttat tttcactgga
      781 aaatgaacct gtgtgtaatt ctgctgatcc tggttttcat ggtgcctttt tacattggct
      841 attttattgt gagcaatatc cgactactgc ataaacaacg actgcttttt tcctgtctct
      901 tatggctgac ctttatgtat ttcttctgga aactaggaga tccctttccc attctcagcc
      961 caaaacatgg gatcttatcc atagaacagc tcatcagccg ggttggtgtg attggagtga
     1021 ctctcatggc tcttctttct ggatttggtg ctgtcaactg cccatacact tacatgtctt
     1081 acttcctcag gaatgtgact gacacggata ttctagccct ggaacggcga ctgctgcaaa
     1141 ccatggatat gatcataagc aaaaagaaaa ggatggcaat ggcacggaga acaatgttcc
     1201 agaaggggga agtgcataac aaaccatcag gtttctgggg aatgataaaa agtgttacca
     1261 cttcagcatc aggaagtgaa aatcttactc ttattcaaca ggaagtggat gctttggaag
     1321 aattaagcag gcagcttttt ctggaaacag ctgatctata tgctaccaag gagagaatag
     1381 aatactccaa aaccttcaag gggaaatatt ttaattttct tggttacttt ttctctattt
     1441 actgtgtttg gaaaattttc atggctacca tcaatattgt ttttgatcga gttgggaaaa
     1501 cggatcctgt cacaagaggc attgagatca ctgtgaatta tctgggaatc caatttgatg
     1561 tgaagttttg gtcccaacac atttccttca ttcttgttgg aataatcatc gtcacatcca
     1621 tcagaggatt gctgatcact cttaccaagt tcttttatgc catctctagc agtaagtcct
     1681 ccaatgtcat tgtcctgcta ttagcacaga taatgggcat gtactttgtc tcctctgtgc
     1741 tgctgatccg aatgagtatg cctttagaat accgcaccat aatcactgaa gtccttggag
     1801 aactgcagtt caacttctat caccgttggt ttgatgtgat cttcctggtc agcgctctct
     1861 ctagcatact cttcctctat ttggctcaca aacaggcacc agagaagcaa atggcacctt
     1921 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
     1981 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
     2041 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     2101 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     2161 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     2221 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     2281 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     2341 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     2401 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     2461 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     2521 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     2581 tcagacgagt cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact
     2641 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     2701 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     2761 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     2821 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     2881 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca
     2941 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     3001 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     3061 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     3121 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     3181 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3241 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     3301 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     3361 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     3421 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     3481 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     3541 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     3601 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     3661 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     3721 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     3781 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     3841 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     3901 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     3961 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4021 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4081 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4141 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4201 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4261 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4321 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4381 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4441 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4501 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4561 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4621 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4681 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4741 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4801 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     4861 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     4921 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     4981 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5041 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5101 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5161 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5221 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     5281 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5341 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5401 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5461 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5521 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5581 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5641 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5701 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5761 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     5821 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     5881 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     5941 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     6001 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     6061 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     6121 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     6181 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     6241 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     6301 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     6361 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     6421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     6601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     6661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     6721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     6781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     6841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     6901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     6961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     7021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     7081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     7141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     7201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     7261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     7321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     7381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     7441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     7501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     7561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     7621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     7681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     7741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     7801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     7861 cccacctccc aaccccgagg ggacccttgc gccttttcca aggcagccct gggtttgcgc
     7921 agggacgcgg ctgctctggg cgtggttccg ggaaacgcag cggcgccgac cctgggtctc
     7981 gcacattctt cacgtccgtt cgcagcgtca cccggatctt cgccgctacc cttgtgggcc
     8041 ccccggcgac gcttcctgct ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt
     8101 gccggacgtg acaaacggaa gccgcacgtc tcactagtac cctcgcagac ggacagcgcc
     8161 agggagcaat ggcagcgcgc cgaccgcgat gggctgtggc caatagcggc tgctcagcag
     8221 ggcgcgccga gagcagcggc cgggaagggg cggtgcggga ggcggggtgt ggggcggtag
     8281 tgtgggccct gttcctgccc gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt
     8341 cggcagtcgg ctccctcgtt gaccgaatca ccgacctctc tccccagggg gtaccaccat
     8401 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     8461 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     8521 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     8581 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     8641 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     8701 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     8761 tgaattgctg ccctctggtt atgtgtggga gggcctgcag ctgcagtagt aagaattcta
     8821 gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg
     8881 gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt
     8941 acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc
     9001 actttggcgc cggctcgagg gggcccgggg ttattaatag taatcaatta cggggtcatt
     9061 agttcatagc ccatatatgg agttccg
//
