LOCUS       sequence-319585-       10551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CLCA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10551)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 10551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CLCA2_OE_1
FEATURES             Location/Qualifiers
     source          1..10551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3385..3409)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3411..3452
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3494..4082
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3547..3567)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3965..3976)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4154..4387
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4465..4586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4502..4521)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4556..4575
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4626..4761
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4781..4800)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4782..4800)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4810..4827)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4810..4826)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4819..4841)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4968..5423
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5055..5074)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5265..5286
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5449..5553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5554..5622,5623..6414)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5772..5791)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6585..7173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7074..7093
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7327..7344
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7461..7482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7497..7502,7503..7520,7521..7527)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7535..7551
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7540..7562
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7559..7575
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7559..7575
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7594..7614
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7596..7614
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7642..7868
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7869..8049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8096..8221
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8714..8947
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9132..9176
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9351..9851
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9770..9788
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9863..10258
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10318..10435
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatgacc caaaggagca ttgcaggtcc tatttgcaac ctgaagtttg
      601 tgactctcct ggttgcctta agttcagaac tcccattcct gggagctgga gtacagcttc
      661 aagacaatgg gtataatgga ttgctcattg caattaatcc tcaggtacct gagaatcaga
      721 acctcatctc aaacattaag gaaatgataa ctgaagcttc attttaccta tttaatgcta
      781 ccaagagaag agtatttttc agaaatataa agattttaat acctgccaca tggaaagcta
      841 ataataacag caaaataaaa caagaatcat atgaaaaggc aaatgtcata gtgactgact
      901 ggtatggggc acatggagat gatccataca ccctacaata cagagggtgt ggaaaagagg
      961 gaaaatacat tcatttcaca cctaatttcc tactgaatga taacttaaca gctggctacg
     1021 gatcacgagg ccgagtgttt gtccatgaat gggcccacct ccgttggggt gtgttcgatg
     1081 agtataacaa tgacaaacct ttctacataa atgggcaaaa tcaaattaaa gtgacaaggt
     1141 gttcatctga catcacaggc atttttgtgt gtgaaaaagg tccttgcccc caagaaaact
     1201 gtattattag taagcttttt aaagaaggat gcacctttat ctacaatagc acccaaaatg
     1261 caactgcatc aataatgttc atgcaaagtt tatcttctgt ggttgaattt tgtaatgcaa
     1321 gtacccacaa ccaagaagca ccaaacctac agaaccagat gtgcagcctc agaagtgcat
     1381 gggatgtaat cacagactct gctgactttc accacagctt tcccatgaat gggactgagc
     1441 ttccacctcc tcccacattc tcgcttgtac aggctggtga caaagtggtc tgtttagtgc
     1501 tggatgtgtc cagcaagatg gcagaggctg acagactcct tcaactacaa caagccgcag
     1561 aattttattt gatgcagatt gttgaaattc ataccttcgt gggcattgcc agtttcgaca
     1621 gcaaaggaga gatcagagcc cagctacacc aaattaacag caatgatgat cgaaagttgc
     1681 tggtttcata tctgcccacc actgtatcag ctaaaacaga catcagcatt tgttcagggc
     1741 ttaagaaagg atttgaggtg gttgaaaaac tgaatggaaa agcttatggc tctgtgatga
     1801 tattagtgac cagcggagat gataagcttc ttggcaattg cttacccact gtgctcagca
     1861 gtggttcaac aattcactcc attgccctgg gttcatctgc agccccaaat ctggaggaat
     1921 tatcacgtct tacaggaggt ttaaagttct ttgttccaga tatatcaaac tccaatagca
     1981 tgattgatgc tttcagtaga atttcctctg gaactggaga cattttccag caacatattc
     2041 agcttgaaag tacaggtgaa aatgtcaaac ctcaccatca attgaaaaac acagtgactg
     2101 tggataatac tgtgggcaac gacactatgt ttctagttac gtggcaggcc agtggtcctc
     2161 ctgagattat attatttgat cctgatggac gaaaatacta cacaaataat tttatcacca
     2221 atctaacttt tcggacagct agtctttgga ttccaggaac agctaagcct gggcactgga
     2281 cttacaccct gaacaatacc catcattctc tgcaagccct gaaagtgaca gtgacctctc
     2341 gcgcctccaa ctcagctgtg cccccagcca ctgtggaagc ctttgtggaa agagacagcc
     2401 tccattttcc tcatcctgtg atgatttatg ccaatgtgaa acagggattt tatcccattc
     2461 ttaatgccac tgtcactgcc acagttgagc cagagactgg agatcctgtt acgctgagac
     2521 tccttgatga tggagcaggt gctgatgtta taaaaaatga tggaatttac tcgaggtatt
     2581 ttttctcctt tgctgcaaat ggtagatata gcttgaaagt gcatgtcaat cactctccca
     2641 gcataagcac cccagcccac tctattccag ggagtcatgc tatgtatgta ccaggttaca
     2701 cagcaaacgg taatattcag atgaatgctc caaggaaatc agtaggcaga aatgaggagg
     2761 agcgaaagtg gggctttagc cgagtcagct caggaggctc cttttcagtg ctgggagttc
     2821 cagctggccc ccaccctgat gtgtttccac catgcaaaat tattgacctg gaagctgtaa
     2881 aagtagaaga ggaattgacc ctatcttgga cagcacctgg agaagacttt gatcagggcc
     2941 aggctacaag ctatgaaata agaatgagta aaagtctaca gaatatccaa gatgacttta
     3001 acaatgctat tttagtaaat acatcaaagc gaaatcctca gcaagctggc atcagggaga
     3061 tatttacgtt ctcaccccaa atttccacga atggacctga acatcagcca aatggagaaa
     3121 cacatgaaag ccacagaatt tatgttgcaa tacgagcaat ggataggaac tccttacagt
     3181 ctgctgtatc taacattgcc caggcgcctc tgtttattcc ccccaattct gatcctgtac
     3241 ctgccagaga ttatcttata ttgaaaggag ttttaacagc aatgggtttg ataggaatca
     3301 tttgccttat tatagttgtg acacatcata ctttaagcag gaaaaagaga gcagacaaga
     3361 aagagaatgg aacaaaatta ttatacccaa ctttcttgta caaagtggtt ggtaagccta
     3421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
     3481 gcgttaagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     3541 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     3601 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     3661 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     3721 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     3781 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     3841 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     3901 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     3961 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     4021 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     4081 gcgtcgactt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     4141 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     4201 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     4261 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     4321 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     4381 tctagcagta cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     4441 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     4501 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     4561 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     4621 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     4681 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     4741 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     4801 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     4861 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     4921 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     4981 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5041 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5101 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5161 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5221 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5281 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5341 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5401 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     5461 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5521 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5581 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5641 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5701 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5761 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5821 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5881 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5941 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6001 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6061 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6121 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6181 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6241 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6301 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6361 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6421 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6481 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6541 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6601 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6661 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6721 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6781 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6841 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6901 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6961 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7021 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7081 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7141 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7201 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7261 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7321 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7381 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     7441 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7501 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7561 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     7621 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     7681 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg
     7741 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     7801 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     7861 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7921 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7981 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     8041 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     8101 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     8161 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     8221 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     8281 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     8341 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     8401 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     8461 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     8521 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     8581 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     8641 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     8701 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     8761 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     8821 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     8881 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     8941 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     9001 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     9061 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     9121 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     9181 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     9241 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     9301 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc ttgcgccttt
     9361 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     9421 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     9481 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     9541 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     9601 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     9661 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     9721 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     9781 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     9841 tctctcccca gggggtacca ccatggccaa gcctttgtct caagaagaat ccaccctcat
     9901 tgaaagagca acggctacaa tcaacagcat ccccatctct gaagactaca gcgtcgccag
     9961 cgcagctctc tctagcgacg gccgcatctt cactggtgtc aatgtatatc attttactgg
    10021 gggaccttgt gcagaactcg tggtgctggg cactgctgct gctgcggcag ctggcaacct
    10081 gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc ttgagcccct gcggacggtg
    10141 ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc atagtgaagg acagtgatgg
    10201 acagccgacg gcagttggga ttcgtgaatt gctgccctct ggttatgtgt gggagggcct
    10261 gcagctgcag tagtaagaat tctagatctt gagacaaatg gcagtattca tccacaattt
    10321 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    10381 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    10441 ttattacagg gacagcagag atccactttg gcgccggctc gagggggccc ggggttatta
    10501 atagtaatca attacggggt cattagttca tagcccatat atggagttcc g
//
