LOCUS       sequence-319587-        8826 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNAB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8826)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 8826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNAB2_OE_1
FEATURES             Location/Qualifiers
     source          1..8826
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1660..1684)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1686..1727
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1769..2357
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1822..1842)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2240..2251)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2429..2662
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2740..2861
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2777..2796)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2831..2850
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2901..3036
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3056..3075)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3057..3075)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3085..3102)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3085..3101)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3094..3116)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3243..3698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3330..3349)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3540..3561
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3724..3828
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3829..3897,3898..4689)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4047..4066)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4860..5448
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5349..5368
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5602..5619
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5736..5757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5772..5777,5778..5795,5796..5802)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5810..5826
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5815..5837
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5834..5850
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5834..5850
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5869..5889
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5871..5889
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5917..6143
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6144..6324
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6371..6496
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6989..7222
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7407..7451
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7626..8126
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8045..8063
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8138..8533
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8593..8710
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcaccatg tatccagaat caacgacggg ctccccggct cggctctcgc
      601 tgcggcagac gggctccccc gggatgatct acagtactcg gtatgggagt cccaaaagac
      661 agctccagtt ttacaggaac ctgggcaagt ctggcctgcg ggtctcctgc ctgggacttg
      721 gaacatgggt gaccttcgga ggccagatca ccgatgagat ggcagagcag ctcatgacct
      781 tggcctatga taatggcatc aacctcttcg atacagcaga agtctacgca gccggcaagg
      841 ctgaagtggt actgggaaac atcattaaga agaaaggatg gaggcggtcc agcctcgtca
      901 tcaccaccaa gatcttctgg ggcggaaagg cggagacgga gcggggcctg tccaggaagc
      961 acataatcga aggtctgaaa gcttccctgg agcgactgca gctggagtac gtggatgtgg
     1021 tgtttgccaa ccgcccggac cccaacaccc cgatggaaga gaccgtccgc gccatgaccc
     1081 acgtcatcaa ccaggggatg gccatgtact ggggcacgtc acgctggagc tccatggaga
     1141 tcatggaggc ctactccgtg gcccggcagt tcaacctgac cccgcccatc tgcgagcagg
     1201 ctgagtacca catgttccag cgtgagaaag tggaggtgca gctgccggag ctgttccaca
     1261 agataggagt gggcgccatg acctggtccc ctctggcctg tggcattgtt tctggcaagt
     1321 acgacagtgg catcccaccc tactcaagag cctccttgaa gggctaccag tggctgaagg
     1381 acaagatcct cagtgaggag ggccggcgcc agcaagccaa gctgaaggag ctgcaggcca
     1441 tcgccgagcg cctgggctgc accctgcccc agctggccat agcctggtgc ctgaggaatg
     1501 agggagtcag ctccgtgctc ctgggggcct ccaatgcgga ccagctcatg gagaacattg
     1561 gggcaataca ggtccttccg aaactgtcgt cttccattat ccacgagatt gatagtattt
     1621 tgggcaataa accctacagc aaaaaggact acagatcctt gccaactttc ttgtacaaag
     1681 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     1741 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     1801 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     1861 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     1921 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     1981 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2041 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2101 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2161 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2221 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2281 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2341 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     2401 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     2461 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2521 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2581 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2641 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     2701 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     2761 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     2821 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     2881 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     2941 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3001 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     3061 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3121 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3181 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3241 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3301 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3361 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3421 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3481 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3541 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3601 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3661 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3721 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     3781 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     3841 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     3901 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     3961 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4021 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4081 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4141 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4201 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4261 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4321 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4381 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4441 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4501 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4561 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4621 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4681 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4741 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     4801 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     4861 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     4921 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     4981 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5041 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5101 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5161 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5221 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5281 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5341 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5401 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5461 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5521 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5581 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5641 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5701 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     5761 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     5821 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     5881 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     5941 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6001 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6061 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6121 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6181 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6241 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6301 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6361 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6421 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6481 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6541 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6601 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     6661 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     6721 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     6781 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     6841 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     6901 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     6961 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7021 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7081 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7141 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7201 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7261 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7321 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7381 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7441 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7501 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7561 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7621 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     7681 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     7741 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     7801 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     7861 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     7921 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     7981 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     8041 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     8101 accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga
     8161 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     8221 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     8281 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     8341 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     8401 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     8461 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     8521 tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt
     8581 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8641 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8701 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     8761 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     8821 gttccg
//
