LOCUS       sequence-319591-       10854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10854)
  TITLE     Immunoprecipitation of the endoplasmic reticulum (ER-IP)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: control (pGS701)
FEATURES             Location/Qualifiers
     source          1..10854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1570..1687
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2786..3972
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3017..3963
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3920..3940
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             4062..4142
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     misc_feature    4909..5461
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5053..5070)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5280..5299
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5470..5865
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             5875..5931
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5941..6486
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             6496..6516
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6523..7111
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6576..6596)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6994..7005)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7497..7730
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7802..7923
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7839..7858)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7893..7912
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8025..8044
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(8309..8327)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8395..8499
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8500..8568,8569..9360)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8718..8737)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9531..10119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10020..10039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    10447..10468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10483..10488,10489..10506,10507..10513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10521..10537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10526..10548
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10545..10561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10545..10561
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10580..10600
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10582..10600
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10628..10854
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1621 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     1681 aaattttatc gataccgtcg agtttactcc ctattctaga caaatgcttg tccttccttc
     1741 ctgtaacaca gccattaaac caggagcatc gcccttcccc ggccctcagg taagaggacc
     1801 aaataccgta gccgtttcca atttcagtcc tttagcgcca cctggtgcta actactctat
     1861 cacgctttta tccaataact acctttgtaa atttcctttc aaaagttctg gccgggcgcg
     1921 gtggctcacg cttgtaatcc cagcactttg tgaggggtca ggagttcgag acattaatag
     1981 ggaggtggtc cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt
     2041 ccctttttgt tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc
     2101 gaatcaactt ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct
     2161 gtggaactga cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac
     2221 tagtttttaa gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac
     2281 cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc
     2341 agaaaagcca ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc
     2401 cccttgggga ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc
     2461 cgccctcccc ctagggcccc aattcccagc gggcgcggcg cgcggcccct ccccccgccg
     2521 ggcgcgcgcc cgctgccccg cccttcgtgg ccgcccggcg tgggcggtgc cacccctccc
     2581 cccggcggcc ccgcgcgcag ctcccggctc cctccccctt cggatgtggc ttgagctgta
     2641 gcgcggagtt taattaaaat cgcgtaccgt cgacctcgac ggatcgggag atctcgaccg
     2701 agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     2761 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     2821 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     2881 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     2941 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     3001 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     3061 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     3121 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     3181 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     3241 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     3301 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatcgatc
     3361 tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc
     3421 gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt
     3481 ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct
     3541 gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg
     3601 gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt
     3661 cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg
     3721 agtaccgggc gccgtccagg cacctcgatt agttctcgat cgagcttttg gagtacgtcg
     3781 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3841 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3901 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3961 caggtgtcgt gaggaattag cttgaccaag cttggtaccg agctcggatc cactagtaac
     4021 ggccgccagt gtgctggaat tatcacaagg ccgccaccat gtacccatac gatgttccag
     4081 attacgctta tccctacgac gtgcctgatt atgcataccc atatgatgtc cccgactatg
     4141 ccggcggatc aagtaagggg gaggagctgt ttacgggtgt agtacccata cttgtcgaac
     4201 tggacggtga tgttaatgga cataagttta gtgtcagagg ggaaggggaa ggggatgcaa
     4261 cgaatggaaa gctgacgctc aagttcatct gtacaactgg caaactcccg gtgccttggc
     4321 caactctcgt aaccacattg acatacggag tccagtgctt tagtaggtat ccggatcata
     4381 tgaagcagca tgatttcttt aagtcagcca tgccagaggg ttatgtccag gaacgaacta
     4441 tcagttttaa ggatgatgga acgtacaaaa ctcgagcaga agtcaagttc gaaggcgata
     4501 ctttggtaaa ccgcatagaa ctcaaaggga tcgacttcaa agaagatgga aacatactgg
     4561 gccataaact ggaatataac tttaactccc acaatgttta catcactgcc gataagcaga
     4621 agaacggtat taaggccaat tttaagatta ggcataatgt agaagatggg tcagttcaat
     4681 tggcggatca ttatcagcaa aacacgccga taggagacgg tcccgtgctt ctcccagaca
     4741 accactatct tagcacacag tctaagctga gtaaggaccc taatgagaag agggatcaca
     4801 tggtacttct ggaatttgtt acggcggcag gtatcaccca cggtatggat gaactctaca
     4861 agggtggtgg ttctggaggc ggcggatcac gcgttgcagt ttaaactaac gttactggcc
     4921 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     4981 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     5041 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     5101 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     5161 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     5221 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     5281 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     5341 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     5401 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     5461 atggccacaa tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     5521 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     5581 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     5641 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     5701 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     5761 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     5821 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcggatc cggcgcaaca
     5881 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gggatccggc
     5941 atggacaaag actgcgaaat gaagcgcacc accctggata gccctctggg caagctggaa
     6001 ctgtctgggt gcgaacaggg cctgcaccgt atcatcttcc tgggcaaagg aacatctgcc
     6061 gccgacgccg tggaagtgcc tgccccagcc gccgtgctgg gcggaccaga gccactgatg
     6121 caggccaccg cctggctcaa cgcctacttt caccagcctg aggccatcga ggagttccct
     6181 gtgccagccc tgcaccaccc agtgttccag caggagagct ttacccgcca ggtgctgtgg
     6241 aaactgctga aagtggtgaa gttcggagag gtcatcagct acagccacct ggccgccctg
     6301 gccggcaatc ccgccgccac cgccgccgtg aaaaccgccc tgagcggaaa tcccgtgccc
     6361 attctgatcc cctgccaccg ggtggtgcag ggcgacctgg acgtgggggg ctacgagggc
     6421 gggctcgccg tgaaagagtg gctgctggcc cacgagggcc acagactggg caagcctggg
     6481 ctgggtggcg ggagcccaaa gaagaagcgg aaggtgtagc ggaatcaacc tctggattac
     6541 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6601 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6661 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6721 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6781 acctgtcagc tcctttccgg aactttcgct ttccccctcc ctattgccac ggcggaactc
     6841 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6901 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctatgt tgccacctgg
     6961 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7021 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7081 agtcggatct ccctttgggc cgcctccccg catcgatacc gagcgctgct cgaggtacgc
     7141 caacagagcg agattccgtc tcaaagaaaa aaaaagtaat gaaatgaata aaatgagtcc
     7201 tagagccagt aaatgtcgta aatgtctcag ctagtcaggt agtaaaaggt ctcaactagg
     7261 cagtggcaga gcaggattca aattcagggc tgttgtgatg cctccgcaga ctctgagcgc
     7321 cacctggtgg taatttgtct gtgcctcttc tgacgttgaa gaacagcaac taacacacta
     7381 acacggcatt tactatgggc cagccattgt tctaatgtta actgtggtac ctttaagacc
     7441 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7501 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7561 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7621 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7681 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7741 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7801 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7921 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     7981 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8041 gaggccggat cccttgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     8101 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     8161 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     8221 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     8281 ccccttgtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     8341 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     8401 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     8461 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     8521 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     8581 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     8641 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     8701 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     8761 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     8821 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     8881 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     8941 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     9001 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     9061 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     9121 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     9181 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     9241 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     9301 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     9361 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     9421 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     9481 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     9541 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     9601 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9661 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9721 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9781 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9841 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9901 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9961 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    10021 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    10081 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    10141 ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc atctacctgc
    10201 ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata
    10261 atggggaagg ccatccagcc tcgcgtcgcg ataaccctaa ttcgatagca tatgcttccc
    10321 gttgggtaac atatgctatt gaattagggt tagtctggat agtatatact actacccggg
    10381 aagcatatgc tacccgttta gggttcaccg gtgatgccgg ccacgatgcg tccggcgtag
    10441 aggatctaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
    10501 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
    10561 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
    10621 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
    10681 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
    10741 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
    10801 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaac
//
