LOCUS       sequence-319595-        9309 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CHRNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9309)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9309)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CHRNA2_OE_1
FEATURES             Location/Qualifiers
     source          1..9309
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2143..2167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2169..2210
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2252..2840
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2305..2325)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2723..2734)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2912..3145
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3223..3344
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3260..3279)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3314..3333
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3384..3519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3539..3558)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3540..3558)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3568..3585)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3568..3584)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3577..3599)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3726..4181
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3813..3832)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4023..4044
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4207..4311
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4312..4380,4381..5172)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4530..4549)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5343..5931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5832..5851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6085..6102
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6219..6240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6255..6260,6261..6278,6279..6285)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6293..6309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6298..6320
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6317..6333
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6317..6333
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6352..6372
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6354..6372
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6400..6626
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6627..6807
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6854..6979
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7472..7705
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7890..7934
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8109..8609
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8528..8546
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8621..9016
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9076..9193
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagc aggcaccatg ggcccctcct gtcctgtgtt cctgtccttc acaaagctca
      601 gcctgtggtg gctccttctg accccagcag gtggagagga agctaagcgc ccacctccca
      661 gggctcctgg agacccactc tcctctccca gtcccacggc attgccgcag ggaggctcgc
      721 ataccgagac tgaggaccgg ctcttcaaac acctcttccg gggctacaac cgctgggcgc
      781 gcccggtgcc caacacttca gacgtggtga ttgtgcgctt tggactgtcc atcgctcagc
      841 tcatcgatgt ggatgagaag aaccaaatga tgaccaccaa cgtctggcta aaacaggagt
      901 ggagcgacta caaactgcgc tggaacccca ctgattttgg caacatcaca tctctcaggg
      961 tcccttctga gatgatctgg atccccgaca ttgttctcta caacaatgca gatggggagt
     1021 ttgcagtgac ccacatgacc aaggcccacc tcttctccac gggcactgtg cactgggtgc
     1081 ccccggccat ctacaagagc tcctgcagca tcgacgtcac cttcttcccc ttcgaccagc
     1141 agaactgcaa gatgaagttt ggctcctgga cttatgacaa ggccaagatc gacctggagc
     1201 agatggagca gactgtggac ctgaaggact actgggagag cggcgagtgg gccatcgtca
     1261 atgccacggg cacctacaac agcaagaagt acgactgctg cgccgagatc taccccgacg
     1321 tcacctacgc cttcgtcatc cggcggctgc cgctcttcta caccatcaac ctcatcatcc
     1381 cctgcctgct catctcctgc ctcactgtgc tggtcttcta cctgccctcc gactgcggcg
     1441 agaagatcac gctgtgcatt tcggtgctgc tgtcactcac cgtcttcctg ctgctcatca
     1501 ctgagatcat cccgtccacc tcgctggtca tcccgctcat cggcgagtac ctgctgttca
     1561 ccatgatctt cgtcaccctg tccatcgtca tcaccgtctt cgtgctcaat gtgcaccacc
     1621 gctcccccag cacccacacc atgccccact gggtgcgggg ggcccttctg ggctgtgtgc
     1681 cccggtggct tctgatgaac cggcccccac cacccgtgga gctctgccac cccctacgcc
     1741 tgaagctcag cccctcttat cactggctgg agagcaacgt ggatgccgag gagagggagg
     1801 tggtggtgga ggaggaggac agatgggcat gtgcaggtca tgtggccccc tctgtgggca
     1861 ccctctgcag ccacggccac ctgcactctg gggcctcagg tcccaaggct gaggctctgc
     1921 tgcaggaggg tgagctgctg ctatcacccc acatgcagaa ggcactggaa ggtgtgcact
     1981 acattgccga ccacctgcgg tctgaggatg ctgactcttc ggtgaaggag gactggaagt
     2041 atgttgccat ggtcatcgac aggatcttcc tctggctgtt tatcatcgtc tgcttcctgg
     2101 ggaccatcgg cctctttctg cctccgttcc tagctggaat gatcacagct ttcttgtaca
     2161 aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
     2221 ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggattaca aaatttgtga
     2281 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     2341 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     2401 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     2461 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     2521 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     2581 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     2641 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     2701 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     2761 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     2821 cctttgggcc gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat
     2881 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     2941 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     3001 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3061 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3121 tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag
     3181 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
     3241 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     3301 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
     3361 ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     3421 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     3481 agctattcca gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc
     3541 ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga
     3601 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     3661 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     3721 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3781 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3841 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3901 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3961 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4021 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4081 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4141 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     4201 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     4261 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     4321 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     4381 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     4441 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     4501 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     4561 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     4621 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     4681 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     4741 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     4801 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     4861 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     4921 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     4981 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     5041 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     5101 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     5161 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     5221 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     5281 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     5341 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     5401 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     5461 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     5521 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     5581 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     5641 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     5701 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     5761 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     5821 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     5881 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     5941 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6001 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6061 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     6121 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     6181 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6241 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6301 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     6361 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     6421 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     6481 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     6541 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     6601 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     6661 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     6721 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     6781 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     6841 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     6901 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     6961 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     7021 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     7081 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     7141 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     7201 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     7261 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     7321 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     7381 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     7441 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     7501 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     7561 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     7621 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     7681 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     7741 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     7801 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     7861 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     7921 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     7981 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     8041 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     8101 ggggaccctt gcgccttttc caaggcagcc ctgggtttgc gcagggacgc ggctgctctg
     8161 ggcgtggttc cgggaaacgc agcggcgccg accctgggtc tcgcacattc ttcacgtccg
     8221 ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg ccccccggcg acgcttcctg
     8281 ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg
     8341 aagccgcacg tctcactagt accctcgcag acggacagcg ccagggagca atggcagcgc
     8401 gccgaccgcg atgggctgtg gccaatagcg gctgctcagc agggcgcgcc gagagcagcg
     8461 gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc
     8521 ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg
     8581 ttgaccgaat caccgacctc tctccccagg gggtaccacc atggccaagc ctttgtctca
     8641 agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga
     8701 agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa
     8761 tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc
     8821 tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt
     8881 gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat
     8941 agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg
     9001 ttatgtgtgg gagggcctgc agctgcagta gtaagaattc tagatcttga gacaaatggc
     9061 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     9121 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     9181 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggctcga
     9241 gggggcccgg ggttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     9301 ggagttccg
//
