LOCUS       sequence-319596-        9066 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses HTR3C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9066)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_HTR3C_OE_1
FEATURES             Location/Qualifiers
     source          1..9066
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1900..1924)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1926..1967
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2009..2597
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2062..2082)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2480..2491)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2669..2902
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2980..3101
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3017..3036)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3071..3090
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3141..3276
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3296..3315)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3297..3315)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3325..3342)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3325..3341)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3334..3356)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3483..3938
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3570..3589)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3780..3801
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3964..4068
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4069..4137,4138..4929)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4287..4306)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5100..5688
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5589..5608
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5842..5859
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5976..5997
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6012..6017,6018..6035,6036..6042)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6050..6066
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6055..6077
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6074..6090
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6074..6090
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6109..6129
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6111..6129
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6157..6383
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6384..6564
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6611..6736
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7229..7462
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7647..7691
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7866..8366
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8285..8303
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8378..8773
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8833..8950
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcaccatg gaaggagggt ggcctgcaag gcagagtgcc ctcctctgcc
      601 tcactgtcag tcttctgctt caaggaagag gcgacgcttt taccatcaat tgctcaggct
      661 ttgaccagca tggggttgac cctgctgtct tccaagcagt gtttgacaga aaggccttcc
      721 gtccattcac caactacagc atccctaccc gtgtcaacat ctccttcacc ctgtctgcca
      781 tcctgggagt ggatgcacag ctccagctgc tgacatcatt cctgtggatg gatttggtat
      841 gggacaatcc tttcattaat tggaacccaa aagagtgtgt tggcatcaat aaactcacag
      901 tattagctga aaacctgtgg ctcccagaca tcttcatcgt ggaatccatg gatgtggatc
      961 agacgccttc cggtctcact gcctatatca gcagtgaagg tcgaattaag tatgataagc
     1021 caatgagggt gaccagcatc tgtaacctgg acatcttcta cttccctttt gaccaacaga
     1081 actgtacctt caccttcagt tctttcctct acacagtgga cagcatgctg ctgggcatgg
     1141 acaaggaggt gtgggagatc acagacacgt ctcgcaaagt catccaaacc cagggggagt
     1201 gggagctctt gggcatcaac aaggccaccc caaagatgtc catgggcaac aacctatatg
     1261 accagatcat gttttatgtg gccatcaggc gcaggccaag cctctacatc ataaacctgc
     1321 tggtgcccag tagctttctg gttgccattg atgccctcag cttctacctg ccagcagaga
     1381 gcgagaatcg tgccccattc aagataacac ttctgctggg ctacaacgtc ttcctgctca
     1441 tgatgaatga cttgctccct gccagtggca cccccctcat cagtgtctac ttcgccctgt
     1501 gcctgtccct gatggtggtc agcctgctgg agaccgtctt cattacctac ctgctgcacg
     1561 tggccaccac ccagccccca cccatgccta ggtggcttca ctccctgctg ctccactgca
     1621 ccagcccagg gagatgctgt cccactgcgc cccagaaggg aaataagggc ctgggtctca
     1681 ccctcaccca cctgcctggc ccaaaggagc cgggggagtt agcagggaag aagctgggac
     1741 ccagagagac cgagccagat gggggctcag gatggacaaa gacccagcta atggagctgt
     1801 gggtgcagtt cagccacgcg atggacaccc tgctcttccg cctctacctg ctcttcatgg
     1861 cctcctccat ccttactgtc attgtcctct ggaacacctt gccaactttc ttgtacaaag
     1921 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
     1981 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     2041 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2101 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2161 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2221 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2281 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2341 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2401 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2461 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2521 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2581 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     2641 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     2701 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     2761 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2821 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2881 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     2941 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     3001 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3061 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     3121 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3181 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3241 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     3301 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     3361 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     3421 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     3481 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     3541 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     3601 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     3661 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     3721 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     3781 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     3841 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     3901 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     3961 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4021 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4081 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4141 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4201 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4261 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4321 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     4381 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     4441 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     4501 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     4561 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     4621 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     4681 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     4741 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     4801 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     4861 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     4921 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     4981 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5041 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5101 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5161 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5221 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5281 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     5341 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     5401 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     5461 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     5521 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     5581 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     5641 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     5701 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     5761 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     5821 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     5881 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     5941 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6001 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6061 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6121 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6181 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6241 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6301 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     6361 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     6421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6541 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     6601 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     6661 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     6721 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     6781 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     6841 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     6901 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     6961 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7021 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7081 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7141 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7201 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7261 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7321 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     7381 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     7441 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     7501 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     7561 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     7621 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat
     7681 gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata aaattattca
     7741 taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga
     7801 atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg
     7861 gacccttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc
     7921 gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc
     7981 gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc
     8041 cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag
     8101 ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc
     8161 gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc
     8221 gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg
     8281 cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg
     8341 accgaatcac cgacctctct ccccaggggg taccaccatg gccaagcctt tgtctcaaga
     8401 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
     8461 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
     8521 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
     8581 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
     8641 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
     8701 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
     8761 tgtgtgggag ggcctgcagc tgcagtagta agaattctag atcttgagac aaatggcagt
     8821 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     8881 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     8941 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggctcgaggg
     9001 ggcccggggt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     9061 gttccg
//
