LOCUS       sequence-319606-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    385..466
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(422..441)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(477..494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcagagctgg tttagtgaac cgtcagatcc gctagggatc ctctagtcag ctgacgcgtg
       61 ctagcgcggc cgcgttctgc ccccagcccc tcacagtgct tttcaagcca tgcttcctgt
      121 gcccccagtg gggccctggc tgggatatca tcatatactg taagtttgcg atgagacact
      181 acagtataga tgatgtacta gtccgggcac ccccagctct ggagcctgac aaggaggaca
      241 ggagagatgc tgcaagcttg tcgacgatat ctccagagga tcataatcag ccataccaca
      301 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
      361 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
      421 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgccccg agcttcctcg
      481 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      541 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      601 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      661 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      721 ggactataaa gataccag
//
