LOCUS       sequence-319608-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..66
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     polyA_signal    427..508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(464..483)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        541..645
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atgtcgaggt ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt
       61 cagatcgcct ggagaattca ccggtcatat gccgcggggg cccagatctg gggtcagctt
      121 ctaccggggc ggcgtcccgg ggtcttggaa ctgcatgcag actgcctgct tgggaaacat
      181 acttctttat atgcccatat ggacctgcta agctatggaa tgtaaagaag tatgtatctc
      241 aggccgggac ctctctcgcc gcactgaggg gcactccaca ccacgggggc cgccgcggcc
      301 gacagccctg cagtctagag gatcataatc agccatacca catttgtaga ggttttactt
      361 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      421 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      481 ttcacaaata aagcattttt ttcactgccg acgtcaggtg gcacttttcg gggaaatgtg
      541 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      601 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      661 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      721 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      781 gaactggatc tcaacagcgg taag
//
