LOCUS       sequence-319622-        9203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast display reporter for non-display yeast strains (wild-type 
            construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Potts KA, Stieglitz JT, Lei M, Van Deventer JA
  TITLE     Reporter system architecture affects measurements of noncanonical 
            amino acid incorporation efficiency and fidelity.
  JOURNAL   Mol Syst Des Eng. 2020 Feb 1;5(2):573-588. doi: 10.1039/c9me00107g. 
            Epub 2020 Jan 23.
  PUBMED    33791108
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c9me00107g"; journalName: "Mol Syst Des Eng"; date: 
            "2020-02-1- 1"; volume: "5"; issue: "2"; pages: "573-588"
COMMENT     Sequence Label: pRS416-Aga1p-FAPB2.3.6
FEATURES             Location/Qualifiers
     source          1..9203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        128..232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(233..301,302..1093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(451..470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1264..1852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1753..1772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2006..2023
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2140..2161
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2176..2181,2182..2199,2200..2206)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2214..2230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2219..2241
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2238..2254
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2238..2254
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2273..2293
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2275..2293
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4531..5183
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    4735..4852
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     5147..5170
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5502..5513
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5514..5540
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6342..6371
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(6738..6757)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6739..6757)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6767..6784)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6767..6783)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6776..6798)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6924..7379
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7011..7030)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7221..7242
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7510..8313)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(8314..8529)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(8553..8572)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8672..8694
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8732..8750)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    join(8791..9203,1..91)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
ORIGIN
        1 tttttcttta atttcttttt ttactttcta tttttaattt atatatttat attaaaaaat
       61 ttaaattata attattttta tagcacgtga tgaaaaggac ccaggtggca cttttcgggg
      121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      181 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      241 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      301 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      361 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      421 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      481 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      541 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      601 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      661 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
      721 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
      781 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
      841 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
      901 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
      961 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     1021 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     1081 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     1141 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     1201 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     1261 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     1321 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     1381 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     1441 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     1501 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     1561 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     1621 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     1681 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     1741 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     1801 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     1861 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     1921 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     1981 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     2041 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
     2101 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     2161 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     2221 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
     2281 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaggtac
     2341 cttaactgaa aattacattg caagcaactg ccatgatggc gaagaccaac tttcctaatg
     2401 gcaaacgcgt ttgcgatcca ctgcccatat attggctaat tgttaccatt gatgaagttt
     2461 ggtattgtat ggagattgaa gtagtggaaa cttctgtagt ggtaacagat gtttgtagag
     2521 aagagggagc agatgatgat gagaggaaaa tagaagatac cgtcggagtc aatgatgaag
     2581 attcaatagt cgattccaca gaggtagatg gtgtagagat agatgttgcg gaaagtgatg
     2641 atgtagaggc agatattgta gcaatagctg tggaagaagt gggttgtaaa atctctgatg
     2701 ttgaagaaat ttgaagtgtg gtaactggtg actgttcgac acttagtgat gatacagaac
     2761 tgaatagcaa aatagtactg tcagaggata atgtagcaga caaggaggag gatgaaagga
     2821 ccgatgtcga taataagtgt gatgattgtt cttgagattc tgagtcaatg cttgatgtac
     2881 tcgtggcttc acttacacta gtcgaaatgg ttgaggtacc tgaagatgtt acatgatact
     2941 taccggtagc gtgcttattg aaatcttcat ccgaagttgt cattgaactt acccaactct
     3001 ttgtggatgt ggtctgctgt gttacagaac ttgatgaagc tgtttgcacg gaagaatacg
     3061 agtgtgacgt ttcagcacat gtggagcatg catatgagga ttcaaccgtt gaggtgctta
     3121 cagaagttgc gacgttttta gtggacattt ttatagaaga tgtaatcaaa cttgttgtct
     3181 gcgagggcat gcaagactcg tgagtgcaga ctgtagcgtc tgttgtaacc gtttcatgca
     3241 tactggttaa tgtgctagtc tcagattcag agctataagg acaccatgtc gtatacattg
     3301 tagtgactcc actaacagtg gtgaaatatg tagacatgga aaatgatggg atagtagtag
     3361 aaattgagga tttggtgata tattcagacg aactttgtga actaacagtt gtttcaacag
     3421 ttgaagaagt catagaagga gttgcaacat ttgacgaagt cgaagggacg gagtaaacag
     3481 gtgtggatgg gctgtataat gacacagacg ttgatgaaga tgctatagag gagccaaggg
     3541 atgaagttga atcagtaaaa gtagagctaa tagatgttga acttggagaa gtggaagcca
     3601 aagttggaga tgaagaagtc aaacttgggg atgtagatgt tgaatatgaa gaagtggaag
     3661 tggagcttgc agaagtagat ttagaacttg gagatgtaga tgttgaactt gatgaggtag
     3721 atgtagagct tggagatgta gatgtcgaac ttgatgaggt agatgtagaa ctttgggatg
     3781 tagatgtaga acttgaagat gtggatgtca aacttgaaga tgtggatgtc gaacttggag
     3841 atgtagatgt cgaacttgat gaggtagatg tcgaacttga tgaggtagat gtcgaacttg
     3901 atgaggtaga tgtagagctt ggagatgtag atgtagagct tggagatgta gatgtcgaac
     3961 ttagggaggt agtagttgga ttggacgatg ttgtactcga aagtgtagag gtgacaggtg
     4021 agattataga ggcacttgat ggttcaatgg cgctggatga taccacggtt gtagttccta
     4081 cttcggataa tgagctgata gcggttgtgt gacaagtagg gcaaatatat gaagtgaact
     4141 tggaagtcac agataaggtg gtggttggca acgtcgatga cgaaggacat gcctcatggg
     4201 agcagacttc tgtagataat gtcaaagtac taaatctgga tagggtagta gcgtacgata
     4261 ttgaaggact tatttcggca gcagatgaag tggataccgt caatggacac caagtcgtat
     4321 acaatgtcgt agtgccagct tgaacgatag tggatgtgga gactagcgcg ggtgaaactg
     4381 tagttgtaac aaccccattt gcatcgtttg tcttggttat cgtcactaga atcgtttctg
     4441 gatcagatgc caaggcaata ttagttaatc ccaacaatat tgtgaacagg taggtaaaat
     4501 gagcgaaaga taatgtcatt tatattgaat tttcaaaaat tcttactttt tttttggatg
     4561 gacgcaaaga agtttaataa tcatattaca tggcattacc accatataca tatccatatc
     4621 taatcttact tatatgttgt ggaaatgtaa agagccccat tatcttagcc taaaaaaacc
     4681 ttctctttgg aactttcagt aatacgctta actgctcatt gctatattga agtacggatt
     4741 agaagccgcc gagcgggtga cagccctccg aaggaagact ctcctccgtg cgtcctcgtc
     4801 ttcaccggtc gcgttcctga aacgcagatg tgcctcgcgc cgcactgctc cgaacaataa
     4861 agattctaca atactagctt ttatggttat gaagaggaaa aattggcagt aacctggccc
     4921 cacaaacctt caaatgaacg aatcaaatta acaaccatag gatgataatg cgattagttt
     4981 tttagcctta tttctggggt aattaatcag cgaagcgatg atttttgatc tattaacaga
     5041 tatataaatg caaaaactgc ataaccactt taactaatac tttcaacatt ttcggtttgt
     5101 attacttctt attcaaatgt aataaaagta tcaacaaaaa attgttaata tacctctata
     5161 ctttaacgtc aaggagaaaa aaccccggat cgaattccct acttcataca ttttcaatta
     5221 agatgcagtt acttcgctgt ttttcaatat tttctgttat tgcttcagtt ttagcacagg
     5281 aactgacaac tatatgcgag caaatcccct caccaacttt agaatcgacg ccgtactctt
     5341 tgtcaacgac tactattttg gccaacggga aggcaatgca aggagttttt gaatattaca
     5401 aatcagtaac gtttgtcagt aattgcggtt ctcacccctc aacaactagc aaaggcagcc
     5461 ccataaacac acagtatgtt tttaaggaca atagctcgac gattgaaggt agatacccat
     5521 acgacgttcc agactacgct ctgcaggcta gtggtggagg aggctctggt ggaggcggta
     5581 gcggaggcgg agggtcggct agcgatatcc agatgaccca gtccccgagc tccctgtccg
     5641 cctctgtgag cgatagggtc accatcacct gccgtgccgg tcagtacggt tcttcttacg
     5701 tagcctggta tcaacagaaa ccaggaaaag ctccgaagct tctgatttac tctgcatccc
     5761 acctctactc tggagtccct tctcgcttct ctggtagccg ttccgggacg ggtttcactc
     5821 tgaccatcag cagtctgcag ccggaagact tcgcaactta ttactgtcag caaccgggtc
     5881 cggctccggg tctgatcacg ttcggacagg gtaccaaggt ggagatcaaa ggtaccactg
     5941 ccgctagtgg tagtagtggt ggcagtagca gtggtgccga ggttcggctg gtggagtctg
     6001 gcggtggcct ggtgcagcca gggggctcac tccgtttgtc ctgtgcagct tctggcttca
     6061 acatctacta tggttcttac atccactggg tgcgtcaggc cccgggtaag ggcctggaat
     6121 gggttgcagg tatttctcct tactacaact ctacttacta tgccgatagc gtcaagggcc
     6181 gtttcactat aagcgcagac gcatccaaaa acacagccta cctacaaatg aacagcttaa
     6241 gagctgagga cactgccgtc tattattgtg ctcgcggtta cgcttctggt ggtatggact
     6301 actggggtca aggaaccctg gtcaccgtct cctcgggatc cgaacaaaag cttatttctg
     6361 aagaggactt gtaatagctc gagatctgat aacaacagtg tagatgtaac aaaatcgact
     6421 ttgttcccac tgtactttta gctcgtacaa aatacaatat acttttcatt tctccgtaaa
     6481 caacatgttt tcccatgtaa tatccttttc tatttttcgt tccgttacca actttacaca
     6541 tactttatat agctattcac ttctatacac taaaaaacta agacaatttt aattttgctg
     6601 cctgccatat ttcaatttgt tataaattcc tataatttat cctattagta gctaaaaaaa
     6661 gatgaatgtg aatcgaatcc taagagaatt gagctccaat tcggtcgacc tcgagggggg
     6721 gcccggtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     6781 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6841 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6901 tgcgcagcct gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6961 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     7021 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     7081 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     7141 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7201 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7261 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7321 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc
     7381 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa
     7441 ctgatataat taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa
     7501 tacagttttt tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct
     7561 gtaacgttca ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat
     7621 aataatgtca gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc
     7681 gtctcccttg tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc
     7741 tcttccaccc atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc
     7801 aatgtcaaca gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc
     7861 tgctaacatc aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc
     7921 gctaacaata cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat
     7981 tctgtataca cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct
     8041 gtcttcgaag agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc
     8101 ctccatggaa aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc
     8161 taatgcttca actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa
     8221 gtttgtttgc ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt
     8281 agcagcacgt tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt
     8341 tgttctgtgc agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat
     8401 gcgtatatat accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat
     8461 taccgaatca aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga
     8521 tgaattgaaa aggtggtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8581 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8641 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     8701 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     8761 aatgtcatga taataatggt ttcttagacg gatcgcttgc ctgtaactta cacgcgcctc
     8821 gtatctttta atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt
     8881 tgtatttgga ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa
     8941 aaataaacaa aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag
     9001 acaagaaaag cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca
     9061 caggattttc gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct
     9121 gagagcagga agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta
     9181 catcttcgga aaacaaaaac tat
//
