LOCUS       sequence-319624-        1242 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for His6-MEK1C218C222.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1242)
  AUTHORS   Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, 
            Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair 
            SK, Chen J, Davis BG, van der Donk WA
  TITLE     LanCLs add glutathione to dehydroamino acids generated at 
            phosphorylated sites in the proteome.
  JOURNAL   Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001.
  PUBMED    33932340
REFERENCE   2  (bases 1 to 1242)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001."
COMMENT     Sequence Label: MEK1C218C222
FEATURES             Location/Qualifiers
     source          1..1242
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgttcagca gccatcatca tcatcatcac agcagcggcc tggtgccgcg cggcagccat
       61 atgccgaaaa aaaaaccgac cccgattcaa ctgaacccgg ctccggatgg ctccgctgtc
      121 aatggcacct caagcgcaga aacgaacctg gaagcgctgc agaaaaaact ggaagaactg
      181 gaactggatg aacagcaacg taaacgcctg gaagcctttc tgacccagaa acaaaaagtg
      241 ggcgaactga aagatgacga tttcgaaaaa atcagtgaac tgggcgccgg taacggcggt
      301 gtggttttta aagtcagcca taaaccgtct ggtctggtga tggcacgtaa actgattcac
      361 ctggaaatca aaccggctat tcgtaaccag attatccgcg aactgcaagt gctgcatgaa
      421 tgcaattctc cgtatattgt tggcttttat ggtgcgttct acagtgacgg cgaaatttcc
      481 atctgtatgg aacacatgga cggcggtagc ctggatcagg ttctgaaaaa agcaggccgt
      541 atcccggaac aaattctggg taaagtctct attgctgtga tcaaaggcct gacgtacctg
      601 cgtgaaaaac ataaaatcat gcaccgcgat gtgaaaccgt caaacatcct ggttaattcg
      661 cgcggtgaaa ttaaactgtg cgactttggc gttagcggtc agctgattga ttgtatggcg
      721 aactgcttcg tcggcacccg tagttatatg tccccggaac gcctgcaggg tacgcattac
      781 tcagtgcaat cggatatctg gtcaatgggc ctgtcgctgg ttgaaatggc cgttggtcgt
      841 tatccgattc cgccgccgga cgccaaagaa ctggaactga tgtttggctc tcaggttgaa
      901 ggtgatgcgg ccgaaacccc gccgcgtccg cgtaccccgg gtcgtccgct gagctcttat
      961 ggtatggaca gccgtccgcc gatggcaatc ttcgaactgc tggattacat tgtgaatgaa
     1021 ccgccgccaa aactgccgag cggcgtgttt agcctggaat ttcaggactt cgtcaacaaa
     1081 tgtctgatca aaaatccggc agaacgtgct gatctgaaac aactgatggt ccacgctttt
     1141 attaaacgct ccgacgcgga agaagtggat ttcgccggtt ggctgtcttc gaccattggc
     1201 ctgaatcaac cgtcaacgcc gacccacgct gctggtgtgt aa
//
