LOCUS       sequence-319626-       12054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  overexpression in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12054)
  AUTHORS   Yang LZ, Wang Y, Li SQ, Yao RW, Luan PF, Wu H, Carmichael GG, Chen 
            LL
  TITLE     Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems.
  JOURNAL   Mol Cell. 2019 Dec 19;76(6):981-997.e7. doi: 
            10.1016/j.molcel.2019.10.024. Epub 2019 Nov 19.
  PUBMED    31757757
REFERENCE   2  (bases 1 to 12054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2019.10.024"; journalName: "Mol Cell"; date: 
            "2019-12-19- 19"; volume: "76"; issue: "6"; pages: "981-997.e7"
COMMENT     Sequence Label: pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag
FEATURES             Location/Qualifiers
     source          1..12054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2223..3401
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2454..3392
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3349..3369
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             3414..3461
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      6351..6360
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6357..6359,6360..6362,6363..7073)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6357..6359,6360..6362,6363..7073)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6663..6682)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7010..7031
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7101..7148
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7167..7232
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    7239..7806
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7400..7417)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7627..7646
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7826..8425
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7826..7845)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8322..8342
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8444..9032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8497..8517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8915..8926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9107..9340
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9459..9477)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9545..9649
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9650..9718,9719..10510)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9868..9887)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10681..11269
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11170..11189
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11423..11440
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11680..11701
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11716..11721,11722..11739,11740..11746)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11754..11770
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11759..11781
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11778..11794
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11778..11794
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11844..11863
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt
     2581 gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct
     2641 ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg
     2701 ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct
     2761 ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg
     2821 ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc
     2881 gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc
     2941 ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac
     3001 cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga
     3061 ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc
     3121 cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg
     3181 attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga
     3241 tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt
     3301 aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga
     3361 cagtggttca aagttttttt cttccatttc aggtgtcgtg aagcggccgc atgaagcgac
     3421 ctgccgccac aaagaaggct ggacaggcta agaagaagaa agctagcatg ggcaagaaga
     3481 tccacgcccg ggacctgaga gagcagagga agaccgaccg gacagagaag ttcgccgatc
     3541 agaacaagaa gagggaggca gagagggcag tgcccaagaa ggacgccgcc gtgagcgtga
     3601 agtccgtgag ctccgtgtct agcaagaagg ataatgtgac caagtctatg gccaaggcag
     3661 caggagtgaa gagcgtgttc gcagtgggca acaccgtgta catgacatcc tttggcagag
     3721 gcaatgacgc cgtgctggag cagaagatcg tggatacatc tcacgagcca ctgaacatcg
     3781 acgatcccgc ctaccagctg aatgtggtga ccatgaacgg ctattccgtg acaggacaca
     3841 ggggagagac cgtgtctgcc gtgacagaca acccactgcg gagattcaat ggccgcaaga
     3901 aggacgagcc cgagcagtct gtgcctaccg atatgctgtg cctgaagccc acactggaga
     3961 agaagttctt tggcaaggag tttgacgata acatccacat ccagctgatc tacaatatcc
     4021 tggacatcga gaagatcctg gccgtgtaca gcaccaacgc catctatgcc ctgaacaata
     4081 tgagcgccga cgagaatatc gagaactccg atttctttat gaagaggacc acagatgaga
     4141 ccttcgacga ttttgagaag aagaaggagt ctacaaacag ccgggagaag gccgacttcg
     4201 atgcctttga gaagttcatc ggcaattaca gactggccta ttttgccgac gccttctacg
     4261 tgaataagaa gaaccccaag ggcaaggcca agaatgtgct gcgcgaggat aaggagctgt
     4321 attctgtgct gaccctgatc ggcaagctgg cccactggtg cgtggccagc gaggagggaa
     4381 gagcagagtt ctggctgtac aagctggacg agctgaagga cgacttcaag aacgtgctgg
     4441 atgtggtgta taataggcct gtggaggaga tcaacaatcg cttcatcgag aacaataagg
     4501 tgaacatcca gatcctgggc agcgtgtaca agaataccga catcgccgag ctggtgagat
     4561 cctactatga gttcctgatc acaaagaagt ataagaacat gggcttttcc atcaagaagc
     4621 tgcgggagtc tatgctggag ggcaagggct acgccgacaa ggagtatgat tctgtgagaa
     4681 ataagctgta ccagatgacc gacttcatcc tgtacacagg ctatatcaac gaggacagcg
     4741 atagggccga cgatctggtg aataccctgc gctcctctct gaaggaggac gataagacca
     4801 cagtgtattg caaggaggcc gactacctgt ggaagaagta tcgggagtcc atcagagagg
     4861 tggccgatgc cctggacggc gataacatca agaagctgag caagtccaat atcgagatcc
     4921 aggaggacaa gctgaggaag tgttttatca gctacgccga ttctgtgagc gagttcacca
     4981 agctgatcta tctgctgaca cgctttctgt ccggcaagga gatcaacgac ctggtgacca
     5041 cactgatcaa taagtttgat aacatcaggt ctttcctgga gatcatggac gagctgggcc
     5101 tggatcgcac cttcacagcc gagtacagct tctttgaggg ctccaccaag tatctggccg
     5161 agctggtgga gctgaacagc tttgtgaagt cctgctcttt cgacatcaat gccaagagga
     5221 ccatgtaccg cgacgccctg gatatcctgg gcatcgagtc cgacaagaca gaggaggata
     5281 tcgagaagat gatcgacaac atcctgcaga tcgacgccaa tggcgataag aagctgaaga
     5341 agaacaatgg cctgcggaat ttcatcgcct ctaacgtgat cgatagcaat cggtttaagt
     5401 acctggtgag atatggcaac cctaagaaga tcagggagac cgccaagtgc aagcctgccg
     5461 tgcgcttcgt gctgaatgag atcccagatg cccagatcga gcggtactat gaggcctgct
     5521 gtccaaagaa caccgccctg tgcagcgcca ataagaggcg cgagaagctg gccgacatga
     5581 tcgccgagat caagtttgag aacttctccg atgccggcaa ctaccagaag gccaatgtga
     5641 cctccagaac atctgaggcc gagatcaaga ggaagaacca ggccatcatc cgcctgtacc
     5701 tgaccgtgat gtatatcatg ctgaagaatc tggtgaacgt gaatgccaga tacgtgatcg
     5761 cctttcactg cgtggagaga gacacaaagc tgtatgccga gagcggcctg gaggtcggca
     5821 acatcgagaa gaataagacc aacctgacaa tggccgtgat gggcgtgaag ctggagaatg
     5881 gcatcatcaa gaccgagttt gacaagtcct tcgccgagaa tgccgccaac cgctacctgc
     5941 ggaacgccag atggtataag ctgatcctgg ataatctgaa gaagtctgag cgggccgtgg
     6001 tgaatgagtt cgccaacacc gtgtgcgccc tgaacgccat caggaacatc aatatcaaca
     6061 tcaaggagat caaggaggtg gagaactact ttgccctgta ccactatctg atccagaagc
     6121 acctggagaa tcggttcgcc gacaagaagg tggagagaga caccggcgac ttcatctcca
     6181 agctggagga gcacaagacc tactgcaagg actttgtgaa ggcctattgt acacccttcg
     6241 gctacaacct ggtgcgctat aagaatctga caatcgacgg cctgttcgat aagaactacc
     6301 ctggcaagga cgatagcgac gagcagaagg gtctagaggc gctaccggtc gccaccatgg
     6361 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     6421 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     6481 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     6541 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     6601 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     6661 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     6721 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     6781 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     6841 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     6901 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     6961 tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     7021 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtcgaatt
     7081 ctaagccagg attccatatg aagcgacctg ccgccacaaa gaaggctgga caggctaaga
     7141 agaagaaaca acgcgtgcag ctaagcgact acaaagacca tgacggtgat tataaagatc
     7201 atgacatcga ttacaaggat gacgatgaca agtaaggatc cctccccccc ccctaacgtt
     7261 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     7321 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     7381 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     7441 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     7501 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     7561 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     7621 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     7681 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     7741 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     7801 tgataaggat ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
     7861 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     7921 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     7981 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     8041 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     8101 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     8161 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     8221 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     8281 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     8341 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     8401 catgacccgc aagcccggtg cctgacgccc atcgatagat cctaatcaac ctctggatta
     8461 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     8521 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     8581 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     8641 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     8701 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     8761 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     8821 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     8881 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     8941 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     9001 gagtcggatc tccctttggg ccgcctcccc gcctgagatc ctttaagacc aatgacttac
     9061 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     9121 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     9181 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9241 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9301 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     9361 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gcccgggtta
     9421 attaaggaaa gggctagatc attcttgaag acgaaagggc ctcgtgatac gcctattttt
     9481 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     9541 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9601 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9661 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9721 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     9781 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     9841 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     9901 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     9961 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    10021 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10081 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10141 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10201 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10261 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10321 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    10381 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10441 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10501 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10561 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10621 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10681 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10741 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10801 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    10861 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10921 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10981 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    11041 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    11101 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    11161 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    11221 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    11281 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    11341 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    11401 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
    11461 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcaagc tcatggctga
    11521 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
    11581 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctccccgt ggcacgacag
    11641 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    11701 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    11761 cggataacaa tttcacacag gaaacagcta tgacatgatt acgaatttca caaataaagc
    11821 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
    11881 ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca ccccataccc
    11941 tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt cctctgaatt
    12001 attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag tagt
//
