LOCUS       sequence-319634-        3521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression plasmid for His6-ERK1C82C202.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Lai KY, Galan SRG, Zeng Y, Zhou TH, He C, Raj R, Riedl J, Liu S, 
            Chooi KP, Garg N, Zeng M, Jones LH, Hutchings GJ, Mohammed S, Nair 
            SK, Chen J, Davis BG, van der Donk WA
  TITLE     LanCLs add glutathione to dehydroamino acids generated at 
            phosphorylated sites in the proteome.
  JOURNAL   Cell. 2021 Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001.
  PUBMED    33932340
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2021 
            Apr 27. pii: S0092-8674(21)00436-0. doi: 
            10.1016/j.cell.2021.04.001."
COMMENT     Sequence Label: ERK1C82C202
FEATURES             Location/Qualifiers
     source          1..3521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(1275..2066,2067..2135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1898..1917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2136..2240)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2308..2326
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2455..3043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2944..2963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3197..3214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     3423..3445
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     3496..3515
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3496..3514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattcatgg cacatcacca tcaccatcat atggcggcgg cggcggctca ggggggcggg
       61 ggcggggagc cccgtagaac cgagggggtc ggcccggggg tcccggggga ggtggagatg
      121 gtgaaggggc agccgttcga cgtgggcccg cgctacacgc agttgcagta catcggcgag
      181 ggcgcgtacg gcatggtcag ctcggcctat gaccacgtgc gcaagactcg cgtggccatc
      241 cgtaagatca gccccttcga acatcagacc tactgccagc gcacgctccg ggagatccag
      301 atcctgctgc gcttccgcca tgagaatgtc atcggcatcc gagacattct gcgggcgtcc
      361 accctggaag ccatgagaga tgtctacatt gtgcaggacc tgatggagac tgacctgtac
      421 aagttgctga aaagccagca gctgagcaat gaccatatca gctacttcct ctaccagatc
      481 ctgcggggcc tcaagtacat ccactccgcc aacgtgctcc accgagatct aaagccctcc
      541 aacctgctca tcaacaccac cagcgacctt aagatttgtg atttcggcct ggcccggatt
      601 gccgatcctg agcatgacca caccggcttc ctgtgcgagt atgtggctac gcgctggtac
      661 cgggccccag agatcatgct gaactccaag ggctatacca agtccatcga catctggtct
      721 gtgggctgca ttctggctga gatgctctct aaccggccca tcttccctgg caagcactac
      781 ctggatcagc tcaaccacat tctgggcatc ctgggctccc catcccagga ggacctgaat
      841 agtatcatca acatgaaggc ccgaaactac ctacagtctc tgccctccaa gaccaaggtg
      901 gcttgggcca agcttttccc caagtcagac tccaaagccc ttgacctgct ggaccggatg
      961 ttaaccttta accccaataa acggatcaca gtggaggaag cgctggctca cccctacctg
     1021 gagcagtact atgacccgac ggatgagcca gtggccgagg agcccttcac cttcgccatg
     1081 gagctggatg acctacctaa ggagcggctg aaggagctca tcttccagga gacagcacgc
     1141 ttccagcccg gagtgctgga ggccccctag ggatcctcta gagtcgacct gcagcccaag
     1201 cttatcgatg ataagctgtc aaacatgaga attaaatcaa tctaaagtat atatgagtaa
     1261 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     1321 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     1381 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     1441 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     1501 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     1561 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     1621 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     1681 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     1741 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     1801 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     1861 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     1921 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     1981 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     2041 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     2101 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     2161 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     2221 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     2281 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     2341 gaataaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     2401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     2461 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     2521 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     2581 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     2641 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     2701 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     2761 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     2821 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     2881 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     2941 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3001 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3061 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     3121 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     3181 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta
     3241 ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat
     3301 ctgctctgat gcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     3361 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     3421 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcccag
     3481 ctggcttatc gaaattaata cgactcacta tagggagacc g
//
