LOCUS       sequence-319640-        9225 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CHRNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9225)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CHRNB1_OE_1
FEATURES             Location/Qualifiers
     source          1..9225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2059..2083)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2085..2126
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2168..2756
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2221..2241)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2639..2650)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2828..3061
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3139..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3176..3195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3230..3249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3300..3435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3455..3474)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3456..3474)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3484..3501)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3484..3500)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3493..3515)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3642..4097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3729..3748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3939..3960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4123..4227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4228..4296,4297..5088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4446..4465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5259..5847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5748..5767
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6001..6018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6135..6156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6171..6176,6177..6194,6195..6201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6209..6225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6214..6236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6233..6249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6233..6249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6268..6288
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6270..6288
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6316..6542
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6543..6723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6770..6895
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7388..7621
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7806..7850
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8025..8525
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8444..8462
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8537..8932
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8992..9109
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatgacc ccaggggctc tgctgatgct gctgggggcg ctgggggcgc
      601 cgctcgcccc aggcgtccgc ggctcggagg cggagggtcg actccgggag aaacttttct
      661 ctggctatga tagctccgtg cggccagcgc gggaggtggg agaccgtgtc agggtcagcg
      721 ttggtctcat cctggcgcaa ctcatcagcc tgaacgagaa ggatgaagag atgagcacaa
      781 aggtgtactt agacctggag tggactgact acaggctgag ctgggaccct gcggagcacg
      841 acggcatcga ttcgctccgc atcacggcgg aatccgtgtg gctccctgac gtggtgctac
      901 tgaacaacaa tgatgggaat tttgacgtgg ctctggacat tagcgtcgtg gtgtcctccg
      961 acggctccgt gcgttggcaa cccccgggca tctatcgcag cagctgcagc atccaggtca
     1021 cctacttccc cttcgactgg cagaattgca ctatggtgtt cagctcctac agctacgaca
     1081 gctcggaggt cagcctgcag acaggcctgg gtcctgacgg gcaagggcat caggaaatcc
     1141 acattcatga agggactttc attgagaatg gccagtggga gattatccac aagccctctc
     1201 ggctaatcca gcctccaggc gatcctaggg gagggaggga aggacagcgc caggaagtca
     1261 tcttctacct catcatccgc cgcaagcctc tcttctacct ggtcaacgtc attgccccat
     1321 gcatcctcat cactcttctg gccatcttcg tcttctacct gccaccagat gcaggagaga
     1381 agatggggct ctcaatcttt gccctgctga cccttactgt gttcctgctg ctgctggctg
     1441 acaaagtacc tgagacctca ctatcagtac ccattattat caagtacctc atgtttacca
     1501 tggtcctcgt caccttctca gtcatcctta gtgtcgtggt tctcaacctg caccaccgct
     1561 caccccacac ccaccaaatg cccctttggg tccgtcagat cttcattcac aaacttccgc
     1621 tgtacctgcg tctaaaaagg cccaaacccg agagagacct gatgccggag ccccctcact
     1681 gttcttctcc aggaagtggc tggggtcggg gaacagatga atatttcatc cggaagccgc
     1741 caagtgattt tctcttcccc aaacccaata ggttccagcc tgaactgtct gcccctgatc
     1801 tgcggcgatt tatcgatggt ccaaaccggg ctgtggccct gcttccggag ctacgggagg
     1861 tcgtctcctc tatcagctac atcgctcgac agctgcagga acaggaggac cacgatgcgc
     1921 tgaaggagga ctggcagttt gtggccatgg tagtggaccg cctcttcctg tggactttca
     1981 tcatcttcac cagcgttggg accctagtca tcttcctgga cgccacgtac cacttgcccc
     2041 ctccagaccc ctttccttgc ccaactttct tgtacaaagt ggttggtaag cctatcccta
     2101 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
     2161 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2221 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2281 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2341 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     2401 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2461 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2521 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2581 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2641 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2701 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     2761 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     2821 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     2881 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2941 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     3001 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     3061 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     3121 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     3181 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     3241 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     3301 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     3361 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3421 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     3481 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3541 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3601 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3661 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3721 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3781 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3841 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3901 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3961 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4021 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4081 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4141 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4201 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4261 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4321 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4381 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4441 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4501 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4561 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4621 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4681 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4741 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4801 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4861 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4921 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4981 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5041 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5101 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5161 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5221 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5281 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5341 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5401 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5461 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5521 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5581 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5641 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5701 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5761 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5821 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5881 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5941 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     6001 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     6061 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     6121 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     6181 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     6241 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     6301 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     6361 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     6421 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     6481 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     6541 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6601 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6661 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6721 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6781 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6841 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6901 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6961 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     7021 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     7081 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     7141 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     7201 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     7261 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     7321 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     7381 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7441 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7501 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7561 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7621 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7681 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7741 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7801 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7861 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7921 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7981 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     8041 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     8101 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     8161 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     8221 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     8281 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     8341 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     8401 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     8461 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     8521 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     8581 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     8641 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     8701 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     8761 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     8821 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     8881 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     8941 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     9001 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9061 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     9121 cagggacagc agagatccac tttggcgccg gctcgagggg gcccggggtt attaatagta
     9181 atcaattacg gggtcattag ttcatagccc atatatggag ttccg
//
