LOCUS       sequence-319641-        9498 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CACNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9498)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9498)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNB1_OE_1
FEATURES             Location/Qualifiers
     source          1..9498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2332..2356)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2358..2399
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2441..3029
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2494..2514)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2912..2923)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3101..3334
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3412..3533
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3449..3468)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3503..3522
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3573..3708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3728..3747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3729..3747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3757..3774)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3757..3773)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3766..3788)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3915..4370
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4002..4021)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4212..4233
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4396..4500
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4501..4569,4570..5361)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4719..4738)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5532..6120
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6021..6040
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6274..6291
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6408..6429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6444..6449,6450..6467,6468..6474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6482..6498
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6487..6509
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6506..6522
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6506..6522
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6541..6561
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6543..6561
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6589..6815
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6816..6996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7043..7168
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7661..7894
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8079..8123
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8298..8798
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8717..8735
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8810..9205
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9265..9382
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatgtcc cggggccctt acccaccctc ccaggagatc cccatggagg
      601 tcttcgaccc cagcccgcag ggcaaataca gcaagaggaa agggcgattc aaacggtcag
      661 atgggagcac gtcctcggat accacatcca acagctttgt ccgccagggc tcagcggagt
      721 cctacaccag ccgtccatca gactctgatg tatctctgga ggaggaccgg gaagccttaa
      781 ggaaggaagc agagcgccag gcattagcgc agctcgagaa ggccaagacc aagccagtgg
      841 catttgctgt gcggacaaat gttggctaca atccgtctcc aggggatgag gtgcctgtgc
      901 agggagtggc catcaccttc gagcccaaag acttcctgca catcaaggag aaatacaata
      961 atgactggtg gatcgggcgg ctggtgaagg agggctgtga ggttggcttc attcccagcc
     1021 ccgtcaaact ggacagcctt cgcctgctgc aggaacagaa gctgcgccag aaccgcctcg
     1081 gctccagcaa atcaggcgat aactccagtt ccagtctggg agatgtggtg actggcaccc
     1141 gccgccccac accccctgcc agtgccaaac agaagcagaa gtcgacagag catgtgcccc
     1201 cctatgacgt ggtgccttcc atgaggccca tcatcctggt gggaccgtcg ctcaagggct
     1261 acgaggttac agacatgatg cagaaagctt tatttgactt cttgaagcat cggtttgatg
     1321 gcaggacctc catcactcgt gtgacggcag atatttccct ggctaagcgc tcagttctca
     1381 acaaccccag caaacacatc atcattgagc gctccaacac acgctccagc ctggctgagg
     1441 tgcagagtga aatcgagcga atcttcgagc tggcccggac ccttcagttg gtcgctctgg
     1501 atgctgacac catcaatcac ccagcccagc tgtccaagac ctcgctggcc cccatcattg
     1561 tttacatcaa gatcacctct cccaaggtac ttcaaaggct catcaagtcc cgaggaaagt
     1621 ctcagtccaa acacctcaat gtccaaatag cggcctcgga aaagctggca cagtgccacc
     1681 ctgaaatgtt tgacatcatc ctggatgaga accaattgga ggatgcctgc gagcatctgg
     1741 cggagtactt ggaagcctat tggaaggcca cacacccgcc cagcagcacg ccacccaatc
     1801 cgctgctgaa ccgcaccatg gctaccgcag ccctggctgc cagccctgcc cctgtctcca
     1861 acctccaggg accctacctt gcttccgggg accagccact ggaacgggcc accggggagc
     1921 acgccagcat gcacgagtac ccaggggagc tgggccagcc cccaggcctt taccccagca
     1981 gccacccacc aggccgggca ggcacgctac gggcactgtc ccgccaagac acttttgatg
     2041 ccgacacccc cggcagccga aactctgcct acacggagct gggagactca tgtgtggaca
     2101 tggagactga cccctcagag gggccagggc ttggagaccc tgcagggggc ggcacgcccc
     2161 cagcccgaca gggatcctgg gaggacgagg aagaagacta tgaggaagag ctgaccgaca
     2221 accggaaccg gggccggaat aaggcccgct actgcgctga gggtgggggt ccagttttgg
     2281 ggcgcaacaa gaatgagctg gagggctggg gacgaggcgt ctacattcgc tgcccaactt
     2341 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
     2401 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggattacaa
     2461 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     2521 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     2581 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     2641 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     2701 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     2761 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     2821 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     2881 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     2941 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     3001 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     3061 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     3121 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     3181 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     3241 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     3301 agaccctttt agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt
     3361 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
     3421 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     3481 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     3541 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
     3601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
     3661 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc
     3721 caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg
     3781 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
     3841 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
     3901 gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3961 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4021 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4081 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4141 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4201 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4261 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4321 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact
     4381 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4441 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4501 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4561 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4621 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4681 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4741 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4801 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4861 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4921 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4981 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5041 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5101 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5161 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5221 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5281 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5341 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5401 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5461 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5521 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5581 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5641 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5701 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5761 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5821 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5881 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5941 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6001 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6061 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6121 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6181 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6241 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6301 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     6361 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
     6421 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     6481 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc
     6541 gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg
     6601 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
     6661 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
     6721 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag
     6781 atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct
     6841 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     6901 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     6961 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7021 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7081 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7141 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7201 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7261 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7321 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7381 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7441 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7501 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7561 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7621 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7681 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     7741 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7801 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7861 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7921 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7981 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8041 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8101 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8161 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8221 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8281 caaccccgag gggacccttg cgccttttcc aaggcagccc tgggtttgcg cagggacgcg
     8341 gctgctctgg gcgtggttcc gggaaacgca gcggcgccga ccctgggtct cgcacattct
     8401 tcacgtccgt tcgcagcgtc acccggatct tcgccgctac ccttgtgggc cccccggcga
     8461 cgcttcctgc tccgccccta agtcgggaag gttccttgcg gttcgcggcg tgccggacgt
     8521 gacaaacgga agccgcacgt ctcactagta ccctcgcaga cggacagcgc cagggagcaa
     8581 tggcagcgcg ccgaccgcga tgggctgtgg ccaatagcgg ctgctcagca gggcgcgccg
     8641 agagcagcgg ccgggaaggg gcggtgcggg aggcggggtg tggggcggta gtgtgggccc
     8701 tgttcctgcc cgcgcggtgt tccgcattct gcaagcctcc ggagcgcacg tcggcagtcg
     8761 gctccctcgt tgaccgaatc accgacctct ctccccaggg ggtaccacca tggccaagcc
     8821 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     8881 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     8941 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     9001 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     9061 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     9121 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     9181 gccctctggt tatgtgtggg agggcctgca gctgcagtag taagaattct agatcttgag
     9241 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     9301 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     9361 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     9421 ccggctcgag ggggcccggg gttattaata gtaatcaatt acggggtcat tagttcatag
     9481 cccatatatg gagttccg
//
