LOCUS       sequence-319645-        9203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Yeast display reporter for non-display yeast strains (TAG 
            construct).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Potts KA, Stieglitz JT, Lei M, Van Deventer JA
  TITLE     Reporter system architecture affects measurements of noncanonical 
            amino acid incorporation efficiency and fidelity.
  JOURNAL   Mol Syst Des Eng. 2020 Feb 1;5(2):573-588. doi: 10.1039/c9me00107g. 
            Epub 2020 Jan 23.
  PUBMED    33791108
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1039/c9me00107g"; journalName: "Mol Syst Des Eng"; date: 
            "2020-02-1- 1"; volume: "5"; issue: "2"; pages: "573-588"
COMMENT     Sequence Label: pRS416-Aga1p-FAPB2.3.6L1TAG
FEATURES             Location/Qualifiers
     source          1..9203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..118
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     413..436
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             768..779
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             780..806
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1608..1637
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2004..2023)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2005..2023)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2033..2050)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2033..2049)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2042..2064)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2190..2645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2277..2296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2487..2508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2776..3579)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(3580..3795)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(3819..3838)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3938..3960
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3998..4016)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    4057..4560
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        4597..4701
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4702..4770,4771..5562)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4920..4939)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5733..6321
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6222..6241
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6475..6492
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6609..6630
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6645..6650,6651..6668,6669..6675)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6683..6699
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6688..6710
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6707..6723
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6707..6723
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6742..6762
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6744..6762
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(9000..9203,1..449)
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
ORIGIN
        1 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
       61 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      121 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      181 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
      241 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      301 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
      361 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
      421 tctatacttt aacgtcaagg agaaaaaacc ccggatcgaa ttccctactt catacatttt
      481 caattaagat gcagttactt cgctgttttt caatattttc tgttattgct tcagttttag
      541 cacaggaact gacaactata tgcgagcaaa tcccctcacc aactttagaa tcgacgccgt
      601 actctttgtc aacgactact attttggcca acgggaaggc aatgcaagga gtttttgaat
      661 attacaaatc agtaacgttt gtcagtaatt gcggttctca cccctcaaca actagcaaag
      721 gcagccccat aaacacacag tatgttttta aggacaatag ctcgacgatt gaaggtagat
      781 acccatacga cgttccagac tacgctctgc aggctagtgg tggaggaggc tctggtggag
      841 gcggtagcgg aggcggaggg tcggctagct agatccagat gacccagtcc ccgagctccc
      901 tgtccgcctc tgtgagcgat agggtcacca tcacctgccg tgccggtcag tacggttctt
      961 cttacgtagc ctggtatcaa cagaaaccag gaaaagctcc gaagcttctg atttactctg
     1021 catcccacct ctactctgga gtcccttctc gcttctctgg tagccgttcc gggacgggtt
     1081 tcactctgac catcagcagt ctgcagccgg aagacttcgc aacttattac tgtcagcaac
     1141 cgggtccggc tccgggtctg atcacgttcg gacagggtac caaggtggag atcaaaggta
     1201 ccactgccgc tagtggtagt agtggtggca gtagcagtgg tgccgaggtt cggctggtgg
     1261 agtctggcgg tggcctggtg cagccagggg gctcactccg tttgtcctgt gcagcttctg
     1321 gcttcaacat ctactatggt tcttacatcc actgggtgcg tcaggccccg ggtaagggcc
     1381 tggaatgggt tgcaggtatt tctccttact acaactctac ttactatgcc gatagcgtca
     1441 agggccgttt cactataagc gcagacgcat ccaaaaacac agcctaccta caaatgaaca
     1501 gcttaagagc tgaggacact gccgtctatt attgtgctcg cggttacgct tctggtggta
     1561 tggactactg gggtcaagga accctggtca ccgtctcctc gggatccgaa caaaagctta
     1621 tttctgaaga ggacttgtaa tagctcgaga tctgataaca acagtgtaga tgtaacaaaa
     1681 tcgactttgt tcccactgta cttttagctc gtacaaaata caatatactt ttcatttctc
     1741 cgtaaacaac atgttttccc atgtaatatc cttttctatt tttcgttccg ttaccaactt
     1801 tacacatact ttatatagct attcacttct atacactaaa aaactaagac aattttaatt
     1861 ttgctgcctg ccatatttca atttgttata aattcctata atttatccta ttagtagcta
     1921 aaaaaagatg aatgtgaatc gaatcctaag agaattgagc tccaattcgg tcgacctcga
     1981 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     2041 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     2101 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     2161 aacagttgcg cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc
     2221 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     2281 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     2341 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2401 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     2461 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2521 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2581 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2641 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataggg
     2701 taataactga tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac
     2761 ttataataca gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc
     2821 ttttctgtaa cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc
     2881 aacaataata atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc
     2941 caatgcgtct cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc
     3001 ttcatctctt ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct
     3061 cttcgcaatg tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga
     3121 caattctgct aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt
     3181 caaaccgcta acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc
     3241 tgctattctg tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa
     3301 ttttctgtct tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac
     3361 tgtgccctcc atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt
     3421 gggacctaat gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc
     3481 acacaagttt gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg
     3541 atgagtagca gcacgttcct tatatgtagc tttcgacatg atttatcttc gtttcctgca
     3601 ggtttttgtt ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt cttcaacact
     3661 acatatgcgt atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgttc
     3721 ggagattacc gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa
     3781 aaatgatgaa ttgaaaaggt ggtatggtgc actctcagta caatctgctc tgatgccgca
     3841 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3901 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3961 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
     4021 taggttaatg tcatgataat aatggtttct tagacggatc gcttgcctgt aacttacacg
     4081 cgcctcgtat cttttaatga tggaataatt tgggaattta ctctgtgttt atttattttt
     4141 atgttttgta tttggatttt agaaagtaaa taaagaaggt agaagagtta cggaatgaag
     4201 aaaaaaaaat aaacaaaggt ttaaaaaatt tcaacaaaaa gcgtacttta catatatatt
     4261 tattagacaa gaaaagcaga ttaaatagat atacattcga ttaacgataa gtaaaatgta
     4321 aaatcacagg attttcgtgt gtggtcttct acacagacaa gatgaaacaa ttcggcatta
     4381 atacctgaga gcaggaagag caagataaaa ggtagtattt gttggcgatc cccctagagt
     4441 cttttacatc ttcggaaaac aaaaactatt ttttctttaa tttctttttt tactttctat
     4501 ttttaattta tatatttata ttaaaaaatt taaattataa ttatttttat agcacgtgat
     4561 gaaaaggacc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4621 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4681 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4741 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4801 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4861 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4921 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4981 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5041 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5101 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5161 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5221 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5281 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5341 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5401 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5461 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5521 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5581 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5641 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5701 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5761 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5821 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5881 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5941 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6001 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6061 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6121 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6181 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6241 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6301 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6361 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6421 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6481 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6541 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6601 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6661 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6721 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctc
     6781 caccgcggtg gcggccgctc tagaggtacc ttaactgaaa attacattgc aagcaactgc
     6841 catgatggcg aagaccaact ttcctaatgg caaacgcgtt tgcgatccac tgcccatata
     6901 ttggctaatt gttaccattg atgaagtttg gtattgtatg gagattgaag tagtggaaac
     6961 ttctgtagtg gtaacagatg tttgtagaga agagggagca gatgatgatg agaggaaaat
     7021 agaagatacc gtcggagtca atgatgaaga ttcaatagtc gattccacag aggtagatgg
     7081 tgtagagata gatgttgcgg aaagtgatga tgtagaggca gatattgtag caatagctgt
     7141 ggaagaagtg ggttgtaaaa tctctgatgt tgaagaaatt tgaagtgtgg taactggtga
     7201 ctgttcgaca cttagtgatg atacagaact gaatagcaaa atagtactgt cagaggataa
     7261 tgtagcagac aaggaggagg atgaaaggac cgatgtcgat aataagtgtg atgattgttc
     7321 ttgagattct gagtcaatgc ttgatgtact cgtggcttca cttacactag tcgaaatggt
     7381 tgaggtacct gaagatgtta catgatactt accggtagcg tgcttattga aatcttcatc
     7441 cgaagttgtc attgaactta cccaactctt tgtggatgtg gtctgctgtg ttacagaact
     7501 tgatgaagct gtttgcacgg aagaatacga gtgtgacgtt tcagcacatg tggagcatgc
     7561 atatgaggat tcaaccgttg aggtgcttac agaagttgcg acgtttttag tggacatttt
     7621 tatagaagat gtaatcaaac ttgttgtctg cgagggcatg caagactcgt gagtgcagac
     7681 tgtagcgtct gttgtaaccg tttcatgcat actggttaat gtgctagtct cagattcaga
     7741 gctataagga caccatgtcg tatacattgt agtgactcca ctaacagtgg tgaaatatgt
     7801 agacatggaa aatgatggga tagtagtaga aattgaggat ttggtgatat attcagacga
     7861 actttgtgaa ctaacagttg tttcaacagt tgaagaagtc atagaaggag ttgcaacatt
     7921 tgacgaagtc gaagggacgg agtaaacagg tgtggatggg ctgtataatg acacagacgt
     7981 tgatgaagat gctatagagg agccaaggga tgaagttgaa tcagtaaaag tagagctaat
     8041 agatgttgaa cttggagaag tggaagccaa agttggagat gaagaagtca aacttgggga
     8101 tgtagatgtt gaatatgaag aagtggaagt ggagcttgca gaagtagatt tagaacttgg
     8161 agatgtagat gttgaacttg atgaggtaga tgtagagctt ggagatgtag atgtcgaact
     8221 tgatgaggta gatgtagaac tttgggatgt agatgtagaa cttgaagatg tggatgtcaa
     8281 acttgaagat gtggatgtcg aacttggaga tgtagatgtc gaacttgatg aggtagatgt
     8341 cgaacttgat gaggtagatg tcgaacttga tgaggtagat gtagagcttg gagatgtaga
     8401 tgtagagctt ggagatgtag atgtcgaact tagggaggta gtagttggat tggacgatgt
     8461 tgtactcgaa agtgtagagg tgacaggtga gattatagag gcacttgatg gttcaatggc
     8521 gctggatgat accacggttg tagttcctac ttcggataat gagctgatag cggttgtgtg
     8581 acaagtaggg caaatatatg aagtgaactt ggaagtcaca gataaggtgg tggttggcaa
     8641 cgtcgatgac gaaggacatg cctcatggga gcagacttct gtagataatg tcaaagtact
     8701 aaatctggat agggtagtag cgtacgatat tgaaggactt atttcggcag cagatgaagt
     8761 ggataccgtc aatggacacc aagtcgtata caatgtcgta gtgccagctt gaacgatagt
     8821 ggatgtggag actagcgcgg gtgaaactgt agttgtaaca accccatttg catcgtttgt
     8881 cttggttatc gtcactagaa tcgtttctgg atcagatgcc aaggcaatat tagttaatcc
     8941 caacaatatt gtgaacaggt aggtaaaatg agcgaaagat aatgtcattt atattgaatt
     9001 ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat
     9061 ggcattacca ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa
     9121 gagccccatt atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa
     9181 ctgctcattg ctatattgaa gta
//
