LOCUS       sequence-319648-       10332 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CLCN6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10332)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 10332)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CLCN6_OE_1
FEATURES             Location/Qualifiers
     source          1..10332
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3166..3190)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3192..3233
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3275..3863
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3328..3348)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3746..3757)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3935..4168
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4246..4367
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4283..4302)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4337..4356
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4407..4542
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4562..4581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4563..4581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4591..4608)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4591..4607)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4600..4622)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4749..5204
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4836..4855)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5046..5067
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5230..5334
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5335..5403,5404..6195)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5553..5572)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6366..6954
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6855..6874
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7108..7125
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7242..7263
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7278..7283,7284..7301,7302..7308)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7316..7332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7321..7343
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7340..7356
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7340..7356
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7375..7395
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7377..7395
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7423..7649
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7650..7830
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7877..8002
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8495..8728
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8913..8957
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9132..9632
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9551..9569
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9644..10039
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10099..10216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcaccatg gcggggtgca gggggtctct gtgctgctgc tgcaggtggt
      601 gctgctgctg cggtgagcgt gagacccgca cccccgagga gctgaccatc cttggagaaa
      661 cacaggagga ggaggatgag attcttccaa ggaaagacta tgagagtttg gattatgatc
      721 gctgtatcaa tgacccttac ctggaagttt tggagaccat ggataataag aaaggtcgaa
      781 gatatgaggc ggtgaagtgg atggtggtgt ttgccattgg agtctgcact ggcctggtgg
      841 gtctctttgt ggactttttt gtgcgactct tcacccaact caagttcgga gtggtacaga
      901 catcggtgga ggagtgcagc cagaaaggct gcctcgctct gtctctcctt gaactcctgg
      961 gttttaacct cacctttgtc ttcctggcaa gcctccttgt tctcattgag ccggtggcag
     1021 caggttccgg gatacccgag gtcaaatgct atctgaatgg cgtaaaggtg ccaggaatcg
     1081 tccgtctccg gaccctgctc tgcaaggtcc ttggagtgct gttcagtgtg gctggagggc
     1141 tcttcgtggg gaaggaaggc cccatgatcc acagtggttc ggtggtggga gctggcctcc
     1201 ctcagtttca gagcatctcc ttacggaaga tccagtttaa cttcccctat ttccgaagcg
     1261 acagagacaa gagagacttt gtatcagcag gagcggctgc tggagttgct gcagctttcg
     1321 gggcgccaat cgggggtacc ttgttcagtc tagaggaggg ttcgtccttc tggaaccaag
     1381 ggctcacgtg gaaagtgctc ttttgttcca tgtctgccac cttcaccctc aacttcttcc
     1441 attctgggat tcagtttgga agctggggtt ccttccagct ccctggattg ctgaactttg
     1501 gcgagtttaa gtgctctgac tctgataaaa aatgtcatct ctggacagct atggatttgg
     1561 gtttcttcgt cgtgatgggg gtcattgggg gcctcctggg agccacattc aactgtctga
     1621 acaagaggct tgcaaagtac cgtatgcgaa acgtgcaccc gaaacctaag ctcgtcagag
     1681 tcttagagag cctccttgtg tctctggtaa ccaccgtggt ggtgtttgtg gcctcgatgg
     1741 tgttaggaga atgccgacag atgtcctctt cgagtcaaat cggtaatgac tcattccagc
     1801 tccaggtcac agaagatgtg aattcaagta tcaagacatt tttttgtccc aatgatacct
     1861 acaatgacat ggccacactc ttcttcaacc cgcaggagtc tgccatcctc cagctcttcc
     1921 accaggatgg tactttcagc cccgtcactc tggccttgtt cttcgttctc tatttcttgc
     1981 ttgcatgttg gacttacggc atttctgttc caagtggcct ttttgtgcct tctctgctgt
     2041 gtggagctgc ttttggacgt ttagttgcca atgtcctaaa aagctacatt ggattgggcc
     2101 acatctattc ggggaccttt gccctgattg gtgcagcggc tttcttgggc ggggtggtcc
     2161 gcatgaccat cagcctcacg gtcatcctga tcgagtccac caatgagatc acctacgggc
     2221 tccccatcat ggtcacactg atggtggcca aatggacagg ggactttttc aataagggca
     2281 tttatgatat ccacgtgggc ctgcgaggcg tgccgcttct ggaatgggag acagaggtgg
     2341 aaatggacaa gctgagagcc agcgacatca tggagcccaa cctgacctac gtctacccgc
     2401 acacccgcat ccagtctctg gtgagcatcc tgcgcaccac ggtccaccat gccttcccgg
     2461 tggtcacaga gaaccgcggt aacgagaagg agttcatgaa gggcaaccag ctcatcagca
     2521 acaacatcaa gttcaagaaa tccagcatcc tcacccgggc tggcgagcag cgcaaacgga
     2581 gccagtccat gaagtcctac ccatccagcg agctacggaa catgtgtgat gagcacatcg
     2641 cctctgagga gccagccgag aaggaggacc tcctgcagca gatgctggaa aggagataca
     2701 ctccctaccc caacctatac cctgaccagt ccccaagtga agactggacc atggaggagc
     2761 ggttccgccc tctgaccttc cacggcctga tccttcggtc gcagcttgtc accctgcttg
     2821 tccgaggagt ttgttactct gaaagccagt cgagcgccag ccagccgcgc ctctcctatg
     2881 ccgagatggc cgaggactac ccgcggtacc ccgacatcca cgacctggac ctgacgctgc
     2941 tcaacccgcg catgatcgtg gatgtcaccc catacatgaa cccttcgcct ttcaccgtct
     3001 cgcccaacac ccacgtctcc caagtcttca acctgttcag aacgatgggc ctgcgccacc
     3061 tgcccgtggt gaacgctgtg ggagagatcg tggggatcat cacacggcac aacctcacct
     3121 atgaatttct gcaggcccgg ctgaggcagc actaccagac catcttgcca actttcttgt
     3181 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
     3241 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     3301 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     3361 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     3421 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     3481 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     3541 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     3601 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     3661 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3721 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3781 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3841 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     3901 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     3961 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     4021 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     4081 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     4141 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     4201 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     4261 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     4321 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     4381 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     4441 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     4501 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     4561 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     4621 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4681 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4741 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4801 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4861 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4921 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4981 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     5041 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     5101 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     5161 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     5221 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     5281 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     5341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     5401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     5461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     6001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     6061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     6121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     6181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     6241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6481 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     7081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     7141 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     7201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     7261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     7321 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     7381 aaccctcact aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac
     7441 tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa
     7501 agcaccgtgc atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc
     7561 aacagacggg tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg
     7621 tatttaagtg cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct
     7681 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     7741 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     7801 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     7861 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     7921 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     7981 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     8041 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     8101 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     8161 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     8221 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     8281 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     8341 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     8401 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     8461 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     8521 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     8581 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     8641 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     8701 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     8761 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     8821 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     8881 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     8941 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     9001 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     9061 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     9121 cgaggggacc cttgcgcctt ttccaaggca gccctgggtt tgcgcaggga cgcggctgct
     9181 ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt
     9241 ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt gggccccccg gcgacgcttc
     9301 ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa
     9361 cggaagccgc acgtctcact agtaccctcg cagacggaca gcgccaggga gcaatggcag
     9421 cgcgccgacc gcgatgggct gtggccaata gcggctgctc agcagggcgc gccgagagca
     9481 gcggccggga aggggcggtg cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc
     9541 tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg cacgtcggca gtcggctccc
     9601 tcgttgaccg aatcaccgac ctctctcccc agggggtacc accatggcca agcctttgtc
     9661 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc
     9721 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt
     9781 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc
     9841 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat
     9901 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc
     9961 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc
    10021 tggttatgtg tgggagggcc tgcagctgca gtagtaagaa ttctagatct tgagacaaat
    10081 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10141 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10201 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt ggcgccggct
    10261 cgagggggcc cggggttatt aatagtaatc aattacgggg tcattagttc atagcccata
    10321 tatggagttc cg
//
