LOCUS       sequence-319649-        8937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNAB3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8937)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 8937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNAB3_OE_1
FEATURES             Location/Qualifiers
     source          1..8937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1771..1795)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1797..1838
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1880..2468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1933..1953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2351..2362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2540..2773
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2851..2972
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2888..2907)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2942..2961
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3012..3147
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3167..3186)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3168..3186)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3196..3213)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3196..3212)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3205..3227)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3354..3809
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3441..3460)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3651..3672
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3835..3939
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3940..4008,4009..4800)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4158..4177)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4971..5559
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5460..5479
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5713..5730
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5847..5868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5883..5888,5889..5906,5907..5913)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5921..5937
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5926..5948
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5945..5961
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5945..5961
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5980..6000
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5982..6000
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6028..6254
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6255..6435
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6482..6607
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7100..7333
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7518..7562
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7737..8237
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8156..8174
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8249..8644
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8704..8821
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcaccatg caggtgtcta tcgcgtgtac cgagcagaac cttcgcagcc
      601 ggagcagtga ggaccgtctg tgtggacccc ggccgggccc cgggggcggt aatggtgggc
      661 cggccggcgg ggggcacggg aatcctccgg ggggtggagg gtctggcccc aaggcccgag
      721 ctgcactggt tccccgaccc ccagcgcccg ctggggccct ccgagagagc accggccgag
      781 gcactggcat gaaatacagg aacctaggga agtctggtct tcgggtatcc tgtcttggcc
      841 taggtacctg ggtcacattt ggttctcaga tctcagatga gacagcagag gatgtgctga
      901 ctgtagccta tgagcatggt gtaaacctgt ttgacaccgc cgaagtgtac gcagcaggaa
      961 aggctgaaag aaccctaggg aacatcctca agagcaaagg ttggaggaga tcaagctatg
     1021 tcatcactac caagattttt tggggaggac aggcagaaac cgagcgaggt ttaagccgaa
     1081 agcacatcat tgagggcttg cgaggatccc tggaacgcct ccagctggga tacgtggaca
     1141 ttgtctttgc caatcgctca gaccccaact gtcctatgga ggagattgtg cgagccatga
     1201 cctatgtcat caaccagggc ctggccctat actgggggac atcccgatgg ggggctgcag
     1261 aaatcatgga ggcctactcc atggccagac agttcaatct gattcctcca gtgtgtgaac
     1321 aagcggagca ccatctgttt cagagggaga aggtggagat gcagctgcca gagctctacc
     1381 acaagattgg agttggatca gtcacttggt accctctagc ctgtggtctc attactagca
     1441 agtatgatgg gcgagtccca gatacttgca gggcctccat caagggctac cagtggctca
     1501 aggacaaagt gcagagtgaa gatggcaaga agcaacaagc caaagtcatg gaccttcttc
     1561 ctgtcgctca ccagctgggc tgcaccgtgg cccagcttgc tattgcgtgg tgtctccgca
     1621 gtgagggtgt cagctctgtc ttgctggggg tgtcgagtgc ggagcagttg atagaacacc
     1681 tgggcgcgct acaggtgctg agccagctga ccccgcagac agtgatggaa atagacgggc
     1741 tcctgggaaa caagccgcat tccaagaagt tgccaacttt cttgtacaaa gtggttggta
     1801 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
     1861 ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     1921 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     1981 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2041 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2101 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2161 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2221 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2281 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2341 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2401 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2461 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     2521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     2581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     2641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     2701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     2761 aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta tttataactt
     2821 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac
     2881 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     2941 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc
     3001 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3061 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3121 agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc
     3181 gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3241 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3301 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3361 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3421 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3481 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3541 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     3601 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3661 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3721 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3781 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     3841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     3901 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     3961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4141 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     4561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     4621 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     4681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     4741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     4801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     4861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     4921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     4981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5101 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     5521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     5581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     5641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     5701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     5761 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     5821 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     5881 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     5941 cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg
     6001 gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca
     6061 acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc
     6121 gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga
     6181 catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag
     6241 ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6301 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6361 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6421 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6481 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6541 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6601 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6661 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6721 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6781 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6841 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6901 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca
     6961 gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag
     7021 taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag
     7081 aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca
     7141 ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag
     7201 tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca
     7261 cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg
     7321 atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc
     7381 cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga
     7441 tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat
     7501 cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt
     7561 tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag
     7621 taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta
     7681 ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccttgc
     7741 gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg cgtggttccg
     7801 ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt cgcagcgtca
     7861 cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct ccgcccctaa
     7921 gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa gccgcacgtc
     7981 tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc cgaccgcgat
     8041 gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc cgggaagggg
     8101 cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc gcgcggtgtt
     8161 ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt gaccgaatca
     8221 ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag aagaatccac
     8281 cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt
     8341 cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt
     8401 tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg
     8461 caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg
     8521 acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag
     8581 tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga
     8641 gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag tattcatcca
     8701 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     8761 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     8821 tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggg
     8881 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccg
//
