LOCUS       sequence-319650-        9639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses SCNN1D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9639)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_SCNN1D_OE_1
FEATURES             Location/Qualifiers
     source          1..9639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2473..2497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2499..2540
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2582..3170
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2635..2655)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3053..3064)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3242..3475
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3553..3674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3590..3609)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3644..3663
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3714..3849
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3869..3888)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3870..3888)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3898..3915)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3898..3914)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3907..3929)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4056..4511
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4143..4162)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4353..4374
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4537..4641
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4642..4710,4711..5502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4860..4879)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5673..6261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6162..6181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6415..6432
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6549..6570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6585..6590,6591..6608,6609..6615)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6623..6639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6628..6650
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6647..6663
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6647..6663
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6682..6702
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6684..6702
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6730..6956
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6957..7137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7184..7309
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7802..8035
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8220..8264
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8439..8939
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8858..8876
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8951..9346
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9406..9523
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcaccatg gctgagcacc gaagcatgga cgggagaatg gaagcagcca
      601 cacggggggg ctctcacctc caggctgcag cccagacgcc ccccaggccg gggccaccat
      661 cagcaccacc accaccaccc aaggaggggc accaggaggg gctggtggag ctgcccgcct
      721 cgttccggga gctgctcacc ttcttctgca ccaatgccac catccacggc gccatccgcc
      781 tggtctgctc ccgcgggaac cgcctcaaga cgacgtcctg ggggctgctg tccctgggag
      841 ccctggtcgc gctctgctgg cagctggggc tcctctttga gcgtcactgg caccgcccgg
      901 tcctcatggc cgtctctgtg cactcggagc gcaagctgct cccgctggtc accctgtgtg
      961 acgggaaccc acgtcggccg agtccggtcc tccgccatct ggagctgctg gacgagtttg
     1021 ccagggagaa cattgactcc ctgtacaacg tcaacctcag caaaggcaga gccgccctct
     1081 ccgccactgt cccccgccac gagcccccct tccacctgga ccgggagatc cgtctgcaga
     1141 ggctgagcca ctcgggcagc cgggtcagag tggggttcag actgtgcaac agcacgggcg
     1201 gcgactgctt ttaccgaggc tacacgtcag gcgtggcggc tgtccaggac tggtaccact
     1261 tccactatgt ggatatcctg gccctgctgc ccgcggcatg ggaggacagc cacgggagcc
     1321 aggacggcca cttcgtcctc tcctgcagtt acgatggcct ggactgccag gcccgacagt
     1381 tccggacctt ccaccacccc acctacggca gctgctacac ggtcgatggc gtctggacag
     1441 ctcagcgccc cggcatcacc cacggagtcg gcctggtcct cagggttgag cagcagcctc
     1501 acctccctct gctgtccacg ctggccggca tcagggtcat ggttcacggc cgtaaccaca
     1561 cgcccttcct ggggcaccac agcttcagcg tccggccagg gacggaggcc accatcagca
     1621 tccgagagga cgaggtgcac cggctcggga gcccctacgg ccactgcacc gccggcgggg
     1681 aaggcgtgga ggtggagctg ctacacaaca cctcctacac caggcaggcc tgcctggtgt
     1741 cctgcttcca gcagctgatg gtggagacct gctcctgtgg ctactacctc caccctctgc
     1801 cggcgggggc tgagtactgc agctctgccc ggcaccctgc ctggggacac tgcttctacc
     1861 gcctctacca ggacctggag acccaccggc tcccctgtac ctcccgctgc cccaggccct
     1921 gcagggagtc tgcattcaag ctctccactg ggacctccag gtggccttcc gccaagtcag
     1981 ctggatggac tctggccacg ctaggtgaac aggggctgcc gcatcagagc cacagacaga
     2041 ggagcagcct ggccaaaatc aacatcgtct accaggagct caactaccgc tcagtggagg
     2101 aggcgcccgt gtactcggtg ccgcagctgc tctcggccat gggcagcctc tgcagcctgt
     2161 ggtttggggc ctccgtcctc tccctcctgg agctcctgga gctgctgctc gatgcttctg
     2221 ccctcaccct ggtgctaggc ggccgccggc tccgcagggc gtggttctcc tggcccagag
     2281 ccagccctgc ctcaggggcg tccagcatca agccagaggc cagtcagatg cccccgcctg
     2341 caggcggcac gtcagatgac ccggagccca gcgggcctca tctcccacgg gtgatgcttc
     2401 caggggttct ggcgggagtc tcagccgaag agagctgggc tgggccccag ccccttgaga
     2461 ctctggacac cttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
     2521 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
     2581 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     2641 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     2701 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     2761 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     2821 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     2881 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     2941 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     3001 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     3061 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     3121 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta
     3181 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     3241 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
     3301 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     3361 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     3421 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg
     3481 tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     3541 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     3601 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     3661 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     3721 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     3781 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     3841 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     3901 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3961 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4021 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     4081 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4141 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4201 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4261 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4321 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4381 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4441 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4501 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4561 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4621 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4741 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4801 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4861 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4981 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5041 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5101 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5161 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5221 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5281 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5341 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5401 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5461 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5521 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5581 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5641 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5701 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5761 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6301 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6361 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6421 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6481 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6541 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6601 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6661 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6721 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     6781 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     6841 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     6901 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     6961 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7021 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7081 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7141 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7201 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7261 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7321 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7381 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7441 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7501 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7561 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7621 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     7681 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     7741 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     7801 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     7861 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     7921 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     7981 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     8041 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     8101 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     8161 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     8221 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     8281 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     8341 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     8401 tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc caaggcagcc
     8461 ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg
     8521 accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta
     8581 cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc
     8641 ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag
     8701 acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg
     8761 gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt
     8821 gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc
     8881 cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg
     8941 gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     9001 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc
     9061 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     9121 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     9181 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     9241 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     9301 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta
     9361 gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta aaagaaaagg
     9421 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     9481 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     9541 cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat agtaatcaat
     9601 tacggggtca ttagttcata gcccatatat ggagttccg
//
