LOCUS       sequence-319658-        9042 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses GABRP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9042)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9042)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_GABRP_OE_1
FEATURES             Location/Qualifiers
     source          1..9042
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        134..437
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        438..641
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     592..612
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    664..688
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2009..2033)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2035..2076
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2118..2706
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2171..2191)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2589..2600)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2778..3011
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3089..3210
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3126..3145)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3180..3199
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3250..3385
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3405..3424)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3406..3424)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3434..3451)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3434..3450)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3443..3465)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3592..4047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3679..3698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3889..3910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4073..4177
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4178..4246,4247..5038)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4396..4415)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5209..5797
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5698..5717
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5951..5968
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6085..6106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6121..6126,6127..6144,6145..6151)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6159..6175
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6164..6186
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6183..6199
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6183..6199
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6218..6238
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6220..6238
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6266..6492
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6493..6673
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6720..6845
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7338..7571
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7756..7800
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7975..8475
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8394..8412
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8487..8882
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    join(8942..9042,1..17)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
       61 tcgagggggc ccggggttat taatagtaat caattacggg gtcattagtt catagcccat
      121 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      181 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      241 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      301 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      361 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      421 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      481 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      541 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      601 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actgtcggga
      661 tcaacaagtt tgtacaaaaa agcaggcacc atgaactaca gcctccactt ggccttcgtg
      721 tgtctgagtc tcttcactga gaggatgtgc atccagggga gtcagttcaa cgtcgaggtc
      781 ggcagaagtg acaagctttc cctgcctggc tttgagaacc tcacagcagg atataacaaa
      841 tttctcaggc ccaattttgg tggagaaccc gtacagatag cgctgactct ggacattgca
      901 agtatctcta gcatttcaga gagtaacatg gactacacag ccaccatata cctccgacag
      961 cgctggatgg accagcggct ggtgtttgaa ggcaacaaga gcttcactct ggatgcccgc
     1021 ctcgtggagt tcctctgggt gccagatact tacattgtgg agtccaagaa gtccttcctc
     1081 catgaagtca ctgtgggaaa caggctcatc cgcctcttct ccaatggcac ggtcctgtat
     1141 gccctcagaa tcacgacaac tgttgcatgt aacatggatc tgtctaaata ccccatggac
     1201 acacagacat gcaagttgca gctggaaagc tggggctatg atggaaatga tgtggagttc
     1261 acctggctga gagggaacga ctctgtgcgt ggactggaac acctgcggct tgctcagtac
     1321 accatagagc ggtatttcac cttagtcacc agatcgcagc aggagacagg aaattacact
     1381 agattggtct tacagtttga gcttcggagg aatgttctgt atttcatttt ggaaacctac
     1441 gttccttcca ctttcctggt ggtgttgtcc tgggtttcat tttggatctc tctcgattca
     1501 gtccctgcaa gaacctgcat tggagtgacg accgtgttat caatgaccac actgatgatc
     1561 gggtcccgca cttctcttcc caacaccaac tgcttcatca aggccatcga tgtgtacctg
     1621 gggatctgct ttagctttgt gtttggggcc ttgctagaat atgcagttgc tcactacagt
     1681 tccttacagc agatggcagc caaagatagg gggacaacaa aggaagtaga agaagtcagt
     1741 attactaata tcatcaacag ctccatctcc agctttaaac ggaagatcag ctttgccagc
     1801 attgaaattt ccagcgacaa cgttgactac agtgacttga caatgaaaac cagcgacaag
     1861 ttcaagtttg tcttccgaga aaagatgggc aggattgttg attatttcac aattcaaaac
     1921 cccagtaatg ttgatcacta ttccaaacta ctgtttcctt tgatttttat gctagccaat
     1981 gtattttact gggcatacta catgtatttt acagctttct tgtacaaagt ggttggtaag
     2041 cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg
     2101 tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     2161 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     2221 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     2281 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     2341 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     2401 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     2461 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt
     2521 cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     2581 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     2641 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     2701 ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta gccacttttt
     2761 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     2821 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2881 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     2941 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3001 aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc
     3061 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     3121 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     3181 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     3241 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3301 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     3361 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     3421 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     3481 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     3541 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     3601 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3661 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3721 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3781 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     3841 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     3901 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     3961 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4021 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4081 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4141 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4201 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4261 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     4321 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     4381 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     4441 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     4501 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     4561 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     4621 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     4681 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     4741 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     4801 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     4861 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     4921 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     4981 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     5041 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     5101 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     5161 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     5221 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     5281 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     5341 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     5401 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5461 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5521 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5581 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5641 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5701 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5761 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5821 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5881 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5941 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     6001 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     6061 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     6121 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     6181 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
     6241 acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac
     6301 atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga
     6361 ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca
     6421 tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct
     6481 cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6541 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6601 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6661 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     6721 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     6781 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     6841 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     6901 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     6961 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     7021 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     7081 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     7141 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     7201 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     7261 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     7321 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     7381 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     7441 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     7501 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     7561 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     7621 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     7681 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     7741 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     7801 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     7861 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     7921 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc
     7981 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     8041 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     8101 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     8161 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     8221 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     8281 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     8341 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     8401 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     8461 gacctctctc cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc
     8521 tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg
     8581 ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta
     8641 ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca
     8701 acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac
     8761 ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg
     8821 atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg
     8881 gcctgcagct gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca
     8941 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     9001 tagcaacaga catacaaact aaagaattac aaaaacaaat ta
//
