LOCUS       sequence-319659-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses TMEM63A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMEM63A_OE_1
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2977..3001)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3003..3044
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3086..3674
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3139..3159)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3557..3568)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3746..3979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4057..4178
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4094..4113)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4148..4167
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4218..4353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4373..4392)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4374..4392)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4402..4419)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4402..4418)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4411..4433)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4560..5015
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4647..4666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4857..4878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5041..5145
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5146..5214,5215..6006)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5364..5383)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6177..6765
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6666..6685
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6919..6936
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7053..7074
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7089..7094,7095..7112,7113..7119)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7127..7143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7132..7154
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7151..7167
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7151..7167
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7186..7206
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7188..7206
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7234..7460
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7461..7641
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7688..7813
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8306..8539
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8724..8768
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8943..9443
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9362..9380
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9455..9850
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9910..10027
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatgatg gactccccgt tcctggagct gtggcagtcc aaggcagtgt
      601 ccatcaggga gcagctggga ctcggggacc ggcccaacga ctcctattgc tacaactcgg
      661 ccaaaaacag caccgtgctc cagggggtca cctttggtgg catccccact gtcctgctca
      721 tagacgtcag ctgcttcctg ttcttaatct tggtgttttc tattataaga agaagattct
      781 gggactatgg ccgcattgcc ctggtgtcag aagcagacag cgagtccaga tttcagagat
      841 tgtcatcgac ttcctcctca ggtcaacaag actttgaaaa tgagctggga tgctgtccct
      901 ggctgactgc catcttccgt ctgcatgatg accagatcct ggaatggtgt ggggaggacg
      961 ccatccacta cctgtccttc cagaggcaca tcatcttcct gttggtggtg gtcagctttt
     1021 tgtccctgtg tgtcatcctg cctgtcaacc tctcagggga cttgctggac aaagacccgt
     1081 atagttttgg gaggacaaca atagcaaacc tacagactga caatgacctc ctttggctgc
     1141 acaccatctt tgctgtcatt tacctcttcc tcactgtggg tttcatgcgg caccacactc
     1201 agtccattaa gtacaaagag gagaacctgg tgaggcggac cctgttcatc acaggactcc
     1261 ccagagatgc caggaaggag actgtggaga gccacttccg ggacgcgtat cccacgtgtg
     1321 aggtggttga tgtgcagctg tgctacaacg tggccaaact gatctacctg tgcaaggaga
     1381 aaaagaagac tgagaagagc ctgacctatt acacaaacct gcaggtgaag acaggccagc
     1441 ggaccctcat caaccccaag ccctgtggcc agttttgctg ctgtgaagtg ctgggctgtg
     1501 agtgggaaga cgccatctct tactacacac ggatgaagga caggctgctg gagaggatca
     1561 cagaggaaga acgccacgtc caggaccagc ccctgggaat ggccttcgtc accttccagg
     1621 agaagtccat ggccacctac atcctgaaag atttcaatgc ctgcaagtgt cagagccttc
     1681 agtgcaaagg tgagccccag ccgtcctccc atagcaggga gctctatacc tccaagtgga
     1741 cagtcacctt tgctgctgac cctgaggaca tctgctggaa gaacctctct atccagggcc
     1801 tccgctggtg gctacagtgg ctgggcatca acttcaccct cttcctgggg ctatttttcc
     1861 tgaccacacc ctccatcatc ctgtccacca tggacaagtt taatgtcacc aaacccatcc
     1921 atgcgctgaa taacccgatc atcagccagt tcttccccac cctcctgctc tggtccttct
     1981 cggccctgct cccctccatt gtctactact ctacactgct ggagtctcac tggaccaagt
     2041 cgggggaaaa ccagatcatg atgaccaaag tctacatatt cttgatcttc atggtgctga
     2101 tcctgccctc cctgggtctc accagtctag attttttctt ccggtggctc tttgacaaaa
     2161 cttcctcgga ggcctccatc aggttggagt gcgtcttcct gcctgaccag ggtgccttct
     2221 ttgtgaacta tgtcatcgcc tcggccttca tcggcaatgg catggagctg ctgcggctgc
     2281 caggtctcat cctctatacc ttccgcatga tcatggccaa gacggctgct gaccgcagga
     2341 atgtcaagca gaaccaggcc ttccagtacg agtttggagc catgtatgca tggatgctgt
     2401 gtgtcttcac tgtcatcgtg gcctacagca tcacttgtcc catcatcgcg ccatttggcc
     2461 tcatctacat cctgctcaag cacatggtgg accggcacaa cctctacttc gtctacctcc
     2521 cagccaagct ggagaagggg atccactttg ccgctgtgaa ccaggccttg gcagccccca
     2581 tcctgtgcct cttctggctc tacttctttt ccttcctgcg cctgggtatg aaggcccccg
     2641 ccactctgtt caccttcctg gtgctgctgc tcaccatcct ggtctgcctg gctcacacct
     2701 gctttggatg cttcaagcac ctcagccctc tgaactacaa aacagaagag cctgcaagtg
     2761 acaaaggaag tgaggcagag gcccacatgc ccccaccgtt cacaccctac gtgcctcgga
     2821 ttctgaacgg cttggcctcg gagaggacag cactgtctcc gcagcagcag cagcagcaga
     2881 cctatggtgc catccacaac atcagcggga ctatccctgg acagtgcttg gcgcagagcg
     2941 ccacgggcag tgtggctgct gccccccagg aggcctgccc aactttcttg tacaaagtgg
     3001 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
     3061 ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt
     3121 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     3181 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     3241 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     3301 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     3361 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     3421 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     3481 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     3541 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     3601 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     3661 ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc
     3721 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3781 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3841 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3901 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3961 gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta
     4021 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     4081 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     4141 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     4201 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     4261 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     4321 tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag
     4381 tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     4441 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     4501 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     4561 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4621 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4681 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4741 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4801 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4861 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4921 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4981 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     5041 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5101 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5161 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     5221 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5281 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5341 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5401 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5461 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5521 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5581 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5641 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5701 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5761 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5821 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5881 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5941 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6001 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6061 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6121 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6181 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6241 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6301 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6361 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6421 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6481 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6541 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6601 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6661 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6721 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6781 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6841 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6901 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6961 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7021 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7081 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7141 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     7201 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7261 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7321 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     7381 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     7441 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     7501 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7561 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7621 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     7681 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     7741 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     7801 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     7861 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     7921 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     7981 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     8041 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     8101 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     8161 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     8221 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8281 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8341 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8401 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     8461 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     8521 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     8581 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     8641 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8701 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8761 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8821 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     8881 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     8941 ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg
     9001 gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg tccgttcgca
     9061 gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt cctgctccgc
     9121 ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg
     9181 cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca gcgcgccgac
     9241 cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc agcggccggg
     9301 aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc
     9361 ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc ctcgttgacc
     9421 gaatcaccga cctctctccc cagggggtac caccatggcc aagcctttgt ctcaagaaga
     9481 atccaccctc attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta
     9541 cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata
     9601 tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc
     9661 agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc
     9721 ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa
     9781 ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt
     9841 gtgggagggc ctgcagctgc agtagtaaga attctagatc ttgagacaaa tggcagtatt
     9901 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     9961 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10021 aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc tcgagggggc
    10081 ccggggttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
    10141 ccg
//
