LOCUS       sequence-319661-        9228 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNH6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9228)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNH6_OE_1
FEATURES             Location/Qualifiers
     source          1..9228
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2062..2086)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2088..2129
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2171..2759
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2224..2244)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2642..2653)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2831..3064
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3142..3263
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3179..3198)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3233..3252
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3303..3438
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3458..3477)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3459..3477)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3487..3504)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3487..3503)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3496..3518)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3645..4100
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3732..3751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3942..3963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4126..4230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4231..4299,4300..5091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4449..4468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5262..5850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5751..5770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6004..6021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6138..6159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6174..6179,6180..6197,6198..6204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6212..6228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6217..6239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6236..6252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6236..6252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6271..6291
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6273..6291
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6319..6545
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6546..6726
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6773..6898
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7391..7624
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7809..7853
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8028..8528
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8447..8465
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8540..8935
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8995..9112
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctct ctggctaact gtcgggatca acaagtttgt
      541 acaaaaaagt tggcatgccg gtccgcaggg gccacgtcgc tccccaaaac acttacctgg
      601 acaccatcat ccgcaagttc gagggccaaa gtcggaagtt cctgattgcc aatgctcaga
      661 tggagaactg cgccatcatt tactgcaacg acggcttctg cgaactcttc ggctactccc
      721 gagtggaggt gatgcagcaa ccctgcacct gcgacttcct cacaggcccc aacacaccaa
      781 gcagcgccgt gtcccgccta gcgcaggccc tgctgggggc tgaggagtgc aaggtggaca
      841 tcctctacta ccgcaaggat gcctccagct tccgctgcct ggtagatgtg gtgcccgtga
      901 agaacgagga cggggctgtc atcatgttca ttctcaactt cgaggacctg gcccagctcc
      961 tggccaagtg cagcagccgc agcttgtccc agcgcctgtt gtcccagagc ttcctgggct
     1021 ccgagggctc tcatggcagg ccaggcggac cagggccagg cacaggcagg ggcaagtaca
     1081 ggaccatcag ccagatccca cagttcacgc tcaacttcgt ggagttcaac ttggagaagc
     1141 accgctccag ctccaccacg gagattgaga tcatcgcgcc ccataaggtg gtggagcgga
     1201 cacagaacgt cactgagaag gtcacccagg tcctgtccct gggcgcggat gtgctgccgg
     1261 agtacaagct gcaggcgccg cgcatccacc gctggaccat cctgcactac agccccttca
     1321 aggccgtgtg ggactggctc atcctgctgc tggtcatcta cacggctgtc ttcacgccct
     1381 actcagccgc cttcctgctc agcgaccagg acgaatcacg gcgtggggcc tgcagctata
     1441 cctgcagtcc cctcactgtg gtggatctca tcgtggacat catgttcgtc gtggacatcg
     1501 tcatcaactt ccgcaccacc tatgtcaaca ccaatgatga ggtggtcagc cacccccgcc
     1561 gcatcgccgt ccactacttc aagggctggt tcctcattga catggtggcc gccatccctt
     1621 tcgacctcct gatcttccgc actggctccg atgagaccac aaccctgatt gggctattga
     1681 agacagcgcg gctgctgcgg ctggtgcgcg tagcacggaa gctggaccgc tactctgagt
     1741 atggggcggc tgtgctcttc ttgctcatgt gcaccttcgc gctcatagcg cactggctgg
     1801 cctgcatctg gtacgccatc ggcaatgtgg agcggcccta cctagaacac aagatcggct
     1861 ggctggacag cctgggtgtg cagcttggca agcgctacaa cggcagcgac ccagcctcgg
     1921 gcccctcggt gcaggacaag tatgtcacag ccctctactt caccttcagc agcctcacca
     1981 gcgtgggctt cggcaatgtc tcgcccaaca ccaactccga gaaggtcttc tccatctgcg
     2041 tcatgctcat cggctgtgag tgcccaactt tcttgtacaa agtggttggt aagcctatcc
     2101 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
     2161 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     2221 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2281 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     2341 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     2401 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     2461 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     2521 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     2581 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     2641 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     2701 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     2761 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     2821 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     2881 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     2941 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3001 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     3061 agcagtacgt atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     3121 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     3181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     3241 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     3301 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     3361 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     3421 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     3481 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     3541 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     3601 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     3661 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3721 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3781 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3841 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3901 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3961 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4021 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4081 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     4141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     4201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     4261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     4321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     4381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     4441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     5041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     5101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     5161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     5221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     5281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     5341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     5401 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     5461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     5521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     5581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     5641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     5701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     5761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     6001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     6061 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
     6121 agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac
     6181 tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga
     6241 aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag
     6301 ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa
     6361 cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg
     6421 aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg
     6481 gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca
     6541 taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     6601 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     6661 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     6721 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     6781 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     6841 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     6901 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     6961 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     7021 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     7081 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     7141 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     7201 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     7261 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     7321 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     7381 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     7441 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     7501 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     7561 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     7621 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     7681 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     7741 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     7801 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     7861 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     7921 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     7981 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccttg cgccttttcc
     8041 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
     8101 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     8161 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     8221 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     8281 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     8341 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     8401 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     8461 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     8521 ctccccaggg ggtaccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     8581 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     8641 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     8701 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     8761 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     8821 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     8881 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcctgca
     8941 gctgcagtag taagaattct agatcttgag acaaatggca gtattcatcc acaattttaa
     9001 aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac
     9061 agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta
     9121 ttacagggac agcagagatc cactttggcg ccggctcgag ggggcccggg gttattaata
     9181 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccg
//
