LOCUS       sequence-319671-        9702 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CACNB2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9702)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNB2_OE_1
FEATURES             Location/Qualifiers
     source          1..9702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        52..355
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        356..559
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     510..530
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    582..606
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2587..2611)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2613..2654
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2696..3284
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2749..2769)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3167..3178)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3356..3589
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3667..3788
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3704..3723)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3758..3777
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3828..3963
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3983..4002)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3984..4002)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4012..4029)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4012..4028)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4021..4043)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4170..4625
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4257..4276)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4467..4488
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4651..4755
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4756..4824,4825..5616)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4974..4993)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5787..6375
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6276..6295
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6529..6546
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6663..6684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6699..6704,6705..6722,6723..6729)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6737..6753
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6742..6764
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6761..6777
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6761..6777
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6796..6816
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6798..6816
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6844..7070
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7071..7251
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7298..7423
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7916..8149
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8334..8378
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8553..9053
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8972..8990
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9065..9460
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9520..9637
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
       61 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      121 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      181 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      241 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      301 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      361 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      421 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      481 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      541 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
      601 caggcaccat ggtccaaagg gacatgtcca agtcgcctcc cacagcggcg gcggcggtgg
      661 cgcaggagat ccagatggaa ctgctagaga acgtggctcc cgcgggggcg ctcggagccg
      721 ccgcacagtc atatggaaaa ggagccagaa ggaaaaacag atttaaagga tctgatggaa
      781 gcacgtcatc tgatactacc tcaaatagtt ttgttcgcca gggttcggca gactcctaca
      841 ctagccgtcc atccgattcc gatgtatctc tggaggagga ccgggaggca gtgcgcagag
      901 aagcggagcg gcaggcccag gcacagttgg aaaaagcaaa gacaaagccc gttgcatttg
      961 cggttcggac aaatgtcagc tacagtgcgg cccatgaaga tgatgttcca gtgcctggca
     1021 tggccatctc attcgaagca aaagattttc tgcatgttaa ggaaaaattt aacaatgact
     1081 ggtggatagg gcgattggta aaagaaggct gtgaaatcgg attcattcca agcccagtca
     1141 aactagaaaa catgaggctg cagcatgaac agagagccaa gcaagggaaa ttctactcca
     1201 gtaaatcagg aggaaattca tcatccagtt tgggtgacat agtacctagt tccagaaaat
     1261 caacacctcc atcatctgct atagacatag atgctactgg cttagatgca gaagaaaatg
     1321 atattccagc aaaccaccgc tcccctaaac ccagtgcaaa cagtgtaacg tcaccccact
     1381 ccaaagagaa aagaatgccc ttctttaaga agacagagca cactcctccg tatgatgtgg
     1441 taccttccat gcgaccagtg gtcctagtgg gcccttctct gaagggctac gaggtcacag
     1501 atatgatgca aaaagcgctg tttgattttt taaaacacag atttgaaggg cggatatcca
     1561 tcacaagggt caccgctgac atctcgcttg ccaaacgctc ggtattaaac aatcccagta
     1621 agcacgcaat aatagaaaga tccaacacaa ggtcaagctt agcggaagtt cagagtgaaa
     1681 tcgaaaggat ttttgaactt gcaagaacat tgcagttggt ggtccttgac gcggatacaa
     1741 ttaatcatcc agctcaactc agtaaaacct ccttggcccc tattatagta tatgtaaaga
     1801 tttcttctcc taaggtttta caaaggttaa taaaatctcg agggaaatct caagctaaac
     1861 acctcaacgt ccagatggta gcagctgata aactggctca gtgtcctcca gagctgttcg
     1921 atgtgatctt ggatgagaac cagcttgagg atgcctgtga gcaccttgcc gactatctgg
     1981 aggcctactg gaaggccacc catcctccca gcagtagcct ccccaaccct ctccttagcc
     2041 gtacattagc cacttcaagt ctgcctctta gccccaccct agcctctaat tcacagggtt
     2101 ctcaaggtga tcagaggact gatcgctccg ctcctatccg ttctgcttcc caagctgaag
     2161 aagaacctag tgtggaacca gtcaagaaat cccagcaccg ctcttcctcc tcagccccac
     2221 accacaacca tcgcagtggg acaagtcgcg gcctctccag gcaagagaca tttgactcgg
     2281 aaacccagga gagtcgagac tctgcctacg tagagccaaa ggaagattat tcccatgacc
     2341 acgtggacca ctatgcctca caccgtgacc acaaccacag agacgagacc cacgggagca
     2401 gtgaccacag acacagggag tcccggcacc gttcccggga cgtggatcga gagcaggacc
     2461 acaacgagtg caacaagcag cgcagccgtc ataaatccaa ggatcgctac tgtgaaaagg
     2521 atggagaagt gatatcaaaa aaacggaatg aggctgggga gtggaacagg gatgtttaca
     2581 tccgccaaac agctttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
     2641 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
     2701 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2761 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2821 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2881 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2941 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3001 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3061 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3121 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3181 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3241 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca
     3301 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     3361 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     3421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     3481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     3541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt
     3601 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
     3661 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3721 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3781 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
     3841 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     3901 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     3961 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     4021 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4081 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4141 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     4201 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4261 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4321 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4381 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4441 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4501 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4561 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4621 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4681 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4741 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4801 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4861 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4921 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4981 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     5041 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5101 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5161 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5221 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5281 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5341 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5401 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5461 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5521 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5581 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5641 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5701 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5761 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5821 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5881 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5941 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     6001 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6061 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6121 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6181 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6241 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6301 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6361 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6421 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6481 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6541 agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     6601 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
     6661 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
     6721 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
     6781 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagct
     6841 taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac
     6901 atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg
     6961 atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga accactgaat
     7021 tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct
     7081 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7141 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7201 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     7261 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     7321 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     7381 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     7441 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     7501 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     7561 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     7621 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     7681 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     7741 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     7801 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     7861 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     7921 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     7981 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     8041 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     8101 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     8161 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     8221 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     8281 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     8341 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     8401 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     8461 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     8521 ttcagaccca cctcccaacc ccgaggggac ccttgcgcct tttccaaggc agccctgggt
     8581 ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc gccgaccctg
     8641 ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc gctacccttg
     8701 tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg
     8761 cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc gcagacggac
     8821 agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat agcggctgct
     8881 cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg gggtgtgggg
     8941 cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag cctccggagc
     9001 gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc cagggggtac
     9061 caccatggcc aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac
     9121 aatcaacagc atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga
     9181 cggccgcatc ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact
     9241 cgtggtgctg ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat
     9301 cggaaatgag aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga
     9361 tctgcatcct gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg
     9421 gattcgtgaa ttgctgccct ctggttatgt gtgggagggc ctgcagctgc agtagtaaga
     9481 attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     9541 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     9601 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     9661 agatccactt tggcgccggc tcgagggggc ccggggttat ta
//
