LOCUS       sequence-319672-        9174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CACNB3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9174)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNB3_OE_1
FEATURES             Location/Qualifiers
     source          1..9174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        300..503
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     454..474
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    526..550
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2003..2027)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2029..2070
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2112..2700
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2165..2185)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2583..2594)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2772..3005
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3083..3204
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3120..3139)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3174..3193
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3244..3379
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3399..3418)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3400..3418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3428..3445)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3428..3444)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3437..3459)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3586..4041
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3673..3692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3883..3904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4067..4171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4172..4240,4241..5032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4390..4409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5203..5791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5692..5711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5945..5962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6079..6100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6115..6120,6121..6138,6139..6145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6153..6169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6158..6180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6177..6193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6177..6193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6212..6232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6214..6232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6260..6486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6487..6667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6714..6839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7332..7565
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7750..7794
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7969..8469
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8388..8406
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8481..8876
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8936..9053
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9170..9174,1..299)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
       61 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      121 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      181 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      241 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      301 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      361 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      421 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      481 gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa
      541 aaagttggca tgtatgacga ctcctacgtg cccgggtttg aggactcgga ggcgggttca
      601 gccgactcct acaccagccg cccatctctg gactcagacg tctccctgga ggaggaccgg
      661 gagagtgccc ggcgtgaagt agagagccag gctcagcagc agctcgaaag ggccaagcac
      721 aaacctgtgg catttgcggt gaggaccaat gtcagctact gtggcgtact ggatgaggag
      781 tgcccagtcc agggctctgg agtcaacttt gaggccaaag attttctgca cattaaagag
      841 aagtacagca atgactggtg gatcgggcgg ctagtgaaag agggcgggga catcgccttc
      901 atccccagcc cccagcgcct ggagagcatc cggctcaaac aggagcagaa ggccaggaga
      961 tctgggaacc cttccagcct gagtgacatt ggcaaccgac gctcccctcc gccatctcta
     1021 gccaagcaga agcaaaagca ggcggaacat gttcccccat atgacgtggt gccctccatg
     1081 cggcctgtgg tgctggtggg accatctctg aaaggttatg aggtcacaga catgatgcag
     1141 aaggctctct tcgacttcct caaacacaga tttgatggca ggatctccat cacccgagtc
     1201 acagccgacc tctccctggc aaagcgatct gtgctcaaca atccgggcaa gaggaccatc
     1261 attgagcgct cctctgcccg ctccagcatt gcggaagtgc agagtgagat cgagcgcata
     1321 tttgagctgg ccaaatccct gcagctagta gtgttggacg ctgacaccat caaccaccca
     1381 gcacagctgg ccaagacctc gctggccccc atcatcgtct ttgtcaaagt gtcctcacca
     1441 aaggtactcc agcgtctcat tcgctcccgg gggaagtcac agatgaagca cctgaccgta
     1501 cagatgatgg catatgataa gctggttcag tgcccaccgg agtcatttga tgtgattctg
     1561 gatgagaacc agctggagga tgcctgtgag cacctggctg agtacctgga ggtttactgg
     1621 cgggccacgc accacccagc ccctggcccc ggacttctgg gtcctcccag tgccatcccg
     1681 ggacttcaga accagcagct gctgggggag cgtggcgagg agcactcccc ccttgagcgg
     1741 gacagcttga tgccctctga tgaggccagc gagagctccc gccaagcctg gacaggatct
     1801 tcacagcgta gctcccgcca cctggaggag gactatgcag atgcctacca ggacctgtac
     1861 cagcctcacc gccaacacac ctcggggctg cctagtgcta acgggcatga cccccaagac
     1921 cggcttctag cccaggactc agagcacaac cacagtgacc ggaactggca gcgcaaccgg
     1981 ccttggccca aggatagcta ctgcccaact ttcttgtaca aagtggttgg taagcctatc
     2041 cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc
     2101 gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2161 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2221 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2281 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2341 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2401 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2461 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2521 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2581 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2641 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2701 gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     2761 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
     2821 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     2881 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     2941 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     3001 tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     3061 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     3121 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     3181 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     3241 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     3301 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     3361 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     3421 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     3481 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     3541 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     3601 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     3661 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     3721 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     3781 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     3841 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     3901 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     3961 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     4021 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     4081 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     4141 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     4201 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     4261 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     4321 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     4381 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     4441 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     4501 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     4561 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     4621 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     4681 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     4741 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     4801 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     4861 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     4921 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     4981 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     5041 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     5101 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5161 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5221 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5281 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5341 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5401 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5461 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5521 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5581 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5641 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5701 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     5761 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     5821 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     5881 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     5941 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     6001 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     6061 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     6121 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     6181 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     6241 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     6301 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     6361 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     6421 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     6481 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     6541 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     6601 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     6661 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     6721 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     6781 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     6841 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     6901 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     6961 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     7021 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     7081 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     7141 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     7201 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     7261 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     7321 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     7381 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     7441 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     7501 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     7561 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     7621 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     7681 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     7741 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     7801 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     7861 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     7921 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggaccctt gcgccttttc
     7981 caaggcagcc ctgggtttgc gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc
     8041 agcggcgccg accctgggtc tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc
     8101 ttcgccgcta cccttgtggg ccccccggcg acgcttcctg ctccgcccct aagtcgggaa
     8161 ggttccttgc ggttcgcggc gtgccggacg tgacaaacgg aagccgcacg tctcactagt
     8221 accctcgcag acggacagcg ccagggagca atggcagcgc gccgaccgcg atgggctgtg
     8281 gccaatagcg gctgctcagc agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg
     8341 gaggcggggt gtggggcggt agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc
     8401 tgcaagcctc cggagcgcac gtcggcagtc ggctccctcg ttgaccgaat caccgacctc
     8461 tctccccagg gggtaccacc atggccaagc ctttgtctca agaagaatcc accctcattg
     8521 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
     8581 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
     8641 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
     8701 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
     8761 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
     8821 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc
     8881 agctgcagta gtaagaattc tagatcttga gacaaatggc agtattcatc cacaatttta
     8941 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
     9001 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
     9061 attacaggga cagcagagat ccactttggc gccggctcga gggggcccgg ggttattaat
     9121 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gtta
//
