LOCUS       sequence-319673-        9285 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CACNB4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CACNB4_OE_1
FEATURES             Location/Qualifiers
     source          1..9285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        76..379
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        380..583
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     534..554
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    606..630
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2194..2218)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2220..2261
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2303..2891
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2356..2376)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2774..2785)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2963..3196
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3274..3395
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3311..3330)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3365..3384
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3435..3570
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3590..3609)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3591..3609)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3619..3636)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3619..3635)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3628..3650)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3777..4232
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3864..3883)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4074..4095
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4258..4362
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4363..4431,4432..5223)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4581..4600)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5394..5982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5883..5902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6136..6153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6270..6291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6306..6311,6312..6329,6330..6336)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6344..6360
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6349..6371
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6368..6384
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6368..6384
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6403..6423
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6405..6423
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6451..6677
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6678..6858
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6905..7030
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7523..7756
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7941..7985
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8160..8660
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8579..8597
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8672..9067
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9127..9244
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctcgagggg gcccggggtt attaatagta atcaattacg gggtcattag ttcatagccc
       61 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
      121 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
      181 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
      241 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
      301 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
      361 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
      421 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
      481 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
      541 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctctggc taactgtcgg
      601 gatcaacaag tttgtacaaa aaagttggca ccatgtcctc ctcctcctac gccaagaacg
      661 ggaccgcgga cgggccgcac tcccccacct cgcaggtggc ccgaggcacc acaacccgga
      721 ggagcaggtt gaaaagatcc gatggcagca ccacttcgac cagcttcatc ctcagacagg
      781 gttcagcgga ttcctacaca agcaggccgt ctgactccga tgtctctttg gaagaggacc
      841 gggaagcaat tcgacaggag agagaacagc aagcagctat ccagcttgag agagcaaagt
      901 ccaaacctgt agcatttgcc gtgaagacaa atgtgagcta ctgcggcgcc ctggacgagg
      961 atgtgcctgt tccaagcaca gctatctcct ttgatgctaa agactttcta catattaaag
     1021 agaaatataa caatgattgg tggataggaa ggctggtgaa agagggctgt gaaattggct
     1081 tcattccaag tccactcaga ttggagaaca tacggatcca gcaagaacaa aaaagaggac
     1141 gttttcacgg agggaaatca agtggaaatt cttcttcaag tcttggagaa atggtatctg
     1201 ggacattccg agcaactccc acatcaacag caaaacagaa gcaaaaagtg acggagcaca
     1261 ttcctcctta cgatgttgta ccgtcaatgc gtccggtggt gttagtgggg ccgtcactga
     1321 aaggttacga ggtaacagac atgatgcaga aagccctctt tgatttcctg aagcacaggt
     1381 ttgatgggag gatttcaata acgagagtga cagctgacat ttctcttgct aagaggtctg
     1441 tcctaaataa tcccagcaag agagcaataa ttgaacgttc gaacacccgg tccagcttag
     1501 cggaagtaca aagtgaaatt gaaagaatct ttgagttggc aagatctttg caactggttg
     1561 ttcttgatgc agacaccatc aatcacccag cacaacttat aaagacttcc ttagcaccaa
     1621 ttattgttca tgtaaaagtc tcatctccaa aggttttaca gcggttgatt aaatctagag
     1681 gaaagtcaca aagtaaacac ttgaatgttc aactggtggc agctgataaa cttgcacaat
     1741 gccccccaga aatgtttgat gttatattgg atgaaaatca gcttgaggat gcatgtgaac
     1801 atctagggga gtacctggag gcgtactggc gtgccaccca cacaaccagt agcacaccca
     1861 tgaccccgct gctgggaagg aatttgggct ccacggcact ctcaccatat cccacagcaa
     1921 tttctgggtt acagagtcag cgaatgaggc acagcaacca ctccacagag aactctccaa
     1981 ttgaaagacg aagtctaatg acctctgatg aaaattatca caatgaaagg gctcggaaga
     2041 gtaggaaccg cttgtcttcc agttctcagc atagccgaga tcattaccct cttgtggaag
     2101 aagattaccc tgactcatac caggacactt acaaacccca taggaaccga ggatcacctg
     2161 ggggatatag ccatgactcc cgacataggc ttttgccaac tttcttgtac aaagtggttg
     2221 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
     2281 accggtggcg cgttaagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac
     2341 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     2401 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     2461 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     2521 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     2581 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     2641 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     2701 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     2761 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     2821 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     2881 cgcctccccg cgtcgacttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     2941 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     3001 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3061 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3121 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3181 tggaaaatct ctagcagtac gtatagtagt tcatgtcatc ttattattca gtatttataa
     3241 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     3301 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     3361 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     3421 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     3481 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     3541 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     3601 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3661 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3721 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     3781 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     3841 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     3901 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     3961 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4021 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4081 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4141 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4201 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     4261 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4321 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4381 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4441 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4501 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4561 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4621 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4681 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4741 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4801 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4861 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4921 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4981 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5041 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5101 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5161 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5221 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5281 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5341 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5521 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5761 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6241 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6301 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6361 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
     6421 agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt
     6481 gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat
     6541 gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc
     6601 tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc
     6661 tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     6721 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     6781 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     6841 tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga
     6901 ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg
     6961 gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg
     7021 cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag
     7081 gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga
     7141 acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg
     7201 acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt
     7261 agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga
     7321 caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct
     7381 tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat aaatataaag
     7441 tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga
     7501 gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa
     7561 gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta
     7621 tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac
     7681 tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa
     7741 aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg
     7801 tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct
     7861 ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag
     7921 aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa
     7981 gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga
     8041 tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag
     8101 ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg aggggaccct
     8161 tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt
     8221 ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg
     8281 tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc
     8341 taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac
     8401 gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc
     8461 gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag
     8521 gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt
     8581 gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa
     8641 tcaccgacct ctctccccag ggggtaccac catggccaag cctttgtctc aagaagaatc
     8701 caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg aagactacag
     8761 cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca atgtatatca
     8821 ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg ctgcggcagc
     8881 tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct tgagcccctg
     8941 cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca tagtgaagga
     9001 cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg gttatgtgtg
     9061 ggagggcctg cagctgcagt agtaagaatt ctagatcttg agacaaatgg cagtattcat
     9121 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     9181 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     9241 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccg
//
