LOCUS       sequence-319678-        3855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
            Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, 
            Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, 
            Kyratsous CA, Dudkina NV, Shen K, Luban J
  TITLE     Structural and Functional Analysis of the D614G SARS-CoV-2 Spike 
            Protein Variant.
  JOURNAL   Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032.
  PUBMED    32991842
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020 
            Sep 15. pii: S0092-8674(20)31229-0. doi: 
            10.1016/j.cell.2020.09.032."
COMMENT     Sequence Label: pCAGGS D614G soluble Spike
FEATURES             Location/Qualifiers
     source          1..3855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3724..3741
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             3835..3852
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt gttgtgctgt
       61 ctcatcattt tggcaaaggc caccatgttc gtgtttctgg tgctgctgcc tctggtgtcc
      121 agccagtgtg tgaacctgac cacaagaacc cagctgcctc cagcctacac caacagcttt
      181 accagaggcg tgtactaccc cgacaaggtg ttcagatcca gcgtgctgca ctctacccag
      241 gacctgttcc tgcctttctt cagcaacgtg acctggttcc acgccatcca cgtgtccggc
      301 accaatggca ccaagagatt cgacaacccc gtgctgccct tcaacgacgg ggtgtacttt
      361 gccagcaccg agaagtccaa catcatcaga ggctggatct tcggcaccac actggacagc
      421 aagacccaga gcctgctgat cgtgaacaac gccaccaacg tggtcatcaa agtgtgcgag
      481 ttccagttct gcaacgaccc cttcctgggc gtctactatc acaagaacaa caagagctgg
      541 atggaaagcg agttccgggt gtacagcagc gccaacaact gcaccttcga gtacgtgtcc
      601 cagcctttcc tgatggacct ggaaggcaag cagggcaact tcaagaacct gcgcgagttc
      661 gtgttcaaga acatcgacgg ctacttcaag atctacagca agcacacccc tatcaacctc
      721 gtgcgggatc tgcctcaggg cttctctgct ctggaacccc tggtggatct gcccatcggc
      781 atcaacatca cccggtttca gacactgctg gccctgcaca gaagctacct gacacctggc
      841 gatagcagca gcggatggac agctggtgcc gccgcttact atgtgggcta cctgcagcct
      901 agaacctttc tgctgaagta caacgagaac ggcaccatca ccgacgccgt ggattgtgct
      961 ctggatcctc tgagcgagac aaagtgcacc ctgaagtcct tcaccgtgga aaagggcatc
     1021 taccagacca gcaacttccg ggtgcagccc accgaatcca tcgtgcggtt ccccaatatc
     1081 accaatctgt gccccttcgg cgaggtgttc aatgccacca gattcgcctc tgtgtacgcc
     1141 tggaaccgga agcggatcag caattgcgtg gccgactact ccgtgctgta caactccgcc
     1201 agcttcagca ccttcaagtg ctacggcgtg tcccctacca agctgaacga cctgtgcttc
     1261 acaaacgtgt acgccgacag cttcgtgatc cggggagatg aagtgcggca gattgcccct
     1321 ggacagacag gcaagatcgc cgactacaac tacaagctgc ccgacgactt caccggctgt
     1381 gtgattgcct ggaacagcaa caacctggac tccaaagtcg gcggcaacta caattacctg
     1441 taccggctgt tccggaagtc caatctgaag cccttcgagc gggacatctc caccgagatc
     1501 tatcaggccg gcagcacccc ttgtaacggc gtggaaggct tcaactgcta cttcccactg
     1561 cagtcctacg gctttcagcc cacaaatggc gtgggctatc agccctacag agtggtggtg
     1621 ctgagcttcg aactgctgca tgcccctgcc acagtgtgcg gccctaagaa aagcaccaat
     1681 ctcgtgaaga acaaatgcgt gaacttcaac ttcaacggcc tgaccggcac cggcgtgctg
     1741 acagagagca acaagaagtt cctgccattc cagcagtttg gccgggatat cgccgatacc
     1801 acagacgccg ttagagatcc ccagacactg gaaatcctgg acatcacccc ttgcagcttc
     1861 ggcggagtgt ctgtgatcac ccctggcacc aacaccagca atcaggtggc agtgctgtac
     1921 cagggcgtga actgtaccga agtgcccgtg gccattcacg ccgatcagct gacacctaca
     1981 tggcgggtgt actccaccgg cagcaatgtg tttcagacca gagccggctg tctgatcgga
     2041 gccgagcacg tgaacaatag ctacgagtgc gacatcccca tcggcgctgg catctgtgcc
     2101 agctaccaga cacagacaaa cagccccgcc tctgtggcca gccagagcat cattgcctac
     2161 acaatgtctc tgggcgccga gaacagcgtg gcctactcca acaactctat cgctatcccc
     2221 accaacttca ccatcagcgt gaccacagag atcctgcctg tgtccatgac caagaccagc
     2281 gtggactgca ccatgtacat ctgcggcgat tccaccgagt gctccaacct gctgctgcag
     2341 tacggcagct tctgcaccca gctgaataga gccctgacag ggatcgccgt ggaacaggac
     2401 aagaacaccc aagaggtgtt cgcccaagtg aagcagatct acaagacccc tcctatcaag
     2461 gacttcggcg gcttcaattt cagccagatt ctgcccgatc ctagcaagcc cagcaagcgg
     2521 agcttcatcg aggacctgct gttcaacaaa gtgacactgg ccgacgccgg cttcatcaag
     2581 cagtatggcg attgtctggg cgacattgcc gccagggatc tgatttgcgc ccagaagttt
     2641 aacggactga cagtgctgcc tcctctgctg accgatgaga tgatcgccca gtacacatct
     2701 gccctgctgg ccggcacaat cacaagcggc tggacatttg gagctggcgc cgctctgcag
     2761 atcccctttg ctatgcagat ggcctaccgg ttcaacggca tcggagtgac ccagaatgtg
     2821 ctgtacgaga accagaagct gatcgccaac cagttcaaca gcgccatcgg caagatccag
     2881 gacagcctga gcagcacagc aagcgccctg ggaaagctgc aggacgtggt caaccagaat
     2941 gcccaggcac tgaacaccct ggtcaagcag ctgtcctcca acttcggcgc catcagctct
     3001 gtgctgaacg atatcctgag cagactggac cctcctgaag ccgaggtgca gatcgacaga
     3061 ctgatcaccg gaaggctgca gtccctgcag acctacgtta cccagcagct gatcagagcc
     3121 gccgagatta gagcctctgc caatctggcc gccaccaaga tgtctgagtg tgtgctgggc
     3181 cagagcaaga gagtggactt ttgcggcaag ggctaccacc tgatgagctt ccctcagtct
     3241 gcccctcacg gcgtggtgtt tctgcacgtg acatacgtgc ccgctcaaga gaagaatttc
     3301 accaccgctc cagccatctg ccacgacggc aaagcccact ttcctagaga aggcgtgttc
     3361 gtgtccaacg gcacccattg gttcgtgacc cagcggaact tctacgagcc ccagatcatc
     3421 accaccgaca acaccttcgt gtctggcaac tgcgacgtcg tgatcggcat tgtgaacaat
     3481 accgtgtacg accctctgca gcccgagctg gacagcttca aagaggaact ggataagtac
     3541 tttaagaacc acacaagccc cgacgtggac ctgggcgata tcagcggaat caatgccagc
     3601 gtcgtgaaca tccagaaaga gatcgaccgg ctgaacgagg tggccaagaa tctgaacgag
     3661 agcctgatcg acctgcaaga actggggaag tacgagcagt acatcaagtg gcccagcggc
     3721 cgcttggtcc cacgtggctc acccggatct ggatacatcc cggaggcccc tagggacggt
     3781 caagcttacg tgagaaagga cggcgaatgg gttctgctgt cgaccttctt gggacatcat
     3841 catcatcatc actaa
//
