LOCUS       sequence-319684-        2578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Intron sequence from S. lycopersicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2578)
  TITLE     Orzaez lab plasmids
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pUPD2 hpIntron (GB1281)
FEATURES             Location/Qualifiers
     source          1..2578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      519..576
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(600..617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(771..1359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(851..870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1542..1636)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1657..2316)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2231..2250
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2478..2496
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2500..2543)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2546..2567
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ctcgagccgt acacaggtgt gacaatagtt gttatttcag ttctctctta cttttagtgt
       61 agctgtaata tagtttggtt atgagtgaac ttgtttgatg atttggttga ccagcttgtt
      121 gcaattggaa catatgaact tgttatattc acaccaagac atggcatatg acttgtccgc
      181 acagctgtct ttagcgtact cccgtatagg ttattttgta ccatatggta aaggtgtggg
      241 cactctagaa tacattcgtt tgagctggaa caataatcaa gagaatctgt tcaatagtta
      301 gttcacttaa ggtgatgtct gtaatatgta ggatgggaaa atagggtggt gctgtagtgt
      361 tccttgctct gttttatttt gtatcttgta actctgttgg agtcatttga ccatatttat
      421 cagtcgtcga ggtgaatcag tgagtgtttg acattgacat gatctgtagg ttgtcatttc
      481 gtgagaccac tcatcgcgac tagtagcggc cgctgcagtc cggcaaaaaa gggcaaggtg
      541 tcaccaccct gccctttttc tttaaaaccg aaaagattac ttcgcgttat gcaggcttcc
      601 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      661 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      721 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccacagg
      781 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      841 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      901 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      961 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1021 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1081 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     1141 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1201 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1261 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1321 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1501 agtatatatg agtaaacttg gtctgacagc tcgaggcttg gattctcacc aataaaaaac
     1561 gcccggcggc aaccgagcgt tctgaacaaa tccagatgga gttctgaggt cattactgga
     1621 tctatcaaca ggagtccaag cgagctcgat atcaaattac gccccgccct gccactcgtc
     1681 gcagtactgt tgtaattcat taagcattct gccgacatgg aagccatcac aaacggcatg
     1741 atgaacctga atcgccagcg gcatcagcac cttgtcgcct tgcgtataat atttgcccat
     1801 ggtgaaaacg ggggcgaaga agttgtccat attggccacg tttaaatcaa aactggtgaa
     1861 actcacccag ggattggctg atacgaaaaa catattctca ataaaccctt tagggaaata
     1921 ggccaggttt tcaccgtaac acgccacatc ttgcgaatat atgtgtagaa actgccggaa
     1981 atcgtcgtgg tattcactcc agagtgatga aaacgtttca gtttgctcat ggaaaacggt
     2041 gtaacaaggg tgaacactat cccatatcac cagctcaccg tctttcattg ccatacgaaa
     2101 ttccggatga gcattcatca ggcgggcaag aatgtgaata aaggccggat aaaacttgtg
     2161 cttatttttc tttacggtct ttaaaaaggc cgtaatatcc agctgaacgg tctggttata
     2221 ggtacattga gcaactgact gaaatgcctc aaaatgttct ttacgatgcc attgggatat
     2281 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
     2341 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2401 acctcttacg tgcccgatca actcgagtgc cacctgacgt ctaagaaacc attattatca
     2461 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct
     2521 tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagcga tgagtggt
//
