LOCUS       sequence-319705-        9030 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses KCNJ14.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9030)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_KCNJ14_OE_1
FEATURES             Location/Qualifiers
     source          1..9030
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        73..376
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        377..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     531..551
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    603..627
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1936..1960)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1962..2003
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2045..2633
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2098..2118)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2516..2527)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2705..2938
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3016..3137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3053..3072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3107..3126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3177..3312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3332..3351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3333..3351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3361..3378)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3361..3377)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3370..3392)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3519..3974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3606..3625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3816..3837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4000..4104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4105..4173,4174..4965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4323..4342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5136..5724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5625..5644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5878..5895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6012..6033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6048..6053,6054..6071,6072..6078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6086..6102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6091..6113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6110..6126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6110..6126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6145..6165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6147..6165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6193..6419
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6420..6600
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6647..6772
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7265..7498
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7683..7727
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7902..8402
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8321..8339
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8414..8809
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8869..8986
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cgagggggcc cggggttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      421 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      481 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      541 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctgtcgggat
      601 caacaagttt gtacaaaaaa gttggcatgg gcctggccag ggccctacgc cgcctcagcg
      661 gcgccctgga ttcgggagac agccgggcgg gcgatgaaga ggaggccggg cccgggttgt
      721 gccgcaacgg gtgggcgccg gcaccggtgc agtcacccgt gggccggcgc cgcggtcgct
      781 tcgtcaagaa agacgggcac tgcaacgtgc gtttcgtaaa cctgggtggc cagggcgcgc
      841 gctacctgag cgacctgttc accacatgcg tggacgtgcg ctggcgctgg atgtgcctgc
      901 tcttctcctg ctccttcctc gcctcctggc tgctcttcgg cctggccttc tggctcattg
      961 cctcgctgca cggcgacctg gccgccccgc caccgcccgc gccctgcttc tcacacgtgg
     1021 ccagcttcct ggccgccttc ctcttcgcgc tggagacgca gacgtccatc ggctacggcg
     1081 tgcgcagcgt caccgaggag tgcccggccg ctgtggccgc cgtggtgctg cagtgcattg
     1141 ccggctgcgt gctcgacgcc ttcgtcgtgg gtgctgtcat ggccaagatg gccaaaccca
     1201 agaagcgcaa cgagacgctg gtcttcagcg agaacgccgt cgtggcgctg cgcgaccacc
     1261 gcctctgcct catgtggcgc gtcggcaacc tgcgccgcag ccacctggtc gaggcccacg
     1321 tgcgtgccca gctgctgcag ccccgtgtga ccccagaggg tgagtacatc ccgctggacc
     1381 accaggatgt ggatgtgggc tttgatggag gcaccgatcg tatcttcctc gtgtccccca
     1441 tcaccatcgt ccatgagatc gactctgcca gtcctctgta tgagctagga cgtgccgagc
     1501 tggccagggc tgactttgag ctggtggtca ttctcgaggg gatggttgag gccacagcca
     1561 tgaccacaca gtgtcgctcg tcctacctcc ctggtgaact gctctggggc catcgttttg
     1621 agccagttct cttccagcgt ggctcccagt atgaggtcga ctatcgccac ttccatcgca
     1681 cttatgaggt cccagggaca ccggtctgca gtgctaagga gctggatgaa cgggcagagc
     1741 aggcttccca cagcctcaag tctagtttcc ccggctctct gactgcattt tgttatgaga
     1801 atgaacttgc tctgagctgc tgccaggagg aagatgagga cgatgagact gaggaaggga
     1861 atggggtgga aacagaagat ggggctgcta gcccccgagt tctcacacca accctggcgc
     1921 tgaccctgcc tccatgccca actttcttgt acaaagtggt tggtaagcct atccctaacc
     1981 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
     2041 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2101 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2161 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2221 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2281 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2341 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2401 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     2461 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2521 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     2581 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     2641 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     2701 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     2761 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     2821 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     2881 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     2941 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     3001 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3121 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     3181 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3241 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3301 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     3361 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3421 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3481 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     3541 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     3601 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     3661 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     3721 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     3781 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     3841 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     3901 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     3961 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4021 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4081 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4141 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4201 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4261 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4321 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4381 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4441 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4501 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4561 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4621 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     4681 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     4741 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     4801 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     4861 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     4921 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     4981 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5041 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5101 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5161 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5221 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5281 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5341 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5401 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5461 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5521 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5581 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     5641 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     5701 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     5761 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     5821 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     5881 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     5941 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6001 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6061 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6121 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     6181 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     6241 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     6301 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     6361 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     6421 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6481 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6541 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6601 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6661 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6721 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6781 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6841 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6901 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6961 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     7021 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     7081 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     7141 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     7201 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     7261 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     7321 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     7381 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     7441 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     7501 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     7561 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     7621 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     7681 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     7741 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     7801 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     7861 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cttgcgcctt ttccaaggca
     7921 gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg
     7981 ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg
     8041 ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct
     8101 tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg
     8161 cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata
     8221 gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg
     8281 ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc
     8341 ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc
     8401 agggggtacc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     8461 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
     8521 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     8581 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     8641 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     8701 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     8761 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggcc tgcagctgca
     8821 gtagtaagaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
     8881 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     8941 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     9001 ggacagcaga gatccacttt ggcgccggct
//
