LOCUS       sequence-319709-       10218 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses TMEM63B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10218)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 10218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMEM63B_OE_1
FEATURES             Location/Qualifiers
     source          1..10218
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        56..359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        360..563
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     514..534
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..610
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1968..1985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(3107..3131)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3133..3174
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3216..3804
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3269..3289)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3687..3698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3876..4109
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4187..4308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4224..4243)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4278..4297
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4348..4483
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4503..4522)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4504..4522)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4532..4549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4532..4548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4541..4563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4690..5145
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4777..4796)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4987..5008
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5171..5275
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5276..5344,5345..6136)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5494..5513)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6307..6895
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6796..6815
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7049..7066
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7183..7204
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7219..7224,7225..7242,7243..7249)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7257..7273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7262..7284
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7281..7297
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7281..7297
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7316..7336
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7318..7336
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7364..7590
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7591..7771
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7818..7943
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8436..8669
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8854..8898
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9073..9573
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9492..9510
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9585..9980
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    10040..10157
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
       61 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      121 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      181 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      241 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      301 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      361 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
      421 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
      481 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
      541 gggaggtcta tataagcaga gctctctggc taactgtcgg gatcaacaag tttgtacaaa
      601 aaagcaggca ccatgctgcc ctttctgctg gccacactgg gcaccacagc cctcaacaac
      661 agcaacccca aggactactg ctacagcgcc cgcatccgca gcactgtcct gcagggcctg
      721 ccctttgggg gcgtccccac cgtgctggct ctcgacttca tgtgcttcct tgcactgctg
      781 ttcttattct ctatcctccg gaaggtggcc tgggactatg ggcggctggc cttggtgaca
      841 gatgcagaca ggcttcggcg gcaggagagg gaccgagtgg aacaggaata tgtggcttca
      901 gctatgcacg gggacagcca tgaccggtat gagcgtctca cctctgtctc cagctccgtt
      961 gactttgacc aaagggacaa tggtttctgt tcctggctga cagccatctt caggataaag
     1021 gatgatgaga tccgggacaa atgtgggggc gatgccgtgc actacctgtc ctttcagcgg
     1081 cacatcatcg ggctgctggt ggttgtgggc gtcctctccg taggcatcgt gctgcctgtc
     1141 aacttctcag gggacctgct ggagaacaat gcctacagct ttgggagaac caccattgcc
     1201 aacttgaaat cagggaacaa cctgctatgg ctgcacacct ccttcgcctt cctgtatctg
     1261 ctgctcaccg tctacagcat gcgtagacac acctccaaga tgcgctacaa ggaggatgat
     1321 ctggtgaagc ggaccctctt catcaatgga atctccaaat atgcagagtc agaaaagatc
     1381 aagaagcatt ttgaggaagc ctaccccaac tgcacagttc tcgaagcccg cccgtgttac
     1441 aacgtggctc gcctaatgtt cctcgatgca gagaggaaga aggccgagcg gggaaagctg
     1501 tacttcacaa acctccagag caaggagaac gtgcctacca tgatcaaccc caagccctgt
     1561 ggccacctct gctgctgtgt ggtgcgaggc tgtgagcagg tggaggccat tgagtactac
     1621 acaaagctgg agcagaagct gaaggaagac tacaagcggg agaaggagaa ggtgaatgag
     1681 aagcctcttg gcatggcctt tgtcaccttc cacaatgaga ctatcaccgc catcatcctg
     1741 aaggacttca acgtgtgtaa atgccagggc tgcacctgcc gtggggagcc acgcccctca
     1801 tcctgcagcg agtccctgca catctccaac tggaccgtgt cctatgcccc tgaccctcag
     1861 aacatctact gggagcacct ctccatccga ggcttcatct ggtggctgcg ctgcctggtc
     1921 atcaatgtcg tcctcttcat cctcctcttc ttcctcacca ctccagccat catcatcacc
     1981 accatggaca agttcaacgt caccaagcct gtggagtacc tcaacaaccc catcatcacc
     2041 cagttcttcc ccaccctgct gctgtggtgc ttctcggccc tccttcccac catcgtctac
     2101 tactcagcct tctttgaagc ccactggaca cgctctgggg agaacaggac aaccatgcac
     2161 aagtgctaca ctttcctcat cttcatggtg ctgctcctac cctcgctggg actgagcagc
     2221 ctggacctct tcttccgctg gctctttgat aagaaattct tggctgaggc agctattcgg
     2281 tttgagtgtg tgttcctgcc cgacaacggc gccttcttcg tgaactacgt cattgcctca
     2341 gcctttatcg gcaacgccat ggacctgctg cgcatcccag gcctgctcat gtacatgatc
     2401 cggctctgcc tggcgcgctc ggccgccgag aggcgcaacg tgaagcggca tcaggcctac
     2461 gagttccagt ttggcgcagc ctacgcctgg atgatgtgcg tcttcacggt ggtcatgacc
     2521 tacagtatca cctgccccat catcgtgccc ttcgggctca tgtacatgct gctgaagcac
     2581 ctggtagaca ggtacaatct ctactacgcc tacctgccgg ccaagctgga caagaagatc
     2641 cactcggggg ctgtgaacca ggtggtggcc gcgcccatcc tctgcctctt ctggctgctc
     2701 ttcttttcca ccatgcgcac ggggttccta gctcccacgt ctatgttcac atttgtggtc
     2761 ctggtcatca ccatcgtcat ctgtctctgc cacgtctgct ttggacactt caaatacctc
     2821 agtgcccaca actacaagat tgagcacacg gagacagata ctgtggaccc cagaagcaat
     2881 ggacggcccc ccactgctgc tgctgtcccc aaatctgcga aatacatcgc tcaggtgctg
     2941 caggactcag aggtggacgg ggatggggat ggggctcctg ggagctcagg ggatgagccc
     3001 ccatcatcct catcccaaga tgaggagttg ctgatgccac ccgacgccct cacggacaca
     3061 gacttccagt cttgcgagga cagcctcata gagaatgaga ttcaccagac agctttcttg
     3121 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa
     3181 tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat tacaaaattt
     3241 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     3301 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     3361 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     3421 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     3481 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     3541 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     3601 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
     3661 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     3721 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     3781 tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca aggcagctgt
     3841 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     3901 aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg
     3961 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4021 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4081 cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc atcttattat
     4141 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     4201 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     4261 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     4321 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     4381 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     4441 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     4501 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     4561 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     4621 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     4681 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     4741 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     4801 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     4861 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     4921 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     4981 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5041 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5101 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     5161 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5221 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5281 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5341 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5401 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5461 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5521 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5581 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5641 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5701 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5761 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5821 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     5881 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     5941 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6001 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6061 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6121 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6181 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6241 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6301 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6361 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6421 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6481 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6541 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6601 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6661 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6721 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6781 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6841 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     6901 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     6961 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     7021 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     7081 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     7141 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     7201 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     7261 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     7321 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
     7381 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
     7441 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
     7501 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
     7561 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
     7621 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7681 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7741 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7801 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7861 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7921 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7981 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     8041 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     8101 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     8161 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8221 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8281 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     8341 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     8401 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     8461 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     8521 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     8581 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     8641 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     8701 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     8761 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     8821 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     8881 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     8941 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     9001 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     9061 ccgaggggac ccttgcgcct tttccaaggc agccctgggt ttgcgcaggg acgcggctgc
     9121 tctgggcgtg gttccgggaa acgcagcggc gccgaccctg ggtctcgcac attcttcacg
     9181 tccgttcgca gcgtcacccg gatcttcgcc gctacccttg tgggcccccc ggcgacgctt
     9241 cctgctccgc ccctaagtcg ggaaggttcc ttgcggttcg cggcgtgccg gacgtgacaa
     9301 acggaagccg cacgtctcac tagtaccctc gcagacggac agcgccaggg agcaatggca
     9361 gcgcgccgac cgcgatgggc tgtggccaat agcggctgct cagcagggcg cgccgagagc
     9421 agcggccggg aaggggcggt gcgggaggcg gggtgtgggg cggtagtgtg ggccctgttc
     9481 ctgcccgcgc ggtgttccgc attctgcaag cctccggagc gcacgtcggc agtcggctcc
     9541 ctcgttgacc gaatcaccga cctctctccc cagggggtac caccatggcc aagcctttgt
     9601 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     9661 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     9721 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     9781 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     9841 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     9901 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     9961 ctggttatgt gtgggagggc ctgcagctgc agtagtaaga attctagatc ttgagacaaa
    10021 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10081 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10141 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    10201 tcgagggggc ccggggtt
//
