LOCUS       sequence-319726-        9450 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses CNGA4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9450)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 9450)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_CNGA4_OE_1
FEATURES             Location/Qualifiers
     source          1..9450
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             14..194
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(195..421)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(449..469)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(449..467)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(488..504)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(488..504)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(501..523)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    512..528
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(536..542,543..560,561..566))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    581..602
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(719..736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(890..1478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(970..989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1649..2440,2441..2509))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2272..2291
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2510..2614)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2640..3095)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2777..2798)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2989..3008
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3222..3244
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3236..3253
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3237..3253
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3263..3282
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3263..3281
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      3302..3437
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3477..3598
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3488..3507)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3542..3561
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             3676..3909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    3981..4569
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4087..4098
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4496..4516
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4611..4652)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4654..4678
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6407..6431)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(6454..6657)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6483..6503)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6658..6961
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7078..7195
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7255..7650)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     promoter        complement(7662..8162)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7725..7743)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             complement(8337..8381)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8566..8799
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9292..9417
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
ORIGIN
        1 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
       61 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
      121 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
      181 aaccagagag acccgtttat gtatcgagct aggcacttaa atacaatatc tctgcaatgc
      241 ggcaattcag tggttcgtcc aatccatgtc agacccgtct gttgccttcc taataaggca
      301 cgatcgtacc accttacttc caccaatcgg catgcacggt gctttttctc tccttgtaag
      361 gcatgttgct aactcatcgt taccatgttg caagactaca agagtattgc ataagactac
      421 attaagcttg cagctccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt
      481 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
      541 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      601 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      661 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      721 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      781 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      841 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      901 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      961 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1021 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1081 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     1141 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     1201 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     1261 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     1321 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     1381 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     1441 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     1501 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     1561 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     1621 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     1681 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     1741 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     1801 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     1861 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     1921 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     1981 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     2041 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     2101 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     2161 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     2221 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     2281 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     2341 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     2401 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     2461 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     2521 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     2581 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta
     2641 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     2701 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     2761 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     2821 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     2881 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     2941 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     3001 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     3061 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa
     3121 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     3181 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     3241 aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtacgtccct
     3301 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg
     3361 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
     3421 ggagttaggg gcgggatggg cggagttagg ggcgggatag ctagagccag acatgataag
     3481 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     3541 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttcc
     3601 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     3661 aactactata cgtactgcta gagattttcc acactgacta aaagggtctg agggatctct
     3721 agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa ggcaagcttt
     3781 attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct cagatctggt
     3841 ctaaccagag agacccagta caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta
     3901 gcccttccag tccccccttt tcttttaaaa agtggctaag atctacagct gccttgtaag
     3961 tcattggtct taaagtcgac gcggggaggc ggcccaaagg gagatccgac tcgtctgagg
     4021 gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc
     4081 cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc
     4141 aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca cggaattgtc
     4201 agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc
     4261 cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat
     4321 gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac
     4381 aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat
     4441 gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag
     4501 cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag
     4561 aggttgattg tcgacttaac gcgccaccgg ttagcgctag ctcattacta cgtagaatcg
     4621 agaccgagga gagggttagg gataggctta ccaaccactt tgtacaagaa agttggcaac
     4681 tctggacctg ggggtccctc ctggctggcc ctgccctctt catctttgga agttccctcc
     4741 tcaggctcac cctcgtcatc agcctcagcc aggtcctcgg gcattggcca ctctcgagtc
     4801 tgccactcca gccgttcaat gcggtaagca atcttaagtg cgctggactc cagctcagcc
     4861 aggaggcgag caaacttggt ctgtagatca tccagctgct ggtctaggcc tcgtagccgg
     4921 gactctgtgg cctcctgcag ggcgatctca gctgcctcag cattcacgtc caacttgttc
     4981 attttcagca ggatctcacg tcctttctcc tccatgatgg tctgtgcttg tggatactcg
     5041 ctcagcacct cccgcaggtc ctccttgctc aggcagaata ggtctgaata acctaggctc
     5101 ttgatgttgg ctgtgcggcg gttcccagac atgttccctt tgatgttgat gatgctgatc
     5161 tccccaaagt agagccctgc accgagcaca gcatactgtg tgataccatc atctgccacc
     5221 acggccagtt gaccctctcg gatgatgtac atctcttggc caatgtctcc tttgcggcat
     5281 acatattcac ctggtgagta ggtctggggc tgcagcttca gcaccagctc ctccagcagg
     5341 ctggcctcac agttctgaaa gatctgcacc cggctcagag tggacaggtg cacagacaca
     5401 gccacttctg cccgcagccg ctcaggcaag tgctgtaaga tggctacctc gttggtcatc
     5461 ttcttgttga tctgcaggtg ctgataccag tcaataactc gccgctccag cttgcggttg
     5521 acgtgctgca gcttcatgta cttcttcacc agtgcatgat ctgggtagaa agccgcatct
     5581 gcagtgttca tgttgtagat gacagagctc atgctaccca tgatggtggc gaaacccatg
     5641 acggccagca ggaagtcgcc caccatgaag aggtactctt cttccctggc tggcggcggt
     5701 gtatcgccca ctgtagtcag tatcagcgtg gagaagtaaa agctatagag gtactggcgc
     5761 cgcaggcgct caaagccagg ctgcgcgggg tccgggtaca cccatgcgtc acgcccgaag
     5821 cccaggtacc gggatagggc aaagtatagg cagctgttcc aatggatgac gacaaaaatg
     5881 taaagcatca gcttggcaat gcgaaaggca tttgggtaag ctgtgcgggt ctctgtgcgg
     5941 tcgaaggcct cgaagaggcg gggcgcgcgg agaaagcggt tcagcctcag ggtgggtgtg
     6001 tgcgggccca gccgcacgta gaccacatct gtgggcatca gggaagccag gtccaagaag
     6061 aaactccagg tgcgaacgta gcgactcgag atcctaccct tgtccaccac caggatgccc
     6121 tgttccaaga atcctgtgtg gaagcgcacc accatgtcta gtaggtatag caggtcactc
     6181 gtgtagtcca gcaccaacca ggccaccaga taaccgtgct gcaagtcggg gaagcaggct
     6241 ctgcacacga ggatgatgag gttatacatg actgggaaga ccattgtgtt cagccaccag
     6301 tagtagtaat ccccagatgg gtccaggaca ggcagcaact tcctggcctt ggatggggct
     6361 gggggactgg actctgttgt cttcactttg gtgtcctggc tcatggtgcc aacttttttg
     6421 tacaaacttg ttgatcccga cagttagcca gagagctctg cttatataga cctcccaccg
     6481 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     6541 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     6601 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     6661 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     6721 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     6781 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     6841 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     6901 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     6961 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataacc
     7021 ccgggccccc tcgagccggc gccaaagtgg atctctgctg tccctgtaat aaacccgaaa
     7081 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     7141 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     7201 gatgaatact gccatttgtc tcaagatcta gaattcttac tactgcagct gcaggccctc
     7261 ccacacataa ccagagggca gcaattcacg aatcccaact gccgtcggct gtccatcact
     7321 gtccttcact atggctttga tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc
     7381 gcaggggctc aagatgcccc tgttctcatt tccgatcgcg acgatacaag tcaggttgcc
     7441 agctgccgca gcagcagcag tgcccagcac cacgagttct gcacaaggtc ccccagtaaa
     7501 atgatataca ttgacaccag tgaagatgcg gccgtcgcta gagagagctg cgctggcgac
     7561 gctgtagtct tcagagatgg ggatgctgtt gattgtagcc gttgctcttt caatgagggt
     7621 ggattcttct tgagacaaag gcttggccat ggtggtaccc cctggggaga gaggtcggtg
     7681 attcggtcaa cgagggagcc gactgccgac gtgcgctccg gaggcttgca gaatgcggaa
     7741 caccgcgcgg gcaggaacag ggcccacact accgccccac accccgcctc ccgcaccgcc
     7801 ccttcccggc cgctgctctc ggcgcgccct gctgagcagc cgctattggc cacagcccat
     7861 cgcggtcggc gcgctgccat tgctccctgg cgctgtccgt ctgcgagggt actagtgaga
     7921 cgtgcggctt ccgtttgtca cgtccggcac gccgcgaacc gcaaggaacc ttcccgactt
     7981 aggggcggag caggaagcgt cgccgggggg cccacaaggg tagcggcgaa gatccgggtg
     8041 acgctgcgaa cggacgtgaa gaatgtgcga gacccagggt cggcgccgct gcgtttcccg
     8101 gaaccacgcc cagagcagcc gcgtccctgc gcaaacccag ggctgccttg gaaaaggcgc
     8161 aagggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     8221 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     8281 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     8341 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     8401 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     8461 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     8521 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     8581 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     8641 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     8701 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     8761 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     8821 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     8881 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc tccaggtctg
     8941 aagatcagcg gccgcttgct gtgcggtggt cttacttttg ttttgctctt cctctatctt
     9001 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     9061 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     9121 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccaggattaa ctgcgaatcg
     9181 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     9241 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     9301 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     9361 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     9421 ctctggtttc cctttcgctt tcaagtccct
//
