LOCUS       sequence-319728-        8748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses LRRC55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8748)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_LRRC55_OE_1
FEATURES             Location/Qualifiers
     source          1..8748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        58..361
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        362..565
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     516..536
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    588..612
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1639..1663)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1665..1706
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    1748..2336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1801..1821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2219..2230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2408..2641
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2719..2840
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2756..2775)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2810..2829
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      2880..3015
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3035..3054)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3036..3054)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3064..3081)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3064..3080)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3073..3095)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3222..3677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3309..3328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3519..3540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3703..3807
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3808..3876,3877..4668)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4026..4045)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4839..5427
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5328..5347
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5581..5598
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5715..5736
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5751..5756,5757..5774,5775..5781)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5789..5805
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5794..5816
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5813..5829
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5813..5829
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5848..5868
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        5850..5868
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5896..6122
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6123..6303
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6350..6475
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6968..7201
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7386..7430
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7605..8105
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8024..8042
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8117..8512
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8572..8689
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt
       61 tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac
      121 gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg
      181 ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag
      241 tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat
      301 gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat
      361 ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt
      421 tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga
      481 ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg
      541 gtgggaggtc tatataagca gagctctctg gctaactgtc gggatcaaca agtttgtaca
      601 aaaaagttgg caccatgctc agaagcccca ccttcacaga cgctggcccc agatgtagct
      661 gccttcctgt gtcacagact ctcgattcca tggacacagt cctcatgggc tccctccagc
      721 actgctgttg cctgctgcct aagatgggtg acacttgggc ccagcttccc tggcccgggc
      781 caccccaccc agcaatgctg ctgatctccc tcctcttggc agccgggttg atgcactcgg
      841 atgccggcac cagctgcccc gtcctttgca catgccgtaa ccaggtggtg gattgtagca
      901 gccagcggct attctccgtg cccccagacc tgccaatgga cacccgaaac ctcagcctgg
      961 cccacaaccg catcacagca gtgccgcctg gctacctcac atgctacatg gagctccagg
     1021 tgctggattt gcacaacaac tccttaatgg agctgccccg gggcctcttc ctccatgcca
     1081 agcgcttggc acacttggac ctgagctaca acaatttcag ccatgtgcca gccgacatgt
     1141 tccaggaggc ccatgggcta gtccacatcg acctgagcca caacccctgg ctgcggaggg
     1201 tgcatcccca ggcctttcag ggcctcatgc agctccgaga cctggacctc agttatgggg
     1261 gcctggcctt cctcagcctg gaggctcttg agggcctacc ggggctggtg accctgcaga
     1321 tcggtggcaa tccctgggtg tgtggctgca ccatggaacc cctgctgaag tggctgcgaa
     1381 accggatcca gcgctgtaca gcagattctc agctggctga gtgccggggc cctcctgaag
     1441 tcgagggcgc cccgctcttc tcactcactg aggagagctt caaggcctgc cacctgaccc
     1501 tgaccctgga tgattaccta ttcattgcgt ttgtgggctt cgtggtctcc attgcttctg
     1561 tggccaccaa cttcctcctg ggcatcactg ccaactgctg ccaccgctgg agcaaggcca
     1621 gtgaagagga agagatcttg ccaactttct tgtacaaagt ggttggtaag cctatcccta
     1681 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
     1741 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     1801 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     1861 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     1921 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     1981 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     2041 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     2101 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     2161 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     2221 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     2281 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     2341 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     2401 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     2461 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     2521 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     2581 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     2641 agtacgtata gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     2701 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     2761 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     2821 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     2881 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     2941 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     3001 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     3061 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3121 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3181 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3241 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3301 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3361 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3421 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3481 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3541 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3601 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3661 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     3721 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3781 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3841 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3901 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3961 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4021 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4081 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4141 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4201 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4261 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4321 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4381 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4441 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     4501 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4561 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4621 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4681 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4741 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4801 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4861 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4921 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4981 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5041 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5101 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5161 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5221 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5281 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5341 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5401 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     5461 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     5521 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5581 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     5641 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     5701 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     5761 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     5821 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     5881 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     5941 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     6001 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     6061 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     6121 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6181 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6241 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6301 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     6361 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     6421 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     6481 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     6541 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     6601 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     6661 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     6721 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     6781 gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag
     6841 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     6901 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     6961 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     7021 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     7081 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     7141 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     7201 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     7261 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     7321 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     7381 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     7441 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     7501 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     7561 caccattatc gtttcagacc cacctcccaa ccccgagggg acccttgcgc cttttccaag
     7621 gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg aaacgcagcg
     7681 gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc cggatcttcg
     7741 ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt
     7801 ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc actagtaccc
     7861 tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca
     7921 atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg
     7981 cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc gcattctgca
     8041 agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc gacctctctc
     8101 cccagggggt accaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     8161 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     8221 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     8281 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     8341 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     8401 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     8461 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gcctgcagct
     8521 gcagtagtaa gaattctaga tcttgagaca aatggcagta ttcatccaca attttaaaag
     8581 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     8641 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     8701 cagggacagc agagatccac tttggcgccg gctcgagggg gcccgggg
//
