LOCUS       sequence-319730-       10143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpresses TMEM63C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10143)
  TITLE     These plasmids allow lentivirus-mediated overexpression of 
            understudied V5 tagged ion channels in mammalian cell lines.
REFERENCE   2  (bases 1 to 10143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IDG_TMEM63C_OE_1
FEATURES             Location/Qualifiers
     source          1..10143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..308
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     463..483
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    535..559
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2073..2084)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(2981..3005)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3007..3048
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    3090..3678
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3143..3163)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3561..3572)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3750..3983
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4061..4182
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4098..4117)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4152..4171
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4222..4357
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4377..4396)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4378..4396)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4406..4423)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4406..4422)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4415..4437)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4564..5019
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4651..4670)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4861..4882
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5045..5149
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5150..5218,5219..6010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5368..5387)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6181..6769
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6670..6689
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6923..6940
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7057..7078
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7093..7098,7099..7116,7117..7123)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7131..7147
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7136..7158
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7155..7171
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7155..7171
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7190..7210
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7192..7210
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7238..7464
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7465..7645
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7692..7817
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8310..8543
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8728..8772
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8947..9447
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9366..9384
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9459..9854
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9914..10031
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactgtcggg atcaacaagt
      541 ttgtacaaaa aagttggcac catgtctgcc tcaccagacg acctgagtac agggggaagg
      601 ttacagaaca tgacagtgga tgaatgcttc cagtctcgga acaccgtcct ccaggggcag
      661 ccctttgggg gtgtccccac cgtgctgtgc ctcaacatcg ccctgtgggt gctcgtcctt
      721 gtggtttact ccttcctccg gaaagctgcg tgggactatg ggcgcctggc tctgctgata
      781 cacaatgaca gcctgacctc gctgatctat ggggagcaga gcgagaagac atctccctcg
      841 gagacttcct tggagatgga acgcagagac aagggattct gttcctggtt cttcaacagc
      901 ataacaatga aggacgagga tctgattaac aagtgtgggg acgacgcgcg catctacatc
      961 gtgttccagt accacctcat catctttgtg ctcatcatct gtatcccctc cctgggcatc
     1021 attttgccca tcaactatac tggatctgtt ctggactgga gcagtcactt tgctcggacc
     1081 accattgtca atgtctccac agagagcaag ctcctgtggc tgcatagcct gctgtccttc
     1141 ttctacttca tcaccaactt catgttcatg gctcatcact gcctggggtt tgcgcccagg
     1201 aatagccaaa aggtcacaag gacactaatg atcacctatg tgcccaagga cattgaagac
     1261 ccagaactca tcattaagca ttttcacgag gcctatccag gcagtgtcgt gacaagagtc
     1321 cacttctgct acgacgtcag gaacctgatc gacttggacg atcagaggcg ccatgccatg
     1381 cggggccggc ttttctatac agccaaggcc aagaagactg ggaaggtgat gatcaggatc
     1441 cacccctgtg cccgcctgtg cttctgcaag tgctggacct gcttcaagga ggtggatgca
     1501 gagcagtatt acagcgagct agaggagcag ctaacggacg agttcaacgc cgagctcaac
     1561 cgcgtgccgc tcaagcggct ggacctgatc tttgtcacct tccaggactc caggatggcc
     1621 aagcgtgtcc gtaaggatta caagtatgtc cagtgtggtg tgcaacccca gcagtcctca
     1681 gtgaccacca tcgtcaaatc atattactgg agggtcacta tggccccaca ccccaaagac
     1741 attatttgga aacacctgtc tgtccgccgc ttcttttggt gggcccgctt tatcgcaatc
     1801 aacaccttcc tcttcttcct cttcttcttt ctcaccacgc ctgccatcat catgaacact
     1861 atcgacatgt acaacgtcac ccgccccatc gagaagctgc agaacccaat tgtgacccag
     1921 ttcttcccct ctgtgatgct ctggggcttc acagtgatac tgcctctgat tgtctacttc
     1981 tccgccttcc tcgaggccca ctggaccaga tcaagtcaga atctggtcat ggtgcacaag
     2041 tgctacatct ttctggtgtt catggtagtc attctgccct ctatgggact gaccagtttg
     2101 gatgtctttc tccgctggct ctttgacatc tactatctag agcaagcatc catcaggttc
     2161 cagtgtgtgt tcctgccaga caacggcgcc ttctttgtca actacgtgat cacggcagct
     2221 ttacttggca caggcatgga gctgctgcgt ctggggtcac tcttctgcta cagcacccgc
     2281 ctcttcttct ctagatcaga gccagagaga gtcaacatca gaaagaacca ggccatagac
     2341 ttccagtttg ggcgtgagta tgcgtggatg atgaacgtgt tcagcgtggt gatggcgtac
     2401 agcatcactt gccccatcat tgtgcctttt gggttgctct acctgtgcat gaagcacttg
     2461 acggatcgct ataacatgta ctactccttt gcacccacca aactgaacga gcagatccac
     2521 atggctgccg tctcccaggc catctttgcg ccactcttgg gtctgttctg gatgctgttc
     2581 ttctccatcc tgcggttggg ttctctccac gccatcacca tcttttccct gtccaccctc
     2641 ctcattgcca tggtgattgc ctttgttggc atttttctgg ggaagcttcg gatggttgcc
     2701 gactacgagc ccgaggagga ggagatccag acagtgtttg acatggagcc aagcagcacc
     2761 tcctccacgc ccacctccct cctgtatgtg gccaccgtgc tgcaagaacc ggagttgaat
     2821 ctgacccccg cctcctcccc agccaggcac acctatggca ccatgaacaa ccagccggaa
     2881 gagggagaag aagagagtgg tctgaggggc tttgcgaggg agctagactc ggcccagttc
     2941 caggaagggc tggaactgga gggccagaac cagtaccact tgccaacttt cttgtacaaa
     3001 gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct
     3061 agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggattacaaa atttgtgaaa
     3121 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     3181 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     3241 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     3301 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     3361 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     3421 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     3481 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     3541 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     3601 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     3661 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     3721 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     3781 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     3841 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3901 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3961 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     4021 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     4081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     4201 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4261 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     4321 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     4381 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     4441 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     4501 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     4561 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4621 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4681 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4741 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     4801 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4861 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     4921 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     4981 ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa
     5041 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5101 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5161 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     5221 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     5281 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     5341 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     5401 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     5461 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     5521 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     5581 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     5641 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     5701 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     5761 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     5821 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5881 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5941 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6001 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6061 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6121 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6181 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     6241 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     6301 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     6361 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     6421 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     6481 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     6541 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     6601 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     6661 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     6721 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     6781 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6841 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6901 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6961 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7021 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7081 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7141 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     7201 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     7261 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     7321 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     7381 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     7441 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     7501 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     7561 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     7621 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     7681 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     7741 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     7801 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     7861 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     7921 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     7981 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     8041 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     8101 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     8161 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     8221 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     8281 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     8341 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     8401 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     8461 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     8521 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     8581 actgctgtgt cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     8641 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     8701 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     8761 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     8821 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     8881 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     8941 ggacccttgc gccttttcca aggcagccct gggtttgcgc agggacgcgg ctgctctggg
     9001 cgtggttccg ggaaacgcag cggcgccgac cctgggtctc gcacattctt cacgtccgtt
     9061 cgcagcgtca cccggatctt cgccgctacc cttgtgggcc ccccggcgac gcttcctgct
     9121 ccgcccctaa gtcgggaagg ttccttgcgg ttcgcggcgt gccggacgtg acaaacggaa
     9181 gccgcacgtc tcactagtac cctcgcagac ggacagcgcc agggagcaat ggcagcgcgc
     9241 cgaccgcgat gggctgtggc caatagcggc tgctcagcag ggcgcgccga gagcagcggc
     9301 cgggaagggg cggtgcggga ggcggggtgt ggggcggtag tgtgggccct gttcctgccc
     9361 gcgcggtgtt ccgcattctg caagcctccg gagcgcacgt cggcagtcgg ctccctcgtt
     9421 gaccgaatca ccgacctctc tccccagggg gtaccaccat ggccaagcct ttgtctcaag
     9481 aagaatccac cctcattgaa agagcaacgg ctacaatcaa cagcatcccc atctctgaag
     9541 actacagcgt cgccagcgca gctctctcta gcgacggccg catcttcact ggtgtcaatg
     9601 tatatcattt tactggggga ccttgtgcag aactcgtggt gctgggcact gctgctgctg
     9661 cggcagctgg caacctgact tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga
     9721 gcccctgcgg acggtgccga caggtgcttc tcgatctgca tcctgggatc aaagccatag
     9781 tgaaggacag tgatggacag ccgacggcag ttgggattcg tgaattgctg ccctctggtt
     9841 atgtgtggga gggcctgcag ctgcagtagt aagaattcta gatcttgaga caaatggcag
     9901 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     9961 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10021 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggctcgagg
    10081 gggcccgggg ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10141 agt
//
