LOCUS       sequence-319739-       11782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and 
            mOrange.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11782)
  TITLE     Cohen Lab SLP001 plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)
FEATURES             Location/Qualifiers
     source          1..11782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1606..1723
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1798..2245
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             2262..3020
                     /codon_start=1
                     /product="achaerhodopsin-3 (Arch) derivative with improved 
                     voltage sensitivity (Hochbaum et al., 2014)"
                     /label=QuasAr2
                     /translation="MVSIALQAGYDLLGDGRPETLWLGIGTLLMLIGTFYFLVRGWGVT
                     DKDAREYYAVTILVSGIASAAYLSMFFGIGLTEVSVGGEMLDIYYARYAQWLFTTPLLL
                     LHLALLAKVDRVTIGTLVGVDALMIVTGLIGALSHTAIARYSWWLFSTICMIVVLYVLA
                     TSLRSAAKERGPEVASTFNTLTALVLVLWTAYPILWIIGTEGAGVVGLGIETLLFMVLD
                     VTAKVGFGFILLRSRAILGDTEAPEPSAGAD"
     CDS             3027..3734
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEYNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             3828..3884
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3885..4829
                     /codon_start=1
                     /product="blue light-activated optogenetic actuator derived
                     from Scherffelia dubia channelrhodopsin (Hochbaum et al., 
                     2014)"
                     /label=CheRiff
                     /translation="GGAPAPDAHSAPPGNDSAGGSEYHAPAGYQVNPPYHPVHGYEEQC
                     SSIYIYYGALWEQETARGFQWFAVFLSALFLAFYGWHAYKASVGWEEVYVCSVELIKVI
                     LEIYFEFTSPAMLFLYGGNITPWLRYAEWLLTCPVILIHLSNITGLSEAYNKRTMALLV
                     SDLGTICMGVTAALATGWVKWLFYCIGLVYGTQTFYNAGIIYVESYYIMPAGGCKKLVL
                     AMTAVYYSSWLMFPGLFIFGPEGMHTLSVAGSTIGHTIADLLSKNIWGLLGHFLRIKIH
                     EHIIMYGDIRRPVSSQFLGRKVDVLAFVTEEDKV"
     CDS             join(4899..4901,4902..5615)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(4899..4901,4902..5612)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(4941..4962)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5202..5221)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5549..5570
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5640..6228
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5693..5713)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6111..6122)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6231..6247)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6232..6248)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6753..6933
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6959..6976)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6965..7189
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7235..7663
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7322..7341)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7532..7553
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7672..7692)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7677..8006
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7857..7992
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7919..7938
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8054..8101
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8120..8494
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8624..8745
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8661..8680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8715..8734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8794..8810)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8794..8810)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8807..8829)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8818..8834
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8842..8848,8849..8866,8867..8872))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8887..8908
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9025..9042)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9196..9784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9276..9295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9955..10746,10747..10815))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10578..10597
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10816..10920)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10995..11014)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11186..11565
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11567..11765
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11720..11740
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     1561 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     1621 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     1681 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     1741 cagcagagat ccagtttggt taattaagtg tctagactgc agagggccct gcgtatgagt
     1801 gcaagtgggt tttaggacca ggatgaggcg gggtgggggt gcctacctga cgaccgaccc
     1861 cgacccactg gacaagcacc caacccccat tccccaaatt gcgcatcccc tatcagagag
     1921 ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg ccagcttcag caccgcggac
     1981 agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc ctcagcactg aaggcgcgct
     2041 gacgtcactc gccggtcccc cgcaaactcc ccttcccggc caccttggtc gcgtccgcgc
     2101 cgccgccggc ccagccggac cgcaccacgc gaggcgcgag ataggggggc acgggcgcga
     2161 ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag tctgcggtgg gcagcggagg
     2221 agtcgtgtcg tgcctgagag cgcagtcgag aaggatccac catggtaagt atcgctctgc
     2281 aggctggtta cgacctactg ggtgacggca gacctgaaac cctgtggctg ggcatcggca
     2341 ctctgctgat gctgattgga accttctact ttctggtccg cggatgggga gtcaccgata
     2401 aggatgcccg ggaatattac gctgtgacta tcctggtgtc cggaatcgca tccgccgcat
     2461 atctgtctat gttctttggt atcgggctta ctgaggtgtc cgtcgggggc gaaatgttgg
     2521 atatctatta tgccaggtac gcccagtggc tgtttaccac cccacttctg ctgctgcacc
     2581 tggcccttct cgctaaggtg gatcgggtga ccatcggcac cctggtgggt gtggacgccc
     2641 tgatgatcgt cactggcctc atcggagcct tgagccacac ggccatagcc agatacagtt
     2701 ggtggttgtt ctctacaatt tgcatgatag tggtgctcta tgttctggct acatccctgc
     2761 gatctgctgc aaaggagcgg ggccccgagg tggcatctac ctttaacacc ctgacagctc
     2821 tggtcttggt gctgtggacc gcttacccta tcctgtggat cataggcact gagggcgctg
     2881 gcgtggtggg cctgggcatc gaaactctgc tgtttatggt gttggacgtg actgccaagg
     2941 tcggctttgg ctttatcctg ttgagatccc gggctattct gggcgacacc gaggcaccag
     3001 aacccagtgc cggtgccgat acgttgatgg tgagcaaggg cgaggagtat aacatggcca
     3061 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
     3121 tcgagatcga gggcgagggc gagggccgcc cctacgaggg ctttcagacc gctaagctga
     3181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct catttcacct
     3241 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttc aagctgtcct
     3301 tccccgaggg cttcaagtgg gagcgcgtga tgaactacga ggacggcggc gtggtgaccg
     3361 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
     3421 ccaacttccc ctccgacggc cccgtgatgc agaagaagac catgggctgg gaggcctcct
     3481 ccgagcggat gtaccccgag gacggtgccc tgaagggcaa gatcaagatg aggctgaagc
     3541 tgaaggacgg cggccactac acctccgagg tcaagaccac ctacaaggcc aagaagcccg
     3601 tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcacctcc cacaacgagg
     3661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
     3721 acgagctgta caagagtaga atcacaagcg aaggcgagta catccccctg gatcaaatag
     3781 acataaatgt aggtggattt tgttatgaga atgaagtggg ctccggagcc acgaacttct
     3841 ctctgttaaa gcaagcagga gacgtggaag aaaaccccgg tcccggcgga gctcctgctc
     3901 cagacgctca cagcgcccca cctggaaacg attctgccgg aggcagtgag taccatgccc
     3961 cagctggata tcaagtgaat ccaccctacc accccgtgca tgggtatgag gaacagtgca
     4021 gctccatcta catctactat ggggccctgt gggagcagga aacagctagg ggcttccagt
     4081 ggtttgccgt gttcctgtct gccctgtttc tggctttcta cggctggcac gcctataagg
     4141 ccagcgtggg atgggaggaa gtgtacgtgt gctccgtgga gctgatcaaa gtgattctgg
     4201 agatctattt cgagttcacc agtcctgcta tgctgttcct gtacggaggg aacattaccc
     4261 catggctgag atatgccgaa tggctgctga catgtcccgt gatcctgatt catctgtcta
     4321 acatcaccgg cctgagtgag gcatacaata agcggacaat ggctctgctg gtgtccgacc
     4381 tgggaactat ttgcatggga gtgacagccg ctctggccac tgggtgggtg aagtggctgt
     4441 tttactgtat cggcctggtg tatggaaccc agacattcta caacgctgga atcatctacg
     4501 tggagtctta ctatatcatg cctgccggcg gctgtaagaa actggtgctg gccatgactg
     4561 ccgtgtacta ttctagttgg ctgatgtttc ccggcctgtt catctttggg cctgaaggca
     4621 tgcacaccct gagcgtggct gggtccacta ttggccatac catcgccgac ctgctgtcca
     4681 agaatatttg gggactgctg gggcacttcc tgcggatcaa aattcacgag catatcatta
     4741 tgtacggcga tatcaggaga ccagtgagct cccagtttct gggacgcaag gtggacgtgc
     4801 tggccttcgt gacagaggaa gataaagtgg cggccgccaa gagcaggatc accagcgagg
     4861 gcgagtacat ccccctggac cagatcgaca tcaacgtggt gagcaagggc gaggagctgt
     4921 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     4981 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcattt
     5041 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     5101 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     5161 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     5221 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     5281 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     5341 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     5401 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     5461 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     5521 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     5581 ggatcactct cggcatggac gagctgtaca agtaagaatt cgatatcaag cttatcgata
     5641 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5701 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     5761 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     5821 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     5881 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     5941 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6001 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     6061 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6121 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6181 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     6241 gacctcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
     6301 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     6361 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     6421 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     6481 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     6541 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     6601 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     6661 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     6721 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     6781 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     6841 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     6901 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     6961 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     7021 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     7081 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     7141 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     7201 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     7261 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     7321 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7381 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7441 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7501 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7561 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7621 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     7681 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7741 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     7801 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     7861 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     7921 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     7981 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     8041 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     8101 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     8161 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     8221 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     8281 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     8341 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     8401 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     8461 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     8521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     8581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     8641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8701 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     8761 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     8821 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     8881 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     8941 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     9001 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     9061 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     9121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     9181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     9241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     9301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     9361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     9421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     9481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     9541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     9601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     9661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     9721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     9781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     9841 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     9901 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     9961 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    10021 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    10081 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    10141 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    10201 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    10261 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    10321 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    10381 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    10441 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    10501 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    10561 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    10621 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    10681 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    10741 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    10801 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    10861 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
    10921 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
    10981 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
    11041 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
    11101 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
    11161 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
    11221 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
    11281 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
    11341 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
    11401 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
    11461 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
    11521 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
    11581 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
    11641 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
    11701 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
    11761 agcagcgcgt tttgcctgta ct
//
