LOCUS       sequence-319740-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-144~451 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir144~451-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    564..645
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(601..620)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(656..673)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gggatcctct agtcagctga cgcgtgctag
       61 cgcggccgcg ttctgccccc agcccctcac agtgcttttc aagccatgct tcctgtgccc
      121 ccagtggggc cctggctggg atatcatcat atactgtaag tttgcgatga gacactacag
      181 tatagatgat gtactagtcc gggcaccccc agctctggag cctgacaagg aggacaggag
      241 agatgctgca agcccaagaa gctctctgct cagcctgtca caacctactg actgccaggg
      301 cacttgggaa tggcaaggaa accgttacca ttactgagtt tagtaatggt aatggttctc
      361 ttgctatacc cagaaaacgt gccaggaaga gaactcagga ccctgaagca gactactgga
      421 agggagactc caaagcttgt cgacgatatc tccagaggat cataatcagc cataccacat
      481 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
      541 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
      601 gcaatagcat cacaaatttc acaaataaag catttttttc actgccccga gcttcctcgc
      661 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
      721 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgag
//
