LOCUS       sequence-319764-         559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-451 and miR-1-1~144 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir451-CMV2-mir1~144
FEATURES             Location/Qualifiers
     source          1..559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    389..470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(426..445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(481..498)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatataagca gagctggttt agtgaaccgt cagatccgct agggatcctc tagtcagctg
       61 acgcgtgcta gcgcggccgc aagaagctct ctgctcagcc tgtcacaacc tactgactgc
      121 cagggcactt gggaatggca aggaaaccgt taccattact gagtttagta atggtaatgg
      181 ttctcttgct atacccagaa aacgtgccag gaagagaact caggaccctg aagcagacta
      241 ctggaaggga gactccaaag cttgtcgacg atatctccag aggatcataa tcagccatac
      301 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      361 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc cccgagcttc
      481 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      541 aaaggcggta atacggtta
//
