LOCUS       sequence-319769-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-451~144 and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir451~144-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..57
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     8..28
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    611..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(648..667)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(703..720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctggt
       61 ttagtgaacc gtcagatccg ctagggatcc tctagtcagc tgacgcgtgc tagcgcggcc
      121 gcaagaagct ctctgctcag cctgtcacaa cctactgact gccagggcac ttgggaatgg
      181 caaggaaacc gttaccatta ctgagtttag taatggtaat ggttctcttg ctatacccag
      241 aaaacgtgcc aggaagagaa ctcaggaccc tgaagcagac tactggaagg gagactccag
      301 ttctgccccc agcccctcac agtgcttttc aagccatgct tcctgtgccc ccagtggggc
      361 cctggctggg atatcatcat atactgtaag tttgcgatga gacactacag tatagatgat
      421 gtactagtcc gggcaccccc agctctggag cctgacaagg aggacaggag agatgctgca
      481 agcttgtcga cgatatctcc agaggatcat aatcagccat accacatttg tagaggtttt
      541 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
      601 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
      661 aaatttcaca aataaagcat ttttttcact gccccgagct tcctcgctca ctgactcgct
      721 gcgctcggtc
//
