LOCUS       sequence-319772-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning 
            site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Rozen-Gagnon K, Yi S, Jacobson E, Novack S, Rice CM
  TITLE     A selectable, plasmid-based system to generate CRISPR/Cas9 gene 
            edited and knock-in mosquito cell lines.
  JOURNAL   Sci Rep. 2021 Jan 12;11(1):736. doi: 10.1038/s41598-020-80436-5.
  PUBMED    33436886
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2021-01-12"; volume: "11(1):736. doi"; pages: " 
            10.1038/s41598-020-80436-5"
COMMENT     Sequence Label: pKRG3-mU6-PUb-hSpCas9-pAc
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_RNA        194..269
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     regulatory      1840..1849
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1852..1872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1897..5997
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1897..5997
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5998..6045
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             6061..6114
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6139..6738
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVWPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6139..6158)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6635..6655
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6743..6767)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          6859..6924
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7054..7074
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7499..7633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7536..7555)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7590..7609
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(7730..7798,7799..8590)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7948..7967)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8761..9349
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9250..9269
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9503..9520
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gggttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca gcgaatgaaa tcgcccatcg agttgatacg tccatccatc
      121 gctagaaccg cgttcgctgt agaagactat ataagagcag aggcaagagt agtgaaatgg
      181 agacgacgtc tctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      241 aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt
      301 aaaataaggc tagtccgttt ttagcgcttg cgccaattct gcagacaaat ggctatcttt
      361 acatgtagct tgtgcattga atccaattat aatttgcctt ggcaccagct gagccagaca
      421 agaaagaaag cttcccagaa gtatatcgat ttagaagggt tgacgtcact tgctgactgc
      481 actaatacag caaatgatgc aattagaatg attcaagtga aattcccaaa ttactgattt
      541 ttctctggat ttggttatca gattacattc gaagctaaga ttagctaccg aaattgtcga
      601 tcaaatcagg aaatcctttc tctatcgaaa aaggcattcg cacatcttcc tctctatgcc
      661 atatacacga agggtaggta cattgacgtc tttgccagaa gttgaactgc atcgttcaag
      721 gtacagaatg aacgactaac agacacaagc acgttttgct gtccattcag acacagggat
      781 ggtacccata ttcgatcgat atagagccat ccaaccgaac agaggtatat gtatgaatgt
      841 attgctgaaa ttttctagaa gtacaaccac cactacgaca gtgtctataa aacgcccctg
      901 caaaggcgaa accagctcaa tcgaatacgt ttcctagtgg agtgaacatt acgcggccca
      961 agtaagcagt gccagtgcaa gtgaagtgaa gtctctagtg aaaaagagtg atccaattag
     1021 ccagaggaga aaatttcaga gtgaacaaag ctttattcaa aggacaatta ctattaaatt
     1081 ggtgaaagtg catttcggtg aagggaatct tctagtgaag gtaggtaaat taattgatga
     1141 aattatagct atgagcgaaa actagtttgg tgaatgattc ctttgtcttt gaatgagcaa
     1201 actattttcc aagatggcga ctattgagct ttgagtgatt agtgaaaatt tgcaacgcag
     1261 tttcatcatc attgataaaa cccaattgtg attcacagcg ataatcatat ttcgttgaat
     1321 catcgctact aattgaatta aatttctaga ataataagaa taacgtattt gctccgtcac
     1381 atatctaaaa taaatatttt gatggtaatt acccattaag gtaatattaa cacatatcga
     1441 gaaaaacctt gaggaaatcg tgaaaacttg aagatacgca atttcaaaac tacgtagttc
     1501 aaagtcgaaa acaagttaat ttttcactta aaagtagggc gttgttgtga cgtcatcacc
     1561 ttcaagtgta tatttttcac ttggcctgcg actgcaaacg cagacaaagc aaaacaagtt
     1621 taaaacctgt cgtgtcgtgc tcgaagccaa aggcaatgaa tcaatatcaa atgagagttt
     1681 gcatttcaca accaattact caagcgtttc ctcgtttctt tttctgctca acagagattt
     1741 caacaccaag ctcgataagc ttgttcgaat ctcgagtgcg cgcttccgga ggtatacacc
     1801 taggcggtac cactgcagtg aattcggagc tctaccggtg ccaccatggc cccaaagaag
     1861 aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag catcggcctg
     1921 gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     1981 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     2041 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     2101 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     2161 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     2221 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     2281 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     2341 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     2401 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     2461 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     2521 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     2581 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     2641 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     2701 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     2761 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     2821 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     2881 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     2941 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     3001 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     3061 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     3121 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     3181 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     3241 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     3301 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     3361 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     3421 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     3481 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     3541 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     3601 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     3661 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     3721 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     3781 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     3841 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     3901 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     3961 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     4021 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     4081 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     4141 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     4201 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     4261 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     4321 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     4381 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     4441 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     4501 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     4561 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     4621 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     4681 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     4741 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     4801 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     4861 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     4921 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     4981 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     5041 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     5101 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     5161 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     5221 atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     5281 aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     5341 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     5401 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     5461 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     5521 cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc
     5581 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     5641 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     5701 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     5761 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     5821 atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta ctttgacacc
     5881 accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac cctgatccac
     5941 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggcgacaaa
     6001 aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagggatc aggcaagctt
     6061 gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctgctagc
     6121 ggtagcggca gcggtagcat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     6181 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6241 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6301 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6361 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6421 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6481 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctg gcccgaccac
     6541 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6601 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6661 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6721 cgcaagcccg gtgcctaata ggacccagct ttcttgtaca aagtggtgac gtaagctagc
     6781 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     6841 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     6901 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     6961 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     7021 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagttgac
     7081 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     7141 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     7201 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     7261 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     7321 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     7381 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7441 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     7501 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7561 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7621 tcatgtctgg atcccgttta aactacgcgt aattcaaaca gggttctggc gtcgttctcg
     7681 tactgttttc cccaggccag tgctttagcg ttattgaaaa aggaagagta tgagtattca
     7741 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7801 cccagaaacg ctggtgaaag taaaggatgc tgaagatcag ttgggtgcac gagtgggtta
     7861 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     7921 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     7981 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8041 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8101 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8161 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8221 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8281 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8341 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8401 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8461 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8521 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8581 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8641 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8701 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8761 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8821 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     8881 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     8941 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9001 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9061 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9121 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9181 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9241 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9301 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9361 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9421 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9481 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gagaaga
//
