LOCUS       sequence-319780-        8587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LuxCDABE operon (R6K ori) under T7, in loxP and lox5171 sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8587)
  AUTHORS   Wang G, Zhao Z, Ke J, Engel Y, Shi YM, Robinson D, Bingol K, Zhang 
            Z, Bowen B, Louie K, Wang B, Evans R, Miyamoto Y, Cheng K, Kosina S,
            De Raad M, Silva L, Luhrs A, Lubbe A, Hoyt DW, Francavilla C, Otani 
            H, Deutsch S, Washton NM, Rubin EM, Mouncey NJ, Visel A, Northen T, 
            Cheng JF, Bode HB, Yoshikuni Y
  TITLE     CRAGE enables rapid activation of biosynthetic gene clusters in 
            undomesticated bacteria.
  JOURNAL   Nat Microbiol. 2019 Dec;4(12):2498-2510. doi: 
            10.1038/s41564-019-0573-8. Epub 2019 Oct 14.
  PUBMED    31611640
REFERENCE   2  (bases 1 to 8587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41564-019-0573-8"; journalName: "Nat Microbiol"; date: 
            "2019-12"; volume: "4"; issue: "12"; pages: "2498-2510"
COMMENT     Sequence Label: pW34
FEATURES             Location/Qualifiers
     source          1..8587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    20..44
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             59..81
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             89..1531
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1544..2467
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PNNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2516..3598
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3613..4596
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             4775..5887
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     primer_bind     5991..6011
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(6235..6253)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6249..6296
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(6385..6401)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6386..6402)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(7266..7368)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    7366..7399
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     7657..7679
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7671..7688
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7672..7688
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7713..7731)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     oriT            7776..7884
                     /note="incP origin of transfer"
     rep_origin      8040..8396
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     protein_bind    complement(8439..8472)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     8522..8538
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8522..8538
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
       61 tgtttaactt taagaaggag atatacatat gactaaaaaa atttcattca ttattaacgg
      121 ccaggttgaa atctttcccg aaggtgatga tttagtgcaa tccattaatt ttggtgataa
      181 tagtgtttac ctgccaatat tgaatgactc tcatgtaaaa aacattattg attgtaatgg
      241 aaataacgaa ttacggttgc ataacattgt caattttctc tatacggtag ggcaaagatg
      301 gaaaaatgaa gaatactcaa gacgcaggac atacattcgt gacttaaaaa aatatatggg
      361 atattcagaa gaaatggcta agctagaggc caattggata tctatgattt tatgttctaa
      421 aggcggcctt tatgatgttg tagaaaatga acttggttct cgccatatca tggatgaatg
      481 gctacctcag gatgaaagtt atgttcgggc ttttccgaaa ggtaaatctg tacatctgtt
      541 ggcaggtaat gttccattat ctgggatcat gtctatatta cgcgcaattt taactaagaa
      601 tcagtgtatt ataaaaacat cgtcaaccga tccttttacc gctaatgcat tagcgttaag
      661 ttttattgat gtagacccta atcatccgat aacgcgctct ttatctgtta tatattggcc
      721 ccaccaaggt gatacatcac tcgcaaaaga aattatgcaa catgcggatg ttattgtcgc
      781 ttggggaggg ccagatgcga ttaattgggc ggtagagcat gcgccatctt atgctgatgt
      841 gattaaattt ggttctaaaa agagtctttg cattatcgat aatcctgttg atttgacgtc
      901 cgcagcgaca ggtgcggctc atgatgtttg tttttacgat cagcgagctt gtttttctgc
      961 ccaaaacata tattacatgg gaaatcatta tgaggaattt aagttagcgt tgatagaaaa
     1021 acttaatcta tatgcgcata tattaccgaa tgccaaaaaa gattttgatg aaaaggcggc
     1081 ctattcttta gttcaaaaag aaagcttgtt tgctggatta aaagtagagg tggatattca
     1141 tcaacgttgg atgattattg agtcaaatgc aggtgtggaa tttaatcaac cacttggcag
     1201 atgtgtgtac cttcatcacg tcgataatat tgagcaaata ttgccttatg ttcaaaaaaa
     1261 taagacgcaa accatatcta tttttccttg ggagtcatca tttaaatatc gagatgcgtt
     1321 agcattaaaa ggtgcggaaa ggattgtaga agcaggaatg aataacatat ttcgagttgg
     1381 tggatctcat gacggaatga gaccgttgca acgattagtg acatatattt ctcatgaaag
     1441 gccatctaac tatacggcta aggatgttgc ggttgaaata gaacagactc gattcctgga
     1501 agaagataag ttccttgtat ttgtcccata ataggtaaaa agtatggaaa atgaatcaaa
     1561 atataaaacc atcgaccacg ttatttgtgt tgaaggaaat aaaaaaattc atgtttggga
     1621 aacgctgcca gaagaaaaca gcccaaagag aaagaatgcc attattattg cgtctggttt
     1681 tgcccgcagg atggatcatt ttgctggtct ggcggaatat ttatcgcgga atggatttca
     1741 tgtgatccgc tatgattcgc ttcaccacgt tggattgagt tcagggacaa ttgatgaatt
     1801 tacaatgtct ataggaaagc agagcttgtt agcagtggtt gattggttaa ctacacgaaa
     1861 aataaataac ttcggtatgt tggcttcaag cttatctgcg cggatagctt atgcaagcct
     1921 atctgaaatc aatgcttcgt ttttaatcac cgcagtcggt gttgttaact taagatattc
     1981 tcttgaaaga gctttagggt ttgattatct cagtctaccc attaatgaat tgccgaataa
     2041 tctagatttt gaaggccata aattgggtgc tgaagtcttt gcgagagatt gtcttgattt
     2101 tggttgggaa gatttagctt ctacaattaa taacatgatg tatcttgata taccgtttat
     2161 tgcttttact gcaaataacg ataattgggt caagcaagat gaagttatca cattgttatc
     2221 aaatattcgt agtaatcgat gcaagatata ttctttgtta ggaagttcgc atgacttgag
     2281 tgaaaattta gtggtcctgc gcaattttta tcaatcggtt acgaaagccg ctatcgcgat
     2341 ggataatgat catctggata ttgatgttga tattactgaa ccgtcatttg aacatttaac
     2401 tattgcgaca gtcaatgaac gccgaatgag aattgagatt gaaaatcaag caatttctct
     2461 gtcttaaaat ctattgagat attctatcac tcaaatagca atataaggac tctctatgaa
     2521 atttggaaac tttttgctta cataccaacc tccccaattt tctcaaacag aggtaatgaa
     2581 acgtttggtt aaattaggtc gcatctctga ggagtgtggt tttgataccg tatggttact
     2641 ggagcatcat ttcacggagt ttggtttgct tggtaaccct tatgtcgctg ctgcatattt
     2701 acttggcgcg actaaaaaat tgaatgtagg aactgccgct attgttcttc ccacagccca
     2761 tccagtacgc caacttgaag atgtgaattt attggatcaa atgtcaaaag gacgatttcg
     2821 gtttggtatt tgccgagggc tttacaacaa ggactttcgc gtattcggca cagatatgaa
     2881 taacagtcgc gccttagcgg aatgctggta cgggctgata aagaatggca tgacagaggg
     2941 atatatggaa gctgataatg aacatatcaa gttccataag gtaaaagtaa accccgcggc
     3001 gtatagcaga ggtggcgcac cggtttatgt ggtggctgaa tcagcttcga cgactgagtg
     3061 ggctgctcaa tttggcctac cgatgatatt aagttggatt ataaatacta acgaaaagaa
     3121 agcacaactt gagctttata atgaagtggc tcaagaatat gggcacgata ttcataatat
     3181 cgaccattgc ttatcatata taacatctgt agatcatgac tcaattaaag cgaaagagat
     3241 ttgccggaaa tttctggggc attggtatga ttcttatgtg aatgctacga ctatttttga
     3301 tgattcagac caaacaagag gttatgattt caataaaggg cagtggcgtg actttgtatt
     3361 aaaaggacat aaagatacta atcgccgtat tgattacagt tacgaaatca atcccgtggg
     3421 aacgccgcag gaatgtattg acataattca aaaagacatt gatgctacag gaatatcaaa
     3481 tatttgttgt ggatttgaag ctaatggaac agtagacgaa attattgctt ccatgaagct
     3541 cttccagtct gatgtcatgc catttcttaa agaaaaacaa cgttcgctat tatattagct
     3601 aaggagaaag aaatgaaatt tggattgttc ttccttaact tcatcaattc aacaactgtt
     3661 caagaacaaa gtatagttcg catgcaggaa ataacggagt atgttgataa gttgaatttt
     3721 gaacagattt tagtgtatga aaatcatttt tcagataatg gtgttgtcgg cgctcctctg
     3781 actgtttctg gttttctgct cggtttaaca gagaaaatta aaattggttc attaaatcac
     3841 atcattacaa ctcatcatcc tgtccgcata gcggaggaag cttgcttatt ggatcagtta
     3901 agtgaaggga gatttatttt agggtttagt gattgcgaaa aaaaagatga aatgcatttt
     3961 tttaatcgcc cggttgaata tcaacagcaa ctatttgaag agtgttatga aatcattaac
     4021 gatgctttaa caacaggcta ttgtaatcca gataacgatt tttatagctt ccctaaaata
     4081 tctgtaaatc cccatgctta tacgccaggc ggacctcgga aatatgtaac agcaaccagt
     4141 catcatattg ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat
     4201 tctaatgatg ttagatatga atatgctgaa agatataaag ccgttgcgga taaatatgac
     4261 gttgacctat cagagataga ccatcagtta atgatattag ttaactataa cgaagatagt
     4321 aataaagcta aacaagagac gcgtgcattt attagtgatt atgttcttga aatgcaccct
     4381 aatgaaaatt tcgaaaataa acttgaagaa ataattgcag aaaacgctgt cggaaattat
     4441 acggagtgta taactgcggc taagttggca attgaaaagt gtggtgcgaa aagtgtattg
     4501 ctgtcctttg aaccaatgaa tgatttgatg agccaaaaaa atgtaatcaa tattgttgat
     4561 gataatatta agaagtacca catggaatat acctaataga tttcgagttg cagcgaggcg
     4621 gcaagtgaac gaatccccag gagcatagat aactatgtga ctggggtgag tgaaagcagc
     4681 caacaaagca gcagcttgaa agatgaaggg tataaaagag tatgacagca gtgctgccat
     4741 actttctaat attatcttga ggagtaaaac aggtatgact tcatatgttg ataaacaaga
     4801 aattacagca agctcagaaa ttgatgattt gattttttcg agcgatccat tagtgtggtc
     4861 ttacgacgag caggaaaaaa tcagaaagaa acttgtgctt gatgcatttc gtaatcatta
     4921 taaacattgt cgagaatatc gtcactactg tcaggcacac aaagtagatg acaatattac
     4981 ggaaattgat gacatacctg tattcccaac atcggttttt aagtttactc gcttattaac
     5041 ttctcaggaa aacgagattg aaagttggtt taccagtagc ggcacgaatg gtttaaaaag
     5101 tcaggtggcg cgtgacagat taagtattga gagactctta ggctctgtga gttatggcat
     5161 gaaatatgtt ggtagttggt ttgatcatca aatagaatta gtcaatttgg gaccagatag
     5221 atttaatgct cataatattt ggtttaaata tgttatgagt ttggtggaat tgttatatcc
     5281 tacgacattt accgtaacag aagaacgaat agattttgtt aaaacattga atagtcttga
     5341 acgaataaaa aatcaaggga aagatctttg tcttattggt tcgccatact ttatttattt
     5401 actctgccat tatatgaaag ataaaaaaat ctcattttct ggagataaaa gcctttatat
     5461 cataaccgga ggcggctgga aaagttacga aaaagaatct ctgaaacgtg atgatttcaa
     5521 tcatctttta tttgatactt tcaatctcag tgatattagt cagatccgag atatatttaa
     5581 tcaagttgaa ctcaacactt gtttctttga ggatgaaatg cagcgtaaac atgttccgcc
     5641 gtgggtatat gcgcgagcgc ttgatcctga aacgttgaaa cctgtacctg atggaacgcc
     5701 ggggttgatg agttatatgg atgcgtcagc aaccagttat ccagcattta ttgttaccga
     5761 tgatgtcggg ataattagca gagaatatgg taagtatccc ggcgtgctcg ttgaaatttt
     5821 acgtcgcgtc aatacgagga cgcagaaagg gtgtgcttta agcttaaccg aagcgtttga
     5881 tagttgatga cctgcaggca tgcaagcttg cggccgcggg ccccatggat cgatagctgg
     5941 tcgacacaat ctgccctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt
     6001 gagtttgtaa ctgctgctgg gattacacat ggcatggatg agctctacaa ataatgagct
     6061 agcccgccta atgagcgggc ttttttttct cggcctagga gatacttaac agggaagtga
     6121 gagggccgcg gcaaagccgt ttttccatag gctccgcccc cctgacaagc atcacgaaat
     6181 ctgacgctca aatcagtggc aaagcccgaa aggaagctga gttggctgct gccaccgctg
     6241 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6301 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaaggct
     6361 gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccct
     6421 ccacgtgttg ccccagcaat cagcgcgacc ttgcccctcc aacgtcatct cgttctccgc
     6481 tcatgagctc agccaatcga ctggcgagcg gcatcgcatt cttcgcatcc cgcctctggc
     6541 ggatgcagga agatcaacgg atctcggccc agttgaccca gggctgtcgc cacaatgtcg
     6601 cgggagcgga tcaaccgagc aaaggcatga ccgactggac cttccttctg aaggctcttc
     6661 tccttgagcc acctgtccgc caaggcaaag cgctcacagc agtggtcatt ctcgagataa
     6721 tcgacgcgta ccaacttgcc atcctgaaga atggtgcagt gtctcggcac cccataggga
     6781 acctttgcca tcaactcggc aagatgcagc gtcgtgttgg catcgtgtcc cacgccgagg
     6841 agaagtacct gcccatcgag ttcatggaca cgggcgaccg ggcttgcagg cgagtgaggt
     6901 ggcaggggca atggatcaga gatgatctgc tctgcctgtg gccccgctgc cgcaaaggca
     6961 aatggatggg cgctgcgctt tacatttggc aggcgccaga atgtgtcaga gacaactcca
     7021 aggtccggtg taacgggcga cgtggcagga tcgaacggct cgtcgtccag acctgaccac
     7081 gagggcatga cgagcgtccc tcccggaccc agcgcagcac gcagggcctc gatcagtcca
     7141 agtggcccat cttcgagggg ccggacgcta cggaaggagc tgtggaccag cagcacaccg
     7201 ccgggggtaa ccccaaggtt gagaagctga ccgatgagct cggcttttcg ccattcgtat
     7261 tgcattttag cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt
     7321 gatcttattt cattatggtg aaagttggaa cctcttacgt gccgaataac ttcgtataat
     7381 gtgtactata cgaagttatt caacgtctca ttttcgccaa aagttggccc agggcttccc
     7441 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
     7501 ttattcgcga taagctcatg gagcggcgta accgtcgcac aggaaggaca gagaaagcgc
     7561 ggatctggga agtgacggac agaacggtca ggacctggag cggccgcttt tttccaattt
     7621 ttgcggccgc tatgtgttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7681 cggccagtga attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt
     7741 taatgtcatg ataataatgg tttcttagag cttatcggcc agcctcgcag agcaggattc
     7801 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
     7861 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
     7921 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
     7981 gctataatga ccccgaagca gggttatgca gcgaagatcc gcagttcaac ctgttgatag
     8041 tacgtactaa gctctcatgt ttcacgtact aagctctcat gtttaacgta ctaagctctc
     8101 atgtttaacg aactaaaccc tcatggctaa cgtactaagc tctcatggct aacgtactaa
     8161 gctctcatgt ttcacgtact aagctctcat gtttgaacaa taaaattaat ataaatcagc
     8221 aacttaaata gcctctaagg ttttaagttt tataagaaaa aaaagaatat ataaggcttt
     8281 taaagctttt aaggtttaac ggttgtggac aacaagccag ggatgtaacg cactgagaag
     8341 cccttagagc ctctcaaagc aattttgagt gacacaggaa cacttaacgg ctgacatggg
     8401 aattagccat ggcaaagcct cgcaatccag tgcaaaggat aacttcgtat agcatacatt
     8461 atacgaagtt atgataaccg tattaccgcc tttgagtgag ctccaccgcg gtggcggccg
     8521 ctctagaact agtggatccc catgcgtccg gcgtagagga tcgagatcga tctcgatccc
     8581 gcgaaat
//
