LOCUS       sequence-319783-        8854 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8854)
  AUTHORS   Hakre S, Tussie-Luna MI, Ashworth T, Novina CD, Settleman J, Sharp 
            PA, Roy AL
  TITLE     Opposing functions of TFII-I spliced isoforms in growth 
            factor-induced gene expression.
  JOURNAL   Mol Cell. 2006 Oct 20. 24(2):301-8.
  PUBMED    17052463
REFERENCE   2  (bases 1 to 8854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Oct 20. 24(2):301-8."
COMMENT     Sequence Label: pSFG-GFP
FEATURES             Location/Qualifiers
     source          1..8854
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             514..983
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1048..1405
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     1816..1838
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1854..1870
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     2208..2224
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    join(2283..2301,2302..2324,2325..2343,2344..2366,2367..2385,
                     2386..2408,2409..2427,2428..2450,2451..2469,2470..2492,
                     2493..2511,2512..2534,2535..2553)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2586..2624
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2621..2645
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(2711..2713,2714..2716,2717..3430)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2711..2713,2714..2716,2717..3427)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2756..2777)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3017..3036)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3364..3385
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(3445..4019,4020..4022,4023..4031)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3612..3629)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3839..3858
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4032..4652,4653..5039)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(4105..4124)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(6226..6243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6226..6242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6235..6257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6451..6470)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6570..6592
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6630..6648)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6716..6820
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6821..6889,6890..7681)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7039..7058)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7852..8440
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8341..8360
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8594..8611
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8728..8749
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8764..8769,8770..8787,8788..8794)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8802..8818
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8807..8829
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8826..8842
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8826..8842
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagctttgct cttaggagtt tcctaataca tcccaaactc aaatatataa agcatttgac
       61 ttgttctatg ccctaggggg cggggggaag ctaagccagc tttttttaac atttaaaatg
      121 ttaattccat tttaaatgca cagatgtttt tatttcataa gggtttcaat gtgcatgaat
      181 gctgcaatat tcctgttacc aaagctagta taaataaaaa tagataaacg tggaaattac
      241 ttagagtttc tgtcattaac gtttccttcc tcagttgaca acataaatgc gctgctgaga
      301 agccagtttg catctgtcag gatcaatttc ccattatgcc agtcatatta attactagtc
      361 aattagttga tttttatttt tgacatatac atgtgaaaga ccccacctgt aggtttggca
      421 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa
      481 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
      541 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
      601 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
      661 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
      721 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
      781 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggc
      841 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
      901 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
      961 tacccgtcag cgggggtctt tcatttgggg gctcgtccgg gatcgggaga cccctgccca
     1021 gggaccaccg acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga
     1081 ttgtctagtg tctatgactg attttatgcg cctgcgtcgg tactagttag ctaactagct
     1141 ctgtatctgg cggacccgtg gtggaactga cgagttcgga acacccggcc gcaaccctgg
     1201 gagacgtccc agggacttcg ggggccgttt ttgtggcccg acctgagtcc taaaatcccg
     1261 atcgtttagg actctttggt gcacccccct tagaggaggg atatgtggtt ctggtaggag
     1321 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa
     1381 gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1441 ttctgtattt gtctgaaaat atgggcccgg gctagcctgt taccactccc ttaagtttga
     1501 ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga
     1561 agagacgttg ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc
     1621 gagacggcac ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg
     1681 gcccgcatgg acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg
     1741 acccccctcc ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat
     1801 ccgccccgtc tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc
     1861 cagccctcac tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac
     1921 ccccgcccct tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc
     1981 tccaagctca cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg
     2041 cagcctacca agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca
     2101 cagtgtgggt ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca
     2161 cagtcctgct gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg
     2221 ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc ctctagccct cgagtttacc
     2281 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     2341 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2401 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccag tccctatcag
     2461 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2521 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct cggtacccgg
     2581 gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta gtgaaccgtc
     2641 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     2701 ccgacctgcc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     2761 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     2821 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     2881 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     2941 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3001 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3061 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3121 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3181 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3241 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     3301 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     3361 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     3421 gtacaagtaa agcggccgcg gatccccctc tccctccccc ccccctaacg ttactggccg
     3481 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc
     3541 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag
     3601 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt
     3661 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa
     3721 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc
     3781 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg
     3841 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat
     3901 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa
     3961 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataagc
     4021 ttgccacaac catgtctaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc
     4081 ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag
     4141 agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca
     4201 ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa agctggcaag
     4261 attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag
     4321 caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat
     4381 tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg
     4441 tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag
     4501 aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct atcgaattat
     4561 ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat
     4621 tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag ccgcgcgcgt acgaaaaaca
     4681 attacgggtc taccatcgag ggcctgctcg atctcccgga cgacgacgcc cccgaagagg
     4741 cggggctggc ggctccgcgc ctgtcctttc tccccgcggg acacacgcgc agactgtcga
     4801 cggccccccc gaccgatgtc agcctggggg acgagctcca cttagacggc gaggacgtgg
     4861 cgatggcgca tgccgacgcg ctagacgatt tcgatctgga catgttgggg gacggggatt
     4921 ccccgggtcc gggatttacc ccccacgact ccgcccccta cggcgctctg gatatggccg
     4981 acttcgagtt tgagcagatg tttaccgatg cccttggaat tgacgagtac ggtgggtagg
     5041 gggcgcgaag atccggatta gtccaatttg ttaaagacag gatatcagtg gtccaggctc
     5101 tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca tagataaaat
     5161 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     5221 gcaagctagc tcgaccctag agaaccatca gatgtttcca gggtgcccca aggacctgaa
     5281 atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc
     5341 ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc
     5401 gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga
     5461 cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg
     5521 gggtctttca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca
     5581 ttaaatggcc atagtactta aagttacatt ggcttccttg aaataaacat ggagtattca
     5641 gaatgtgtca taaatatttc taattttaag atagtatctc cattggcttt ctactttttc
     5701 ttttattttt ttttgtcctc tgtcttccat ttgttgttgt tgttgtttgt ttgtttgttt
     5761 gttggttggt tggttaattt ttttttaaag atcctacact atagttcaag ctagactatt
     5821 agctactctg taacccaggg tgaccttgaa gtcatgggta gcctgctgtt ttagccttcc
     5881 cacatctaag attacaggta tgagctatca tttttggtat attgattgat tgattgattg
     5941 atgtgtgtgt gtgtgattgt gtttgtgtgt gtgattgtgt atatgtgtgt atggttgtgt
     6001 gtgattgtgt gtatgtatgt ttgtgtgtga ttgtgtgtgt gtgattgtgc atgtgtgtgt
     6061 gtgtgattgt gtttatgtgt atgattgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     6121 gtgtgtgtgt gttgtgtata tatatttatg gtagtgagag gcaacgctcc ggctcaggtg
     6181 tcaggttggt ttttgagaca gagtctttca cttagcttgg aattcactgg ccgtcgtttt
     6241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6361 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     6421 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     6481 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     6541 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     6601 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6661 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6721 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6781 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6841 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6901 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6961 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7021 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     7081 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     7141 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     7201 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     7261 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     7321 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     7381 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     7441 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     7501 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     7561 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     7621 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7681 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7741 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7801 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7861 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7921 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7981 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     8041 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     8101 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     8161 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     8221 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     8281 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     8341 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     8401 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     8461 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     8521 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     8581 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     8641 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8701 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8761 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8821 tcacacagga aacagctatg accatgatta cgcc
//
