LOCUS       sequence-319789-        9063 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transgene expression driven by ALPHA MHC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Gulick J, Subramaniam A, Neumann J, Robbins J
  TITLE     Isolation and characterization of the mouse cardiac myosin heavy 
            chain genes.
  JOURNAL   J Biol Chem. 1991 May 15;266(14):9180-5.
  PUBMED    2026617
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1991-05-15"; volume: "266(14)"; pages: "9180-5"
COMMENT     Sequence Label: pJG/ALPHA MHC
FEATURES             Location/Qualifiers
     source          1..9063
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     77..93
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    5551..6173
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5750..5769)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(6188..6204)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6276..6294)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6315..6331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6315..6331)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6328..6350)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6339..6355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6363..6369,6370..6387,6388..6393))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6408..6429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6546..6563)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6717..7305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6797..6816)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7476..8267,8268..8336))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8099..8118
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8337..8441)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8467..8922)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8604..8625)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8816..8835
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(9049..9063,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9063,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtgaa ttgtaatacg actcactata gggcgaattg ggtacctcca
       61 ccgcggtggc ggccgctcta gaactagtgg atcctgcaag gtcacacaag ggtctccacc
      121 caccaggtgc cctagtctca atttcagttt ccatgccttg ttctcacaat gctggcctcc
      181 ccagagctaa tttggacttt gtttttattt caaaagggcc tgaatgagga gtagatcttg
      241 tgctacccag ctctaagggt gcccgtgaag ccctcagacc tggagccttt gcaacagccc
      301 tttaggtgga agcagaataa agcaattttc cttaaagcca aaatcctgcc tctagactct
      361 tcttctctga cctcggtccc tgggctctag ggtggggagg tggggcttgg aagaagaagg
      421 tggggaagtg gcaaaagccg atccctaggg ccctgtgaag ttcggagcct tccctgtaca
      481 gcactggctc atagatcctc ctccagccaa acatagcaag aagtgatacc tcctttgtga
      541 cttccccagg cccagtacct gtcaggttga aacaggattt agagaagcct ctgaactcac
      601 ctgaactctg aagctcatcc accaagcaag cacctaggtg ccactgctag ttagtatcct
      661 acgctgataa tatgcagagc tgggccacag aagtcctggg gtgtaggaac tgaccagtga
      721 cttttcagtc ggcaaaggta tgaccccctc agcagatgta gtaatgtccc cttagatccc
      781 atcccaggca ggtctctaag aggacatggg atgagagatg tagtcatgtg gcattccaaa
      841 cacagctatc cacagtgtcc cttgcccctt ccacttagcc aggaggacag taaccttagc
      901 ctatctttct tcctccccat cctcccagga cacaccccct ggtctgcagt attcatttct
      961 tccttcacgt cccctctgtg acttccattt gcaaggcttt tgacctctgc agctgctgga
     1021 agatagagtt tggccctagg tgtggcaagc catctcaaga gaaagcagac aacaggggga
     1081 ccagattttg gaaggatcag gaactaaatc actggcgggc ctgggggtag aaaaaagagt
     1141 gagtgagtcc gctccagcta agccaagcta gtccccgaga tactctgcca cagctgggct
     1201 gctcggggta gctttaggaa tgtgggtctg aaagacaatg ggattggaag acatctcttt
     1261 gagtctcccc tcaaccccac ctacagacac actcgtgtgt ggccagactc ctgttcaaca
     1321 gccctctgtg ttctgaccac tgagctaggc aaccagagca tgggccctgt gctgaggatg
     1381 aagagttggt taccaatagc aaaaacagca ggggagggag aacagagaac gaaataagga
     1441 aggaagaagg aaaggccagt caatcagatg cagtcagaag agatgggaag ccaacacaca
     1501 gcttgagcag aggaaacaga aaagggagag attctgggca taaggaggcc acagaaagaa
     1561 gagcccaggc cccccaagtc tcctctttat accctcatcc cgtctcccaa ttaagcccac
     1621 tcttcttcct agatcagacc tgagctgcag cgaagagacc cgtagggagg atcacactgg
     1681 atgaaggaga tgtgtggaga agtccaggga acctaagagc cagagcctaa aagagcaaga
     1741 gataaaggtg cttcaaaggt ggccaggctg tgcacacaga gggtcgagga ctggtggtag
     1801 agcctcaaga taaggatgat gctcagaatg ggcggggggg gggattctgg gggggggaga
     1861 gagaaggtga gaaggagcct ggaacagaga atctggaagc gctggaaacg ataccataaa
     1921 gggaagaacc caggctacct ttagatgtaa atcatgaaag acagggagaa gggaagctgg
     1981 agagagtaga aggaccccgg ggcaagacat ggaagcaagg acaagccagg ttgagcgctc
     2041 cgtgaaatca gcctgctgaa ggcagagccc tggtatgagc accagaacag cagaggctag
     2101 ggttaatgtc gagacaggga acagaaggta gacacaggaa cagacagaga cgggggagcc
     2161 aggtaacaaa ggaatggtcc ttctcacctg tggccagagc gtccatctgt gtccacatac
     2221 tctagaatgt tcatcagact gcagggctgg cttgggaggc agctggaaag agtatgtgag
     2281 agccagggga gacaaggggg cctaggaaag gaagaagagg gcaaaccagg ccacacaaga
     2341 gggcagagcc cagaactgag ttaactcctt ccttgttgca tcttccatag gaggcagtgg
     2401 gaactctgtg accaccatcc cccatgagcc cccactaccc ataccaagtt tggcctgagt
     2461 ggcattctag gttccctgag gacagagcct ggcctttgtc tcttggacct gacccaagct
     2521 gacccaatgt tctcagtacc ttatcatgcc ctcaagagct tgagaaccag gcagtgacat
     2581 attaggccat gggctaaccc tggagcttgc acacaggagc ctcaagtgac ctccagggac
     2641 acagctgcag acaggtggcc tttatcccca aagagcaacc atttggcata ggtggctgca
     2701 aatgggaatg caaggttgaa tcaggtccct tcaagaatac tgcatgcaag acctaagacc
     2761 cctggagaga ggggtatgct cctgccccca cccaccataa ggggagtgaa ctatcctagg
     2821 gggctggcga ccttggggag acaccacatt actgagagtg ctgagcccag aaaaactgac
     2881 cgccctgtgt cctgcccacc tccacactct agagctatat tgagaggtga cagtagatag
     2941 ggtgggagct ggtagcaggg agagtgttcc tgggtgtgag ggtgtagggg aaagccagag
     3001 caggggagtc tggctttgtc tcctgaacac aatgtctact tagttataac aggcatgacc
     3061 tgctaaagac ccaacatcta cgacctctga aaagacagca gccctggagg acaggggttg
     3121 tctctgagcc ttgggtgctt gatggtgcca caaaggaggg catgagtgtg agtataaggc
     3181 cccaggagcg ttagagaagg gcacttggga aggggtcagt ctgcagagcc cctatccatg
     3241 gaatctggag cctggggcca actggtgtaa atctctgggc ctgccaggca ttcaaagcag
     3301 cacctgcatc ctctggcagc ctggggaggc ggaagggagc aaccccccac ttataccctt
     3361 tctccctcag ccccaggatt aacacctctg gccttccccc ttcccacctc ccatcaggag
     3421 tggagggttg cagagggagg gtaaaaacct acatgtccaa acatcatggt gcacgatata
     3481 tggatcagta tgtgtagagg caagaaagga aatctgcagg cttaactggg ttaatgtgta
     3541 aagtctgtgt gcatgtgtgt gtgtctgact gaaaacgggc atggctgtgc agctgttcag
     3601 ttctgtgcgt gaggttacca gactgcaggt ttgtgtgtaa attgcccaag gcaaagtggg
     3661 tgaatccctt ccatggttta aagagattgg atgatggcct gcatctcaag gaccatggaa
     3721 aatagaatgg acactctata tgtgtctcta agctaaggta gcaaggtctt tggaggacac
     3781 ctgtctagag atgtgggcaa cagagactac agacagtatc tgtacagagt aaggagagag
     3841 aggagggggt gtagaattct cttactatca aagggaaact gagtcgtgca cctgcaaagt
     3901 ggatgctctc cctagacatc atgactttgt ctctggggag ccagcactgt ggaacttcag
     3961 gtctgagaga gtaggaggct cccctcagcc tgaagctatg cagatagcca gggttgaaag
     4021 ggggaaggga gagcctggga tgggagcttg tgtgttggag gcaggggaca gatattaagc
     4081 ctggaagaga aggtgaccct tacccagttg ttcaactcac ccttcagatt aaaaataact
     4141 gaggtaaggg cctgggtagg ggaggtggtg tgagacgctc ctgtctctcc tctatctgcc
     4201 catcggccct ttggggagga ggaatgtgcc caaggactaa aaaaaggcca tggagccaga
     4261 ggggcgaggg caacagacct ttcatgggca aaccttgggg ccctgctgtc ctcctgtcac
     4321 ctccagagcc aagggatcaa aggaggagga gccaggacag gagggaagtg ggagggaggg
     4381 tcccagcaga ggactccaaa tttaggcagc aggcatatgg gatgggatat aaaggggctg
     4441 gagcactgag agctgtcaga gatttctcca acccaggtaa gagggagttt cgggtggggg
     4501 ctcttcaccc acaccagacc tctccccacc tagaaggaaa ctgcctttcc tggaagtggg
     4561 gttcaggccg gtcagagatc tgacagggtg gccttccacc agcctgggaa gttctcagtg
     4621 gcaggaggtt tccacaagaa acactggatg ccccttccct tacgctgtct tctccatctt
     4681 cctcctgggg atgctcctcc ccgtcttggt ttatcttggc tcttcgtctt cagcaagatt
     4741 tgccctgtgc tgtccactcc atctttctct actgtctccg tgccttgcct tgccttcttg
     4801 cgtgtccttc ctttccaccc atttctcact tcaccttttc tccccttctc atttgtattc
     4861 atccttcctt ccttccttcc ttccttcctt ccttccttcc ttccttcctt tctcccttcc
     4921 ttccttcctt ccttccttcc ttccttcctt ccttcctgtg tcagagtgct gagaatcaca
     4981 cctggggttc ccacccttat gtaaacaatc ttccagtgag ccacagcttc agtgctgctg
     5041 ggtgctctct taccttcctc accccctggc ttgtcctgtt ccatcctggt caggatctct
     5101 agattggtct cccagcctct gctactcctc ttcctgcctg ttcctctctc tgtccagctg
     5161 cgccactgtg gtgcctcgtt ccagctgtgg tccacattct tcaggattct ctgaaaagtt
     5221 aaccaggtga gaatgtttcc cctgtagaca gcagatcacg attctcccgg aagtcaggct
     5281 tccagccctc tctttctctg cccagctgcc cggcactctt agcaaacctc aggcaccctt
     5341 accccacata gacctctgac agagaagcag gcactttaca tggagtcctg gtgggagagc
     5401 cataggctac ggtgtaaaag aggcagggaa gtggtggtgt aggaaagtca ggacttcaca
     5461 tagaagccta gcccacacca gaaatgacag acagatccct cctatctccc ccataagagt
     5521 ttgagtcgac tgactaacta gaagcttgat gggtggcatc cctgtgaccc ctccccagtg
     5581 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     5641 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     5701 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     5761 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     5821 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     5881 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     5941 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     6001 tgaaccactg ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac
     6061 gtccagacac agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg
     6121 cttggcactg tcctctcatg cgttgggtcc actcagtaga tgcctgttga attcctgcag
     6181 cccgggggat ccactagttc tagagcggcc gtttaaacta gtatacacgt ggcgcccggg
     6241 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aatttcgagc
     6301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     6721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     6781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     6841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     6901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     7741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     7801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     7861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     7921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8461 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     8521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     8581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     8641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     8701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     8761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     8821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     8881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     8941 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     9001 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     9061 gtt
//
