LOCUS       sequence-319807-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express miR-125a and miR-1-1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Fang W, Bartel DP
  TITLE     MicroRNA Clustering Assists Processing of Suboptimal MicroRNA 
            Hairpins through the Action of the ERH Protein.
  JOURNAL   Mol Cell. 2020 Apr 16;78(2):289-302.e6. doi: 
            10.1016/j.molcel.2020.01.026.
  PUBMED    32302541
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01"; journalName: "Mol Cell"; date: 
            "2020-04-16- 16"; volume: "78"; issue: "2"; pages: "289-302.e6"
COMMENT     Sequence Label: pBI-CMV1-mir125-CMV2-mir1
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        12..63
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     14..34
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    544..625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(581..600)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(636..653)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggncta tataagcaga
       61 gctggtttag tgaaccgtca gatccgctag ggatcctcta gtcagctgac gcgtgctagc
      121 gcggccgccg tttttggtct ttctgtctct ggctctcaga atgtctctgt gcctatctcc
      181 atctctgacc cccaccccag ggtctaccgg gccaccgcac accatgttgc cagtctctag
      241 gtccctgaga ccctttaacc tgtgaggaca tccagggtca caggtgaggt tcttgggagc
      301 ctggcgtctg gcccaaccac acacctgggg aattgctggc ctgacttctg acccctgact
      361 cctcataccc ttcctccaga gcatgacatt tgaccaccaa ctgaaacctg acaagcttgt
      421 cgacgatatc tccagaggat cataatcagc cataccacat ttgtagaggt tttacttgct
      481 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
      541 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
      601 acaaataaag catttttttc actgccccga gcttcctcgc tcactgactc gctgcgctcg
      661 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
      721 gaatcagggg ataacgcagg aaagaacatg tgagcaaa
//
